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01-11-2018 | Original Article

Hemato-biochemcial characteristics of lambs on dietary feed additives (exogenous fibrolytic enzymes, Artemisia absinthium Linn.) supplementation

Authors: Yasir Afzal Beigh, Abdul Majeed Ganai, Masood Saleem Mir, Ishfaq Ahmad, Umar Amin, Farukh Mehraj

Published in: Comparative Clinical Pathology | Issue 6/2018

Abstract

Dietary composition affects blood metabolic profile of farm animals. Hemato-biochemical indices serve as clinical indicators for the impact of dietary manipulations on physiological health status. To augment sheep productivity in temperate regions by improving animal health conditions, the present study was undertaken to assess the effect of supplementation of exogenous fibrolytic enzymes (EFE) cocktail and Artemisia absinthium L. (commonly known as wormwood) herb alone and in combination as feed additives on blood metabolic profile in lambs. Crossbred lambs (n = 20) divided into four equal groups of five in each and fed for 90 days followed by 6 days of metabolism trial on oat straw-based complete feeds supplemented with either EFE cocktail (T₁) @ 6 g/kg DM or wormwood herb (T₂) @ 4.5% of DM or a combination of the two (T₃) and complete feed without any supplementation served as control (T₀). Hemato-biochemical parameters of all the animals were studied at 0-, 30-, 60-, and 90-day intervals. The feed additives supplementation improved the nutritive value of diets; however, the significant (P < 0.01) effect for percent digestible crude protein and digestible energy were with T₃ where combination of enzymes and herb was applied, whereas higher (P < 0.05) percent total digestible nutrients were recorded in groups supplemented with wormwood herb (T₂ and T₃) compared to control. Feed additive supplementations did not change significantly the mean observations of red blood cell (except hematocrit) and platelet indices, total blood cell counts, energy metabolic profile, serum total cholesterol and total triglycerides, serum total proteins, serum albumin, aspartate aminotransferase, serum creatinine, and uric acid levels; however, feed additive supplementation have a significant positive effect on differential WBC counts, high-density lipoproteins (P < 0.05), low-density lipoproteins, atherogenic index, serum globulin, and albumin: globulin ratio and serum urea nitrogen (P < 0.01) levels among the treatment groups. The hemato-biochemical metabolic profile of feed additive-supplemented lambs revealed better physiological health status compared to un-supplemented animals. Supplementations of EFE cocktail, wormwood herb, or their combination improved the nutritive value of diets without any adverse effects on hematological and blood biochemical parameter suggesting supplementation of the feed additives for successfully raising lambs intensively.

Adamu S, Thomas A, Iseh NM, Fatihumi MY, Esieno KAN (2006) Normal values of haematology of Nigeria adopted albino rats (Rattus norvegicus) in Zaria. In: Proc of 31st Annual Conf of Nig Soc for Anim prod (NSAP)

Addass PA, David DI, Edward A, Zira KE, Midak A (2012) Effect of age, sex and management system on some haematological parameters of intensively and semi-intensively kept chicken in Mubi, Adamawa State, Nigeria. Iran J Appl Anim Sci 2(3):277–282

Ajao BH, Ola SI, Adameji OV, Kolawole RF (2013) The relationship of ambient temperature and relative humidity of thermo respiratory function of greater grass cutter. In: Proc of the 18th Annual Conf of Anim Sci Assoc of Nig: 92

Amarenco P, Labreuche J, Touboul PJ (2008) High-density lipoprotein-cholesterol and risk of stroke and carotid atherosclerosis—a systematic review. Atheroscle 196:489–496CrossRef

Antunovic Z, Speranda M, Mioc B, Novoselec J, Speranda T (2009) Zum Ernahrungszustand von Ziegen unter organischen Produktionsbedingungen. Tierarztliche Umschau 64:18–23

AOAC (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, D.C.

