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Clinical Oral Investigations

Published in:

01-06-2021 | Gingival Diseases | Original Article

Free amino acid composition of saliva in patients with healthy periodontium and periodontitis

Authors: Nur Balci, Şivge Kurgan, Ali Çekici, Tülin Çakır, Muhittin A. Serdar

Published in: Clinical Oral Investigations | Issue 6/2021

Abstract

Objectives

To identify and compare the free amino acids in the saliva of periodontitis patients and healthy individuals and to assess their levels in different periodontal disease types.

Materials and methods

There were three groups: healthy individuals (control (C); n = 20), Stage III Grade B generalized periodontitis (GP-B; n = 20), and Stage III Grade C generalized periodontitis (GP-C; n = 20). Clinical periodontal parameters were measured. Amino acid analysis of the saliva was accomplished by liquid chromatography–mass spectrometry (LC MS/MS), taking the mean concentration.

Results

Citrulline and carnosine concentrations were significantly higher in patients with periodontitis than in the control group (p < 0.017). Methionine, glutamic acid, and arginine showed significantly higher concentrations in GP-C, whereas proline and tryptophan showed higher concentrations in the GP-B group (p < 0.017). There was a significant correlation between methionine, citrulline, arginine, and carnosine and clinical periodontal parameters.

Conclusions

Our results demonstrate that periodontal status and disease type can result in variations in salivary amino acid (AA) content in correlation with clinical inflammatory signs. The significant correlation of methionine, citrulline, carnosine, and arginine with clinical parameters, regardless of systemic status, suggests that the levels of different salivary free AAs play roles in periodontitis.

Clinical relevance

Salivary free AAs may be suggested as a potential diagnostic compound in patients with periodontitis.

Clinical trial registration

ClinicalTrials.gov NCT04642716

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Title: Free amino acid composition of saliva in patients with healthy periodontium and periodontitis
Authors: Nur Balci
Şivge Kurgan
Ali Çekici
Tülin Çakır
Muhittin A. Serdar
Publication date: 01-06-2021
Publisher: Springer Berlin Heidelberg
Keywords: Gingival Diseases
Bleeding on Probing
Dental Plaque Index
Published in: Clinical Oral Investigations / Issue 6/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-021-03977-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Free amino acid composition of saliva in patients with healthy periodontium and periodontitis

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Clinical trial registration

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Clinical trial registration

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