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01-09-2015 | Original Article

Glutamate receptors of the delta family are widely expressed in the adult brain

Authors: Régine Hepp, Y. Audrey Hay, Carolina Aguado, Rafael Lujan, Luce Dauphinot, Marie Claude Potier, Shinobu Nomura, Odile Poirel, Salah El Mestikawy, Bertrand Lambolez, Ludovic Tricoire

Published in: Brain Structure and Function | Issue 5/2015

Abstract

Recent reports point to critical roles of glutamate receptor subunit delta2 (GluD2) at excitatory synapses and link GluD1 gene alteration to schizophrenia but the expression patterns of these subunits in the brain remain almost uncharacterized. We examined the distribution of GluD1–2 mRNAs and proteins in the adult rodent brain, focusing mainly on GluD1. In situ hybridization revealed widespread neuronal expression of the GluD1 mRNA, with higher levels occurring in several forebrain regions and lower levels in cerebellum. Quantitative RT-PCR assessed differential GluD1 expression in cortex and cerebellum, and revealed GluD2 expression in cortex, albeit at markedly lower level than in cerebellum. Likewise, a high GluD1/GluD2 mRNA ratio was observed in cortex and a low ratio in cerebellum. GluD1 and GluD2 mRNAs were co-expressed in single cortical and hippocampal neurons, with a large predominance of GluD1. Western blots using GluD1- and GluD2-specific antibodies showed expression of both subunits in various brain structures, but not in non-nervous tissues examined. Both delta subunits were upregulated during postnatal development. Widespread neuronal expression of the GluD1 protein was confirmed using immunohistochemistry. Examination at the electron microscopic level in the hippocampus revealed that GluD1 was mainly localized at postsynaptic density of excitatory synapses on pyramidal cells. Control experiments performed using mice carrying deletion of the GluD1- or the GluD2-encoding gene confirmed the specificity of the present mRNA and protein analyses. Our results support a role for the delta family of glutamate receptors at excitatory synapses in neuronal networks throughout the adult brain.

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Title: Glutamate receptors of the delta family are widely expressed in the adult brain
Authors: Régine Hepp
Y. Audrey Hay
Carolina Aguado
Rafael Lujan
Luce Dauphinot
Marie Claude Potier
Shinobu Nomura
Odile Poirel
Salah El Mestikawy
Bertrand Lambolez
Ludovic Tricoire
Publication date: 01-09-2015
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 5/2015
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-014-0827-4

At a glance: The STEP trials

Springer Medicine

Glutamate receptors of the delta family are widely expressed in the adult brain

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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