Aro SO, Ogunwale FF, Falade OA (2013) Blood viscosity of finisher cockerel fed dietary inclusions of fermented cassava tuber wastes. In: Proc. of the 18th Annual Conf. of Anim Sci Assoc of Nig: 74–77

Aya VE, Ayanwale BA, Ijaiya AT, Aremu A (2013) Haematological and serum biochemistry indices of broiler chickens fed rumen filtrate fermented palm kernel meal based diet. In: Proc. of the 18^th annual Conf. of Anim Sci Assoc of Nig: 329

Baytop T (1984) Therapy with medicinal plants in Turkey. Istanbul University Press, Istanbul, Turkey, pp 166–167

Cronje PB, Pambu-Gollah R (1996) The use of blood profiles as animal response indicators. Bullet Grassland Soc South Africa 7 (Supplement 1): 28–35

Dean DB, Staples CR, Littell RC, Kim S, Adesogan AT (2013) Effect of method of adding a fibrolytic enzyme to dairy cow diets on feed intake digestibility, milk production, ruminal fermentation, and blood metabolites. Anim Nutr Feed Technol 13:337–353

El-Kady RI, Awadalla IM, Mohamed MI, Fadel M, El-Rahman HHA (2006) Effect of exogenous enzymes on the growth performance and digestibility of growing buffalo calves. Intern J Agric Biol 8(3):354–359

Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol without the use of the preparative ultracentrifuge. Clin Chem 18:499PubMed

Ghafari H, Khadem AA, Rezaeian M, Afzalzadeh A, Sharif D, Norouzian MA (2015) Effect of sesame oil feeding on performance, plasma lipids and ruminal fermentation of growing lambs. Iran J Vet Med 9(3):155–161

Gilani AH, Janbaz KH (1995) Preventive and curative effects of Artemisia absinthium on acetaminophen and CCl₄-induced hepatotoxicity. Gen Pharmacol 26:309–315CrossRef

Githiori JB, Hogland J, Waller PJ, Baker RL (2004) Evaluation of anthelmintic properties of some plants used as livestock dewormers against Haemonchus contortus infection in sheep. Parasit 129:245–253CrossRef

Gupta N, Kumar A, Tiwari DP (2006) Effect of herbs as feed additive on haemato-biochemical constituents in growing crossbred heifers fed paddy straw based ration. Indian J Anim Sci 76(7):528–531

Hong JC, Steiner T, Aufy A, Lien TF (2012) Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livest Sci 144:253–262CrossRef

Hosoda K, Kurumoto K, Eruden B, Nishida T, Shioya S (2006) The effect of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration and ruminal fermentation in Holstein steers. Asian Australas J Anim Sci 19(1):35–41CrossRef

Hussain A, Nisa M, Sarwar M, Harif M, Javaid A (2008) Effect of exogenous fibrolytic enzymes on ruminant performance. Pak J Agric Sci 45(2):297–306

ICAR (2013) Nutrient requirements of sheep, goat and rabbit. Indian Council of Agricultural Research. New Delhi, India

Jain NC (1986) Schalm’s veterinary haematology. 4th edition, Lea and Febiger, Washington square, Philadelphia, USA, pp. 208–239

Kaneko JJ, Harvey JW, Bruss ML (1997) Clinical biochemistry of domestic animals, 5th edn. Academic Press, San Diego

Khaki Z, Khazraiinia P, Chegini S, Nia SK (2012) Comparative study of serum lipid profile in chicken, ostrich, cattle, and sheep. Comp Clin Pathol 21:259–263CrossRef

Kim JH, Kim CH, Ko YD (2002) Influence of dietary addition of dried wormwood (Artemisia sp.) on the performance and carcass characteristics of Hanwoo steers and the nutrient digestibility of sheep. Asian Australas J Anim Sci 15(3):390–395CrossRef

Krause DO, Denman ST, Mackie RI, Morrison M, Rae AL, Attwood GT, McSweeney CS (2003) Opportunities to improve fibre degradation in the rumen: microbiology, ecology and genomics. FEMS Microbiol Rev 27:663–693CrossRef

Kris-Etherton PM, Yu S (1997) Individual fatty acid effects on plasma lipids and lipoproteins: human studies. Am J Clin Nutr 65:1628S–1644SCrossRef

Kurtoglu F, Kurtoglu V, Celik I, Kececi I, Nizamlioglu M (2005) Effect of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocytes, splenic plasma cells and bone characteristics of broiler chicks given diets with adequate or inadequate cholecalferol (vitamin D) content. Br Poult Sci 46:87–96CrossRef

Mahgoub O, Kadim IT, Tageldin MH, Al-Marzooqi WS, Khalaf SQ, Ambu-Ali A (2008) Clinical profile of sheep fed non-conventional feeds containing phenols and condensed tannins. Small Rumin Res 78:115–122CrossRef

