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Published in: Comparative Clinical Pathology 6/2012

01-12-2012 | Original Article

Genotyping of bovine viral diarrhea viruses (BVDV) isolated from cattle in Sicily

Authors: Vincenza Cannella, Elisabetta Giudice, Sara Ciulli, Patrizia Di Marco, Giuseppa Purpari, Giuseppe Cascone, Annalisa Guercio

Published in: Comparative Clinical Pathology | Issue 6/2012

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Abstract

Bovine Viral Diarrhea–Mucosal Disease (BVD–MD) is a widely spread infectious disease that causes important economic losses in farms. Several epidemiological studies indicate a high genetic heterogeneity among Bovine Viral Diarrhea Virus (BVDV) strains circulating in Italy. The aim of this study was to investigate the genotypes of BVDV in Sicily, a region in the South of Italy. For this purpose, 17 BVDV strains collected from cattle breed in Sicily between 2005 and 2008 were genetically typed by sequencing of the 5′-untraslated region (5′-UTR) of the viral genome. In this study, phylogenetic analysis showed that all 17 examined strains were clustered within the BVDV genotype 1. Particularly, 14 of them were clustered with the BVDV-1b subgroup, while the remaining three strains were clustered with the BVDV-1e. Moreover, the restriction analysis indicated a bovine origin for all of the 17 strains typed in this study. These results could be useful to carry out an epidemiological survey and to create vaccines that protect cattle against BVDV different subgroups.
Literature
go back to reference Baker JC (1995) The clinical manifestation of bovine viral diarrhea infection. Vet Clin North Am Food Anim Proct 14:425–445 Baker JC (1995) The clinical manifestation of bovine viral diarrhea infection. Vet Clin North Am Food Anim Proct 14:425–445
go back to reference Bolin SR, Grooms DL (2004) Origination and consequences of bovine viral diarrhea virus diversity. Vet Clin North Am Food Anim Pract 20:51–68PubMedCrossRef Bolin SR, Grooms DL (2004) Origination and consequences of bovine viral diarrhea virus diversity. Vet Clin North Am Food Anim Pract 20:51–68PubMedCrossRef
go back to reference Ciulli S, Galletti E, Battilani M, Scagliarini A, Gentile A, Morganti L, Prosperi S (2008) Genetic typing of bovine diarrhoea virus: evidence of an increasing number of variants in Italy. New Microbiol 31:263–271PubMed Ciulli S, Galletti E, Battilani M, Scagliarini A, Gentile A, Morganti L, Prosperi S (2008) Genetic typing of bovine diarrhoea virus: evidence of an increasing number of variants in Italy. New Microbiol 31:263–271PubMed
go back to reference Collet MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the Pestivirus Bovine Viral Diarrhoea Virus. Virology 165:191–199CrossRef Collet MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the Pestivirus Bovine Viral Diarrhoea Virus. Virology 165:191–199CrossRef
go back to reference Corapi W, French T, Dubovi E (1989) Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhea virus. J Virol 63:3934–3943PubMed Corapi W, French T, Dubovi E (1989) Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhea virus. J Virol 63:3934–3943PubMed
go back to reference Couvreur B, Letellier C, Collard A, Quenon P, Dehan P, Hamers C, Pastoret P (2002) Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium. Virus Res 85:17–28PubMedCrossRef Couvreur B, Letellier C, Collard A, Quenon P, Dehan P, Hamers C, Pastoret P (2002) Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium. Virus Res 85:17–28PubMedCrossRef
