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01-04-2012 | Short Communication

Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism

Authors: Bing-rong Zhou, Yang Xu, Di Wu, Felicia Permatasari, Ying-ying Gao, Dan Luo

Published in: Archives of Dermatological Research | Issue 3/2012

Abstract

This study was aimed to investigate the protective effects of ginsenoside Rg1 on 8-methoxypsoralen(8-MOP)/Ultraviolet A (UVA)-induced premature senescence in human fibroblasts, and the underlying mechanism. We established a stress-induced premature senescence model by 8-MOP/UVA irradiation. The aging condition was determined by histochemical staining of senescence-associated β-galactosidase (SA-β-gal). Relative telomere length was calculated by the ratio of the amount of telomere DNA versus single copy DNA by real-time polymerase chain reaction, and protein levels of p-P53, p21^WAF−1 and p16^INK−4a were estimated by Western blotting. Compared with the 8-MOP/UVA treatment group, we found that the irradiated fibroblasts pretreated with ginsenoside Rg1 demonstrated a decrease in the expression of SA-β-gal, a downregulation in the level of senescence-associated proteins, and a deceleration in telomere shortening. Taken together, these results suggest that ginsenoside Rg1 significantly antagonizes premature senescence induced by 8-MOP/UVA in fibroblasts.

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Title: Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism
Authors: Bing-rong Zhou
Yang Xu
Di Wu
Felicia Permatasari
Ying-ying Gao
Dan Luo
Publication date: 01-04-2012
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 3/2012
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-012-1221-9

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Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism

Abstract

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Abstract

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