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Open Access 01-12-2023 | Hematology | Review

CRISPR screening in hematology research: from bulk to single-cell level

Authors: Sarah Meyers, Sofie Demeyer, Jan Cools

Published in: Journal of Hematology & Oncology | Issue 1/2023

Abstract

The CRISPR genome editing technology has revolutionized the way gene function is studied. Genome editing can be achieved in single genes or for thousands of genes simultaneously in sensitive genetic screens. While conventional genetic screens are limited to bulk measurements of cell behavior, recent developments in single-cell technologies make it possible to combine CRISPR screening with single-cell profiling. In this way, cell behavior and gene expression can be monitored simultaneously, with the additional possibility of including data on chromatin accessibility and protein levels. Moreover, the availability of various Cas proteins leading to inactivation, activation, or other effects on gene function further broadens the scope of such screens. The integration of single-cell multi-omics approaches with CRISPR screening open the path to high-content information on the impact of genetic perturbations at single-cell resolution. Current limitations in cell throughput and data density need to be taken into consideration, but new technologies are rapidly evolving and are likely to easily overcome these limitations. In this review, we discuss the use of bulk CRISPR screening in hematology research, as well as the emergence of single-cell CRISPR screening and its added value to the field.

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Title: CRISPR screening in hematology research: from bulk to single-cell level
Authors: Sarah Meyers
Sofie Demeyer
Jan Cools
Publication date: 01-12-2023
Publisher: BioMed Central
Keyword: Hematology
Published in: Journal of Hematology & Oncology / Issue 1/2023
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/s13045-023-01495-5

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