go back to reference Deng R, Brock KV (1992) Molecular cloning and nucleotide sequence of a Pestivirus genome, noncytophatic bovine viral diarrhoea virus strain SD-1. Virology 191:867–879PubMedCrossRef Deng R, Brock KV (1992) Molecular cloning and nucleotide sequence of a Pestivirus genome, noncytophatic bovine viral diarrhoea virus strain SD-1. Virology 191:867–879PubMedCrossRef
go back to reference Di Marco V, Luzzago C, Guercio A, Purpari G, Priolo I, Zecconi A (2002) Sindrome emorragica in bovini infetti da diarrea virale bovina. LVI National Congress SISVET, Giardini Naxos, pp 191–192 Di Marco V, Luzzago C, Guercio A, Purpari G, Priolo I, Zecconi A (2002) Sindrome emorragica in bovini infetti da diarrea virale bovina. LVI National Congress SISVET, Giardini Naxos, pp 191–192
go back to reference Falcone E, Tollis M, Conti G (1999) Bovine viral diarrhea disease associated with a contaminated vaccine. Virology 18:387–388 Falcone E, Tollis M, Conti G (1999) Bovine viral diarrhea disease associated with a contaminated vaccine. Virology 18:387–388
go back to reference Falcone E, Cordioli P, Sala G, Tarantino M, Tollis M (2001) Genotyping of bovine diarrhoea viruses isolated from cattle in Northen Italy. Vet Res Commun 25:161–67PubMedCrossRef Falcone E, Cordioli P, Sala G, Tarantino M, Tollis M (2001) Genotyping of bovine diarrhoea viruses isolated from cattle in Northen Italy. Vet Res Commun 25:161–67PubMedCrossRef
go back to reference Falcone E, Cordioli P, Tarantino M, Muscillo M, La Rosa G, Tollis M (2003) Genetic heterogeneity of bovine diarrhoea virus in Italy. Vet Res Commun 27:485–494PubMedCrossRef Falcone E, Cordioli P, Tarantino M, Muscillo M, La Rosa G, Tollis M (2003) Genetic heterogeneity of bovine diarrhoea virus in Italy. Vet Res Commun 27:485–494PubMedCrossRef
go back to reference Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy, classification and nomenclature of viruses. Eighth ICTV Report, Academic Press, San Diego, California Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy, classification and nomenclature of viruses. Eighth ICTV Report, Academic Press, San Diego, California
go back to reference Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRef Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRef
go back to reference Felsenstein J (2001) Phylogenetic inference package 3.6 (alpha3). Distributed by the author, Department of Genetics University of Washington, Seattle Felsenstein J (2001) Phylogenetic inference package 3.6 (alpha3). Distributed by the author, Department of Genetics University of Washington, Seattle
go back to reference Ferrari G, Scicluna MT, Bonvicini D, Gobbi C, Della Verità F, Valentine A, Autorino GL (1999) Bovine virus diarrhoea (BVD) control programme in an area in the Rome province (Italy). Vet Microbiol 64:237–245PubMedCrossRef Ferrari G, Scicluna MT, Bonvicini D, Gobbi C, Della Verità F, Valentine A, Autorino GL (1999) Bovine virus diarrhoea (BVD) control programme in an area in the Rome province (Italy). Vet Microbiol 64:237–245PubMedCrossRef
go back to reference Fulton RW, Ridpath JF, Confer AW, Saliki JT, Burge LJ, Payton ME (2003) Bovine viral diarrhoea virus antigenic diversity: impact on disease and vaccination programmes. Biologicals 31:89–95PubMedCrossRef Fulton RW, Ridpath JF, Confer AW, Saliki JT, Burge LJ, Payton ME (2003) Bovine viral diarrhoea virus antigenic diversity: impact on disease and vaccination programmes. Biologicals 31:89–95PubMedCrossRef
go back to reference Giammarioli M, Pellegrini C, Casciari C, Rossi E, De Mia GM (2008) Genetic diversity of bovine viral diarrhea virus 1: Italian isolates clustered in at least seven subgenotypes. J Vet Diagn Invest 20:783–788PubMedCrossRef Giammarioli M, Pellegrini C, Casciari C, Rossi E, De Mia GM (2008) Genetic diversity of bovine viral diarrhea virus 1: Italian isolates clustered in at least seven subgenotypes. J Vet Diagn Invest 20:783–788PubMedCrossRef
