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01-01-2015

H₂O₂ Induces Myocardial Hypertrophy in H9c2 Cells: A Potential Role of Ube3a

Authors: Rui Song, Jie Zhang, Lijuan Zhang, Guanghua Wang, Da Wo, Jian Feng, Xucheng Li, Jue Li

Published in: Cardiovascular Toxicology | Issue 1/2015

Abstract

Myocardial hypertrophy that often leads to eventual heart failure is a leading cause of mortality worldwide. While both apoptosis and cell proliferation have been reported to play an important part in heart failure, its exact triggering mechanism is still unclear. Reports have shown that low concentrations of H₂O₂ (10–30 µM) can induce myocardial hypertrophy without affecting survival. The ubiquitin ligase Ube3a has been reported to have a close affiliation with Angelman syndrome; but many ubiquitin ligases have been reported in a variety of cardiovascular conditions including myocardial hypertrophy. However, the relationship between Ube3a and myocardial hypertrophy has never been reported in literature. The rat cardiac myoblast cell line H9c2 and primary neonatal cardiomyocytes showed similar hypertrophic responses in vitro. In this report, we utilized H₂O₂ treatment on H9c2 cells to induce myocardial hypertrophy and determined the relationship between Ube3a and myocardial hypertrophy. Our results showed that 10–20 μM H₂O₂ can induce myocardial hypertrophy without affecting cell viability and inducing cell apoptosis, while the corresponding transcription and translation levels of Ube3a are significantly increased during the process. Therefore, these findings underline that Ube3a may play an important role in myocardial hypertrophy.

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Title: H2O2 Induces Myocardial Hypertrophy in H9c2 Cells: A Potential Role of Ube3a
Authors: Rui Song
Jie Zhang
Lijuan Zhang
Guanghua Wang
Da Wo
Jian Feng
Xucheng Li
Jue Li
Publication date: 01-01-2015
Publisher: Springer US
Published in: Cardiovascular Toxicology / Issue 1/2015
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI: https://doi.org/10.1007/s12012-014-9264-0

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H₂O₂ Induces Myocardial Hypertrophy in H9c2 Cells: A Potential Role of Ube3a

Abstract

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Abstract

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