Mandal GP, Roy A, Patra AK (2014) Effect of feeding plant additives rich in saponins and essential oils on the performance, carcass traits and conjugated linoleic acid concentration in muscle and adipose tissues of black Bengal goats. Anim Feed Sci Technol 197:76–84CrossRef

Morsy TA, Kholif AE, Kholif SM, Kholif AM, Sun X, Salem AZM (2016) Effects of two enzyme feed additives on digestion and milk production in lactating Egyptian buffaloes. Ann Anim Sci 16(1):209–222CrossRef

Nanda S, Tiwari SP, Rathore R (2013) Effect of polyherbal formulations on blood haematological constituents and immunity in non-descript goats. Iran J Appl Anim Sci 3(2):327–331

Omidi A, Rahdari S, Fard MH (2014) A preliminary study on antioxidant activities of saffron petal extracts in lambs. Vet Sci Dev 4:51–61CrossRef

Osawa T (1999) Protective role of dietary polyphenols in oxidative stress. Mech Ageing Dev 111:133–139CrossRef

Pambu-Gollah R, Cronje PB, Casey NH (2000) An evaluation of the use of blood metabolite concentrations as indicators of nutritional status in free-ranging indigenous goats. South African J Anim Sci 30(2):115–120CrossRef

Peters A, Meyer U, Danicke S (2015) Effect of exogenous fibrolytic enzymes on performance and blood profile in early and mid-lactation Holstein cows. Anim Nut 1:229–238CrossRef

Radostits OM, Gays CC, Blood DC (1994) Veterinary medicine: a textbook of disease of cattle, sheep, goats, pigs and horses. W. B. Saunders (eds.), pp. 304

Rafiu TA, Aderinola OA, Akinwumi AO, Alabi TA, Shittu MD (2013) Performance and blood chemistry of broiler chickens fed Moringa oleifera leaf meal. In: Proc. of the 18th Annual Conf of Anim Sci Assoc of Nig: 294

Rivero N, Salem AZM (2015) Biochemical parameters in sheep fed diet in presence of mixed Salix babylonica extract and exogenous enzyme as feed additives. Indian J Anim Sci 85(2):189–194

Rivero N, Salem AZM, Ayala M, Elghandour MMY, Kholif AE, Barbabosa A, Amacho LM, Rojas S, Jolivares CM (2016) Influence of Salix babylonica extract, exogenous enzyme of xylanase and their combination on blood haematological and biochemical profile in sheep and goats. Indian J Anim Sci 86(10):1140–1144

Rivero N, Salem AZM, Gado HM, Gonzalez-Ronquillo M, Pliego AB, Peñuelas CG, Odongo NE (2012) Effect of exogenous enzymes and Salix babylonica extract or their combination on haematological parameters in growing lambs. J Anim Feed Sci 21:577–586CrossRef

SPSS, version 20.0. Statistical Packages for Social Sciences, Software products, Marketing Department, SPSS Inc. Chicago, IL 60606-6307, USA.

Schiemann R, Nehring K, Hoffman L, Jentsch W, Chuddy A (1971) Energetische futter bewertung and Energinormen. VEM Dentsch Lant Wirte Chaftsverlag, Berlin

Vahora SG, Pande MB (2006) Effect of enzyme supplementation on feed utilization, blood constituents and reproduction in dairy cows. Indian J Anim Sci 76(6):471–475

Van Houtert MFJ, Sykes AR (1996) Implications of nutrition for the ability of ruminants to withstand gastrointestinal nematode infections. Intern J Parasit 26(11):1151–1167CrossRef

Title: Hemato-biochemcial characteristics of lambs on dietary feed additives (exogenous fibrolytic enzymes, Artemisia absinthium Linn.) supplementation
Authors: Yasir Afzal Beigh
Abdul Majeed Ganai
Masood Saleem Mir
Ishfaq Ahmad
Umar Amin
Farukh Mehraj
Publication date: 01-11-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 6/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2759-5

At a glance: The STEP trials

Springer Medicine

Hemato-biochemcial characteristics of lambs on dietary feed additives (exogenous fibrolytic enzymes, Artemisia absinthium Linn.) supplementation

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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