go back to reference Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98 Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98
go back to reference Hamers C, Lecomte C, Kulcsar G, Lambot M, Pastoret PP (1998) Persistently infected cattle stabilise bovine viral diarrhea virus leading to herd specific strains. Vet Microbiol 61:177–182PubMedCrossRef Hamers C, Lecomte C, Kulcsar G, Lambot M, Pastoret PP (1998) Persistently infected cattle stabilise bovine viral diarrhea virus leading to herd specific strains. Vet Microbiol 61:177–182PubMedCrossRef
go back to reference Houe H (1999) Epidemiological features and economical importance of bovine diarrhea virus (BVDV) infections. Vet Microbiol 64:89–107PubMedCrossRef Houe H (1999) Epidemiological features and economical importance of bovine diarrhea virus (BVDV) infections. Vet Microbiol 64:89–107PubMedCrossRef
go back to reference Hornberg A, Fernandez SR, Vogl C, Vilcek S, Matt M, Fink M, Kofer J, Schopf K (2009) Genetic diversity of Pestivirus isolates in cattle from Western Austria. Vet Microbiol 135:205–213PubMedCrossRef Hornberg A, Fernandez SR, Vogl C, Vilcek S, Matt M, Fink M, Kofer J, Schopf K (2009) Genetic diversity of Pestivirus isolates in cattle from Western Austria. Vet Microbiol 135:205–213PubMedCrossRef
go back to reference Jackova A, Novackova M, Pelletier C, Audeval C, Gueneau E, Haffar A, Petit E, Rehby L, Vilcek S (2008) The extended genetic diversity of BVDV-I: typing of BVDV isolates from France. Vet Res Commun 32:7–11PubMedCrossRef Jackova A, Novackova M, Pelletier C, Audeval C, Gueneau E, Haffar A, Petit E, Rehby L, Vilcek S (2008) The extended genetic diversity of BVDV-I: typing of BVDV isolates from France. Vet Res Commun 32:7–11PubMedCrossRef
go back to reference Kim SG, Dubovi EJ (2003) A novel simple one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhoea virus in bulk milk, blood and follicular fluid samples. Biologicals 31:103–106PubMedCrossRef Kim SG, Dubovi EJ (2003) A novel simple one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhoea virus in bulk milk, blood and follicular fluid samples. Biologicals 31:103–106PubMedCrossRef
go back to reference Letellier C, Kerkhofs P, Wellemans G, Vanopdenbosh E (1999) Detection and genotyping of bovine viral diarrhoea virus strains by reverse transcription-polymerase chain amplification of the 5′ untraslated region. Vet Microbiol 64:155–167PubMedCrossRef Letellier C, Kerkhofs P, Wellemans G, Vanopdenbosh E (1999) Detection and genotyping of bovine viral diarrhoea virus strains by reverse transcription-polymerase chain amplification of the 5′ untraslated region. Vet Microbiol 64:155–167PubMedCrossRef
go back to reference Luzzago C, Piccinini R, Zepponi A, Zecconi A (1999) Study on prevalence of bovine viral diarrhoea virus (BVDV) antibodies in 29 Italian Dairy herds with reproductive problems. Vet Microbiol 64:247–252PubMedCrossRef Luzzago C, Piccinini R, Zepponi A, Zecconi A (1999) Study on prevalence of bovine viral diarrhoea virus (BVDV) antibodies in 29 Italian Dairy herds with reproductive problems. Vet Microbiol 64:247–252PubMedCrossRef
go back to reference Luzzago C, Bandi C, Bronzo V, Ruffo G, Zecconi A (2001) Distribution of bovine viral diarrhoea virus strains in intensive cattle herds in Italy. Vet Microbiol 83:265–274PubMedCrossRef Luzzago C, Bandi C, Bronzo V, Ruffo G, Zecconi A (2001) Distribution of bovine viral diarrhoea virus strains in intensive cattle herds in Italy. Vet Microbiol 83:265–274PubMedCrossRef
