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01-04-2008 | Original Article

Genetic variations in the gB, UL144 and UL149 genes of human cytomegalovirus strains collected from congenitally and postnatally infected Japanese children

Authors: Hainian Yan, Shin Koyano, Yuhki Inami, Yumiko Yamamoto, Tatsuo Suzutani, Masashi Mizuguchi, Hiroshi Ushijima, Ichiro Kurane, Naoki Inoue

Published in: Archives of Virology | Issue 4/2008

Abstract

Human cytomegalovirus (CMV) is the leading cause of intrauterine viral infection. The association of genetic polymorphisms in some particular genes with the incidence and severity of congenital infection has been controversial. To address this issue, we analyzed the genotypes of the glycoprotein B (gB), UL144 and UL149 genes of CMV clinical strains obtained from 33 congenitally and 31 postnatally infected Japanese children. Our results demonstrated that (1) CMV strains with any combination of genotypes could be vertically transmitted from mother to fetus, potentially causing neurological abnormalities, (2) the gB3 genotype was more prevalent in the congenital cases than in postnatally infected children (P < 0.05), particularly in congenital cases with sensorineural hearing loss (P = 0.009), (3) there was no relationship between gB genotype and viral load in the urine and dried umbilical cord specimens in the congenital cases, and (4) the UL144 and UL149 genotype distributions had no bias for congenial infection. In future studies, it would be interesting to see whether the gB genotypes serve as a prognostic indicator of CMV-associated diseases.

Aquino VH, Figueiredo LT (2000) High prevalence of renal transplant recipients infected with more than one cytomegalovirus glycoprotein B genotype. J Med Virol 61(1):138–142PubMedCrossRef

Arav-Boger R, Battaglia CA, Lazzarotto T, Gabrielli L, Zong JC, Hayward GS, Diener-West M, Landini MP (2006) Cytomegalovirus (CMV)-encoded UL144 (truncated tumor necrosis factor receptor) and outcome of congenital CMV infection. J Infect Dis 194(4):464–473PubMedCrossRef

Arav-Boger R, Foster CB, Zong JC, Pass RF (2006) Human cytomegalovirus-encoded alpha -chemokines exhibit high sequence variability in congenitally infected newborns. J Infect Dis 193(6):788–791PubMedCrossRef

Arav-Boger R, Willoughby RE, Pass RF, Zong JC, Jang WJ, Alcendor D, Hayward GS (2002) Polymorphisms of the cytomegalovirus (CMV)-encoded tumor necrosis factor-alpha and beta-chemokine receptors in congenital CMV disease. J Infect Dis 186(8):1057–1064PubMedCrossRef

Bale JF Jr, Murph JR, Demmler GJ, Dawson J, Miller JE, Petheram SJ (2000) Intrauterine cytomegalovirus infection and glycoprotein B genotypes. J Infect Dis 182(3):933–936PubMedCrossRef

Bale JF Jr, Petheram SJ, Robertson M, Murph JR, Demmler G (2001) Human cytomegalovirus a sequence and UL144 variability in strains from infected children. J Med Virol 65(1):90–96PubMedCrossRef

Barbi M, Binda S, Caroppo S, Primache V, Dido P, Guidotti P, Corbetta C, Melotti D (2001) CMV gB genotypes and outcome of vertical transmission: study on dried blood spots of congenitally infected babies. J Clin Virol 21(1):75–79PubMedCrossRef

Bongarts A, Von Laer D, Vogelberg C, Ebert K, Van Lunzen J, Garweg J, Vaith P, Hufert FT, Haller O, Meyer-Konig U (1996) Glycoprotein B genotype of human cytomegalovirus: distribution in HIV-infected patients. Scand J Infect Dis 28(5):447–449PubMedCrossRef

Boppana SB, Fowler KB, Pass RF, Rivera LB, Bradford RD, Lakeman FD, Britt WJ (2005) Congenital cytomegalovirus infection: association between virus burden in infancy and hearing loss. J Pediatr 146(6):817–823PubMedCrossRef

10.

