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Journal of Cardiovascular Translational Research

Published in:

01-04-2015

Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Authors: Neeta Adhikari, Kadambari Chandra Shekar, Rodney Staggs, Zaw Win, Kerianne Steucke, Yi-Wei Lin, Li-Na Wei, Patrick Alford, Jennifer L. Hall

Published in: Journal of Cardiovascular Translational Research | Issue 3/2015

Abstract

Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.

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Title: Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research
Authors: Neeta Adhikari
Kadambari Chandra Shekar
Rodney Staggs
Zaw Win
Kerianne Steucke
Yi-Wei Lin
Li-Na Wei
Patrick Alford
Jennifer L. Hall
Publication date: 01-04-2015
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 3/2015
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-015-9616-6

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Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

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