go back to reference Luzzago C, Frigerio M, Liandris E, Cesaris L, Cabrini L, Piccinini R, Zecconi A (2006) Piano di controllo volontario delle infezioni da BVD nelle province di Lecco e di Como: risultati del triennio 2002–2004. L'Osservatorio 1:4–8 Luzzago C, Frigerio M, Liandris E, Cesaris L, Cabrini L, Piccinini R, Zecconi A (2006) Piano di controllo volontario delle infezioni da BVD nelle province di Lecco e di Como: risultati del triennio 2002–2004. L'Osservatorio 1:4–8
go back to reference Luzzago C, Frigerio M, Piccinini R, Daprà V, Zecconi A (2008) A scoring system for risk assessment of the introduction and spread of bovine viral diarrhoea virus in dairy herds in Northern Italy. Vet J 177:236–241PubMedCrossRef Luzzago C, Frigerio M, Piccinini R, Daprà V, Zecconi A (2008) A scoring system for risk assessment of the introduction and spread of bovine viral diarrhoea virus in dairy herds in Northern Italy. Vet J 177:236–241PubMedCrossRef
go back to reference Martucciello A, De Mia GM, Giammarioli M, De Donato I, Iovane G, Galiero G (2009) Detection of bovine viral diarrhoea virus from three water buffalo fetuses (Bubalus bubalis) in southern Italy. J Vet Diagn Invest 21:137–140PubMedCrossRef Martucciello A, De Mia GM, Giammarioli M, De Donato I, Iovane G, Galiero G (2009) Detection of bovine viral diarrhoea virus from three water buffalo fetuses (Bubalus bubalis) in southern Italy. J Vet Diagn Invest 21:137–140PubMedCrossRef
go back to reference Mishra N, Rajukumar K, Vilceck S, Tiwari A, Satav JS, Dubey SC (2008) Molecular charcterization of bovine viral diarrhoea virus type 2 isolate originating from a native Indian sheep (Ovies aries). Vet Microbiol 130:88–98PubMedCrossRef Mishra N, Rajukumar K, Vilceck S, Tiwari A, Satav JS, Dubey SC (2008) Molecular charcterization of bovine viral diarrhoea virus type 2 isolate originating from a native Indian sheep (Ovies aries). Vet Microbiol 130:88–98PubMedCrossRef
go back to reference Nagai M, Ito T, Sugita S, Genno A, Takeuchi K, Ozawa T, Sakoda Y, Nishimori T, Takamura K, Akashi H (2001) Genomic and serological diversity of bovine viral diarrhea virus in Japan. Arch Virol 146:685–696PubMedCrossRef Nagai M, Ito T, Sugita S, Genno A, Takeuchi K, Ozawa T, Sakoda Y, Nishimori T, Takamura K, Akashi H (2001) Genomic and serological diversity of bovine viral diarrhea virus in Japan. Arch Virol 146:685–696PubMedCrossRef
go back to reference Nagai M, Hayashi M, Itou M, Fukutomi T, Akashi H, Kida H, Sakoda Y (2008) Identification of new genetic subtypes of bovine viral diarrhoea virus genotype I isolated in Japan. Virus Gen 36:135–139CrossRef Nagai M, Hayashi M, Itou M, Fukutomi T, Akashi H, Kida H, Sakoda Y (2008) Identification of new genetic subtypes of bovine viral diarrhoea virus genotype I isolated in Japan. Virus Gen 36:135–139CrossRef
go back to reference Obritzhauser W, Klemens F, Josef K (2005) BVDV infection risk in the course of a voluntary BVDV eradication program in Styria/Austria. Pre Vet Med 72:127–132CrossRef Obritzhauser W, Klemens F, Josef K (2005) BVDV infection risk in the course of a voluntary BVDV eradication program in Styria/Austria. Pre Vet Med 72:127–132CrossRef
go back to reference Perdrizet JA, Rebhun WC, Dubovi EJ, Donis RO (1987) Bovine virus diarrhea—clinical syndromes in dairy herds. Cornell Vet 77:46–74PubMed Perdrizet JA, Rebhun WC, Dubovi EJ, Donis RO (1987) Bovine virus diarrhea—clinical syndromes in dairy herds. Cornell Vet 77:46–74PubMed
go back to reference Purpari G, Alimena G, Di Marco P, Cascone G, Tumino G, Guercio A (2005) Valutazione della prevalenza di IBR e BVD nella regione Sicilia: un modello sperimentale. XXXVII Buiatric National Congress. Purpari G, Alimena G, Di Marco P, Cascone G, Tumino G, Guercio A (2005) Valutazione della prevalenza di IBR e BVD nella regione Sicilia: un modello sperimentale. XXXVII Buiatric National Congress.