Boppana SB, Rivera LB, Fowler KB, Mach M, Britt WJ (2001) Intrauterine transmission of cytomegalovirus to infants of women with preconceptional immunity. N Engl J Med 344(18):1366–1371PubMedCrossRef

11.

Chou SW (1992) Comparative analysis of sequence variation in gp116 and gp55 components of glycoprotein B of human cytomegalovirus. Virology 188(1):388–390PubMedCrossRef

12.

Chou SW, Dennison KM (1991) Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes. J Infect Dis 163(6):1229–1234PubMed

13.

Coaquette A, Bourgeois A, Dirand C, Varin A, Chen W, Herbein G (2004) Mixed cytomegalovirus glycoprotein B genotypes in immunocompromised patients. Clin Infect Dis 39(2):155–161PubMedCrossRef

14.

Dolan A, Cunningham C, Hector RD, Hassan-Walker AF, Lee L, Addison C, Dargan DJ, McGeoch DJ, Gatherer D, Emery VC, Griffiths PD, Sinzger C, McSharry BP, Wilkinson GW, Davison AJ (2004) Genetic content of wild-type human cytomegalovirus. J Gen Virol 85(Pt 5):1301–1312PubMedCrossRef

15.

Fries BC, Chou S, Boeckh M, Torok-Storb B (1994) Frequency distribution of cytomegalovirus envelope glycoprotein genotypes in bone marrow transplant recipients. J Infect Dis 169(4):769–774PubMed

16.

Gaytant MA, Steegers EA, Semmekrot BA, Merkus HM, Galama JM (2002) Congenital cytomegalovirus infection: review of the epidemiology and outcome. Obstet Gynecol Surv 57(4):245–256PubMedCrossRef

17.

Hahn G, Revello MG, Patrone M, Percivalle E, Campanini G, Sarasini A, Wagner M, Gallina A, Milanesi G, Koszinowski U, Baldanti F, Gerna G (2004) Human cytomegalovirus UL131–128 genes are indispensable for virus growth in endothelial cells and virus transfer to leukocytes. J Virol 78(18):10023–10033PubMedCrossRef

18.

Humar A, Kumar D, Gilbert C, Boivin G (2003) Cytomegalovirus (CMV) glycoprotein B genotypes and response to antiviral therapy, in solid-organ-transplant recipients with CMV disease. J Infect Dis 188(4):581–584PubMedCrossRef

19.

Inoue N, Koyano S (2008) Evaluation of screening tests for congenital cytomegalovirus infection. Ped Infec Dis J 27(2):182–184

20.

Ishibashi K, Tokumoto T, Tanabe K, Shirakawa H, Hashimoto K, Kushida N, Yanagida T, Inoue N, Yamaguchi O, Toma H, Suzutani T (2007) Association of the outcome of renal transplantation with antibody response to cytomegalovirus strain-specific glycoprotein H epitopes. Clin Infect Dis 45(1):60–67PubMedCrossRef

21.

Ji YH, Ruan Q, Sun ZR, Ma YP, He R, Qi Y, Huang YJ (2006) Structure and variability of the UL149 open reading frame from low-passage clinical isolates of human cytomegalovirus. J Virol Methods 131(1):72–77PubMedCrossRef

22.

Jin H, Wang X, Li S (2007) Human cytomegalovirus glycoprotein B genotype correlates with different symptoms of infected infants. Intervirology 50(3):219–223PubMedCrossRef

23.

Koyano S, Araki A, Hirano Y, Fujieda K, Suzutani T, Yagyu K, Murono K, Inoue N (2004) Retrospective diagnosis of congenital cytomegalovirus infection using dried umbilical cords. Pediatr Infect Dis J 23(5):481–482PubMedCrossRef

24.

Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5(2):150–163PubMedCrossRef

25.

Lurain NS, Kapell KS, Huang DD, Short JA, Paintsil J, Winkfield E, Benedict CA, Ware CF, Bremer JW (1999) Human cytomegalovirus UL144 open reading frame: sequence hypervariability in low-passage clinical isolates. J Virol 73(12):10040–10050PubMed

26.

Mao ZQ, He R, Sun M, Qi Y, Huang YJ, Ruan Q (2007) The relationship between polymorphisms of HCMV UL144 ORF and clinical manifestations in 73 strains with congenital and/or perinatal HCMV infection. Arch Virol 152(1):115–124PubMedCrossRef

27.

Mattick C, Dewin D, Polley S, Sevilla-Reyes E, Pignatelli S, Rawlinson W, Wilkinson G, Dal Monte P, Gompels UA (2004) Linkage of human cytomegalovirus glycoprotein gO variant groups identified from worldwide clinical isolates with gN genotypes, implications for disease associations and evidence for N-terminal sites of positive selection. Virology 318(2):582–597PubMedCrossRef

28.

Meyer-König U, Vogelberg C, Bongarts A, Kampa D, Delbruck R, Wolff-Vorbeck G, Kirste G, Haberland M, Hufert FT, von Laer D (1998) Glycoprotein B genotype correlates with cell tropism in vivo of human cytomegalovirus infection. J Med Virol 55(1):75–81PubMedCrossRef

29.

Murayama T, Takegoshi M, Tanuma J, Eizuru Y (2005) Analysis of human cytomegalovirus UL144 variability in low-passage clinical isolates in Japan. Intervirology 48(2–3):201–206PubMedCrossRef

30.

Ogawa H, Baba Y, Suzutani T, Inoue N, Fukushima E, Omori K (2006) Congenital cytomegalovirus infection diagnosed by polymerase chain reaction with the use of preserved umbilical cord in sensorineural hearing loss children. Laryngoscope 116(11):1991–1994PubMedCrossRef

31.

Ogawa H, Suzutani T, Baba Y, Koyano S, Nozawa N, Ishibashi K, Fujieda K, Inoue N, Omori K (2007) Etiology of severe sensorineural hearing loss in children: independent impact of congenital cytomegalovirus infection and GJB2 mutations. J Infect Dis 195(6):782–788PubMedCrossRef

32.

Pass RF (2001) Cytomegalovirus. In: Knipe DM, Howley PM (eds) Fields virology, 4th edn. Lippincott Williams & Wilkins, Philadelphia, pp 2675–2705

33.

Peek R, Verbraak F, Bruinenberg M, Van der LA, Van den HG, Kijlstra A (1998) Cytomegalovirus glycoprotein B genotyping in ocular fluids and blood of AIDS patients with cytomegalovirus retinitis. Invest Ophthalmol Vis Sci 39(7):1183–1187PubMed

34.

Picone O, Costa JM, Chaix ML, Ville Y, Rouzioux C, Leruez-Ville M (2005) Human cytomegalovirus UL144 gene polymorphisms in congenital infections. J Clin Microbiol 43(1):25–29PubMedCrossRef

35.

Picone O, Costa JM, Leruez-Ville M, Ernault P, Olivi M, Ville Y (2004) Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses. Prenat Diagn 24(12):1001–1006PubMedCrossRef

36.

Pignatelli S, Dal Monte P, Rossini G, Lazzarotto T, Gatto MR, Landini MP (2003) Intrauterine cytomegalovirus infection and glycoprotein N (gN) genotypes. J Clin Virol 28(1):38–43PubMedCrossRef

37.

Pignatelli S, Dal Monte P, Rossini G, Landini MP (2004) Genetic polymorphisms among human cytomegalovirus (HCMV) wild-type strains. Rev Med Virol 14(6):383–410PubMedCrossRef

38.

