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Cancer Cell International
Published in: Cancer Cell International 1/2022

Open Access 01-12-2022 | Glioblastoma | Primary Research

Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma

Authors: Zhiying Lin, Zhu Zhang, Haojie Zheng, Haiyan Xu, Yajuan Wang, Chao Chen, Junlu Liu, Guozhong Yi, Zhiyong Li, Xiaoyan Wang, Guanglong Huang

Published in: Cancer Cell International | Issue 1/2022

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Abstract

Background

Compared with the proneural (PN) subtype of glioblastoma (GBM), the mesenchymal (MES) subtype is more invasive and immune evasive and is closely related to poor prognosis. Here, we used transcriptome data and experimental evidence to indicate that CUB domain-containing protein 1 (CDCP1) is a novel regulator that facilitates the transformation of PN-GBM to MES-GBM.

Methods

The mRNA expression data of CDCP1 in glioma were collected from the TCGA, CGGA and GEO databases, and in vitro experiments verified CDCP1 expression in glioma tissue samples. Independent prognostic analysis revealed the correlation of the CDCP1 expression level and patient survival. Bioinformatics analysis and experiments verified the biological function of CDCP1. Multivariate proportional hazards models and a PPI network were used to select key genes. A prognostic risk model for predicting the survival of glioma patients was constructed based on the selected genes.

Results

The results showed that the expression of CDCP1 increased with increasing tumor grade and that the overexpression of CDCP1 correlated with a poor prognosis. CDCP1 was highly expressed in MES-GBM but weakly expressed in PN-GBM. The risk model (considering CDCP1 combined with CD44 and ITGAM expression) could represent a tool for predicting survival and prognosis in glioma patients.

Conclusions

Our study indicates that CDCP1 plays an important role in facilitating the transformation of PN-GBM to MES-GBM.
Appendix
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Metadata
Title
Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma
Authors
Zhiying Lin
Zhu Zhang
Haojie Zheng
Haiyan Xu
Yajuan Wang
Chao Chen
Junlu Liu
Guozhong Yi
Zhiyong Li
Xiaoyan Wang
Guanglong Huang
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2022
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-021-02373-1

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