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Tumor Biology
Published in: Tumor Biology 5/2014

01-05-2014 | Research Article

Genomic characterization of three urinary bladder cancer cell lines: understanding genomic types of urinary bladder cancer

Authors: Rosário Pinto-Leite, Isabel Carreira, Joana Melo, Susana Isabel Ferreira, Ilda Ribeiro, Jaqueline Ferreira, Marco Filipe, Carina Bernardo, Regina Arantes-Rodrigues, Paula Oliveira, Lúcio Santos

Published in: Tumor Biology | Issue 5/2014

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Abstract

Several genomic regions are frequently altered and associated with the type, stage and progression of urinary bladder cancer (UBC). We present the characterization of 5637, T24 and HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative genomic hybridization (aCGH) and multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies present in UBC were found in the three cell lines, such as chromosome 20 aneuploidy and the loss of 9p21. Some gene loci losses (e.g. CDKN2A) and gains (e.g. HRAS, BCL2L1 and PTPN1) were coincident across all cell lines. Although some significant heterogeneity and complexity were detected between them, their genomic profiles exhibited a similar pattern to UBC. We suggest that 5637 and HT1376 represent the E2F3/RB1 pathway due to amplification of 6p22.3, concomitant with loss of one copy of RB1 and mutation of the remaining copy. The HT1376 presented a 10q deletion involving PTEN region and no alteration of PIK3CA region which, in combination with the inactivation of TP53, bears more invasive and metastatic properties than 5637. The T24 belongs to the alternative pathway of FGFR3/CCND1 by presenting mutated HRAS and over-represented CCND1. These cell lines cover the more frequent subtypes of UBC and are reliable models that can be used, as a group, in preclinical studies.
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Metadata
Title
Genomic characterization of three urinary bladder cancer cell lines: understanding genomic types of urinary bladder cancer
Authors
Rosário Pinto-Leite
Isabel Carreira
Joana Melo
Susana Isabel Ferreira
Ilda Ribeiro
Jaqueline Ferreira
Marco Filipe
Carina Bernardo
Regina Arantes-Rodrigues
Paula Oliveira
Lúcio Santos
Publication date
01-05-2014
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 5/2014
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-013-1604-3

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