Prichard MN, Penfold ME, Duke GM, Spaete RR, Kemble GW (2001) A review of genetic differences between limited and extensively passaged human cytomegalovirus strains. Rev Med Virol 11(3):191–200PubMedCrossRef

39.

Puchhammer-Stockl E, Gorzer I (2006) Cytomegalovirus and Epstein-Barr virus subtypes—the search for clinical significance. J Clin Virol 36(4):239–248PubMedCrossRef

40.

Puchhammer-Stockl E, Gorzer I, Zoufaly A, Jaksch P, Bauer CC, Klepetko W, Popow-Kraupp T (2006) Emergence of multiple cytomegalovirus strains in blood and lung of lung transplant recipients. Transplantation 81(2):187–194PubMedCrossRef

41.

Rasmussen L (1999) Molecular pathogenesis of human cytomegalovirus infection. Transpl Infect Dis 1(2):127–134PubMedCrossRef

42.

Rasmussen L, Geissler A, Winters M (2003) Inter- and intragenic variations complicate the molecular epidemiology of human cytomegalovirus. J Infect Dis 187(5):809–819PubMedCrossRef

43.

Sarcinella L, Mazzulli T, Willey B, Humar A (2002) Cytomegalovirus glycoprotein B genotype does not correlate with outcomes in liver transplant patients. J Clin Virol 24(1–2):99–105PubMedCrossRef

44.

Shepp DH, Match ME, Lipson SM, Pergolizzi RG (1998) A fifth human cytomegalovirus glycoprotein B genotype. Res Virol 149(2):109–114PubMedCrossRef

45.

Stanton R, Westmoreland D, Fox JD, Davison AJ, Wilkinson GW (2005) Stability of human cytomegalovirus genotypes in persistently infected renal transplant recipients. J Med Virol 75(1):42–46PubMedCrossRef

46.

Tanaka K, Numazaki K, Tsutsumi H (2005) Human cytomegalovirus genetic variability in strains isolated from Japanese children during 1983–2003. J Med Virol 76(3):356–360PubMedCrossRef

47.

Torok-Storb B, Boeckh M, Hoy C, Leisenring W, Myerson D, Gooley T (1997) Association of specific cytomegalovirus genotypes with death from myelosuppression after marrow transplantation. Blood 90(5):2097–2102PubMed

48.

Trincado DE, Scott GM, White PA, Hunt C, Rasmussen L, Rawlinson WD (2000) Human cytomegalovirus strains associated with congenital and perinatal infections. J Med Virol 61(4):481–487PubMedCrossRef

49.

Vilas Boas LS, de Souza VA, Penalva de Oliveira AC, Rodriguez Viso AT, Nascimento Filho AM, Nascimento MC, Pannuti CS (2003) Cytomegalovirus glycoprotein B genotypes and central nervous system disease in AIDS patients. J Med Virol 71(3):404–407PubMedCrossRef

50.

Yu ZS, Zou CC, Zheng JY, Zhao ZY (2006) Cytomegalovirus gB genotype and clinical features in Chinese infants with congenital infections. Intervirology 49(5):281–285PubMedCrossRef

51.

Zipeto D, Hong C, Gerna G, Zavattoni M, Katzenstein D, Merigan TC, Rasmussen L (1998) Geographic and demographic differences in the frequency of human cytomegalovirus gB genotypes 1–4 in immunocompromised patients. AIDS Res Hum Retroviruses 14(6):533–536PubMedCrossRef

Title: Genetic variations in the gB, UL144 and UL149 genes of human cytomegalovirus strains collected from congenitally and postnatally infected Japanese children
Authors: Hainian Yan
Shin Koyano
Yuhki Inami
Yumiko Yamamoto
Tatsuo Suzutani
Masashi Mizuguchi
Hiroshi Ushijima
Ichiro Kurane
Naoki Inoue
Publication date: 01-04-2008
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-008-0044-7

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