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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 28-06-2023 | Glaucoma | Basic Science

TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells

Authors: Wenxu Zheng, Focke Ziemssen, Daniela Suesskind, Bogomil Voykov, Sven Schnichels

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 1/2024

Abstract

Purpose

Mechanosensitive channels (MSCs) and primary cilium possess a possible relevance for the sensation of intraocular pressure (IOP). However, there is only limited data on their expression and localization in the ciliary body epithelium (CBE). The purpose of this study was to characterize the expression and localization of TRPP2 in a human non-pigmented ciliary epithelial cell (HNPCE) line.

Methods

The expression of the TRPP2 was studied by quantitative (q)RT-PCR and in situ hybridization in rat and human tissue. Protein expression and distribution were studied by western blot analysis, immunohistochemistry, and immunoelectron microscopy. Cellular location of TRPP2 was determined in rat and human CBE by immunofluorescence and immunoblot analysis. Electron microscopy studies were conducted to evaluate where and with substructure TRPP2 is localized in the HNPCE cell line.

Results

The expression of TRPP2 in rat and human non-pigmented ciliary epithelium was detected. TRPP2 was mainly located in nuclei, but also showed a punctate distribution pattern in the cytoplasm of HNPCE of the tissue and the cell line. In HNPCE cell culture, primary cilia did exhibit different length following serum starvation and hydrostatic pressure. TRPP2 was found to be colocalized with these cilia in HNPCE cells.

Conclusion

The expression of TRPP2 and the primary cilium in the CB may indicate a possible role, such as the sensing of hydrostatic pressure, for the regulation of IOP. Functional studies via patch clamp or pharmacological intervention have yet to clarify the relevance for the physiological situation or aqueous humor regulation.

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Title: TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells
Authors: Wenxu Zheng
Focke Ziemssen
Daniela Suesskind
Bogomil Voykov
Sven Schnichels
Publication date: 28-06-2023
Publisher: Springer Berlin Heidelberg
Keyword: Glaucoma
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 1/2024
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-023-06150-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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