go back to reference Pratelli A, Martella V, Cirone F, Buonavoglia D, Elia G, Tempesta M, Buonavoglia C (2001) Genomic characterization of Pestiviruses isolated from lambs and kids in southern Italy. J Virol Met 94:81–85CrossRef Pratelli A, Martella V, Cirone F, Buonavoglia D, Elia G, Tempesta M, Buonavoglia C (2001) Genomic characterization of Pestiviruses isolated from lambs and kids in southern Italy. J Virol Met 94:81–85CrossRef
go back to reference Ridpath JF, Bolin SR, Dubovi EJ (1994) Segregation of bovine diarrhoea virus into genotypes. Virology 205:66–74PubMedCrossRef Ridpath JF, Bolin SR, Dubovi EJ (1994) Segregation of bovine diarrhoea virus into genotypes. Virology 205:66–74PubMedCrossRef
go back to reference Ridpath JF, Neill JD (2000) Detection and characterization of genetic recombination in cytopathic type 2 bovine viral diarrhea viruses. J Virol 74:8771–8774PubMedCrossRef Ridpath JF, Neill JD (2000) Detection and characterization of genetic recombination in cytopathic type 2 bovine viral diarrhea viruses. J Virol 74:8771–8774PubMedCrossRef
go back to reference Tajima M, Frey HR, Yamato O, Maede Y, Moenning V, Scholz H, Greiser-Wilke I (2001) Prevalence of genotypes 1 and 2 of bovine viral diarrhea virus in Lower Saxony, Germany. Virus Res 76:31–42PubMedCrossRef Tajima M, Frey HR, Yamato O, Maede Y, Moenning V, Scholz H, Greiser-Wilke I (2001) Prevalence of genotypes 1 and 2 of bovine viral diarrhea virus in Lower Saxony, Germany. Virus Res 76:31–42PubMedCrossRef
go back to reference Thompson JD, Higgins GD, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucleic Acid Res 22:4673–4680PubMedCrossRef Thompson JD, Higgins GD, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucleic Acid Res 22:4673–4680PubMedCrossRef
go back to reference Vilcek S, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136:309–323PubMedCrossRef Vilcek S, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136:309–323PubMedCrossRef
go back to reference Vilcek S, Paton DJ, Durkovic B, Strojny L, Ibata G, Moussa A, Loitsch A, Rossmanith W, Vega S, Scicluna MT, Palfi V (2001) Bovine diarrhoea virus genotype 1 can be separated into at least eleven genetic groups. Arch Virol 146:99–115PubMedCrossRef Vilcek S, Paton DJ, Durkovic B, Strojny L, Ibata G, Moussa A, Loitsch A, Rossmanith W, Vega S, Scicluna MT, Palfi V (2001) Bovine diarrhoea virus genotype 1 can be separated into at least eleven genetic groups. Arch Virol 146:99–115PubMedCrossRef
go back to reference Vilcek S, Durkovic B, Bobakova M, Sharp G, Paton DJ (2002) Identification of bovine diarrhea virus 2 in cattle in Slovakia. Vet Rec 151:150–152PubMedCrossRef Vilcek S, Durkovic B, Bobakova M, Sharp G, Paton DJ (2002) Identification of bovine diarrhea virus 2 in cattle in Slovakia. Vet Rec 151:150–152PubMedCrossRef
go back to reference Vilcek S, Durkovic B, Kolesarova M, Greiser-Wilke I, Paton D (2004) Genetic diversity of international bovine viral diarrhoea virus (BVDV) isolates: identification of a new BVDV-1 genetic group. Vet Res 35:609–615PubMedCrossRef Vilcek S, Durkovic B, Kolesarova M, Greiser-Wilke I, Paton D (2004) Genetic diversity of international bovine viral diarrhoea virus (BVDV) isolates: identification of a new BVDV-1 genetic group. Vet Res 35:609–615PubMedCrossRef
go back to reference Yesilbag K, Forster C, Bank-Wolf B, Ylmaz Z, Alcan F, Ozkul A, Burgu I, Rosales SC, Thiel HJ, Konig M (2008) Genetic heterogeneity of bovine viral diarrhea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1. Vet Microbiol 130:258–267CrossRef Yesilbag K, Forster C, Bank-Wolf B, Ylmaz Z, Alcan F, Ozkul A, Burgu I, Rosales SC, Thiel HJ, Konig M (2008) Genetic heterogeneity of bovine viral diarrhea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1. Vet Microbiol 130:258–267CrossRef
go back to reference Zhu LQ, Ren M, Lin YQ, Ding XY, Zhang GP, Zhao X, Zhu GQ (2009) Identification of a bovine viral diarrhea virus 2 isolated from cattle in China. Acta Virol 53:131–134PubMedCrossRef Zhu LQ, Ren M, Lin YQ, Ding XY, Zhang GP, Zhao X, Zhu GQ (2009) Identification of a bovine viral diarrhea virus 2 isolated from cattle in China. Acta Virol 53:131–134PubMedCrossRef
Metadata
Title
Genotyping of bovine viral diarrhea viruses (BVDV) isolated from cattle in Sicily
Authors
Vincenza Cannella
Elisabetta Giudice
Sara Ciulli
Patrizia Di Marco
Giuseppa Purpari
Giuseppe Cascone
Annalisa Guercio
Publication date
01-12-2012
Publisher
Springer-Verlag
Published in
Comparative Clinical Pathology / Issue 6/2012
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-011-1358-5

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