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European Journal of Medical Research

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Open Access 01-12-2014 | Research

Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancer

Authors: Hongyan Zhang, Xuwei Wu, Yi Xiao, Mei Chen, Zhidong Li, Xing Wei, Kaifa Tang

Published in: European Journal of Medical Research | Issue 1/2014

Abstract

Background

Our objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes (GSTM1 and GSTT1) and evaluate oxidative damage in patients with non-small lung cancer (N-SCLC).

Methods

One hundred and ten patients with N-SCLC and 100 controls are included in this case-control study. Multiplex polymerase chain reaction (PCR) analyses were used to identify the genotypes. The activities of malondialdehyde (MDA) and nitric oxide (NO) and total antioxidant capacity (T-AOC) were detected by spectroscopic analysis.

Results

The frequencies of the GSTM1, T1, and GSTM1/T1 null genotypes in the patient group were significantly higher than that in the control group (OR = 2.071, P = 0.009; OR = 1.900, P = 0.024; OR = 3.258, P = 0.003). The activities of MDA and NO were significantly higher in the patient group than that in the control group (P <0.001), and T-AOC was significantly lower in patient group than that in control group (P <0.001). The activities of MDA, and NO were higher but the T-AOC was lower in patients with the GSTM1, T1 and M1/T1 null genotypes than those in patients with GSTM1, T1 and M1/T1 present genotypes (P <0.001).

Conclusions

Our results suggest that oxidative damage may be play a important role in patients with N-SCLC, and that GSTM1 and GSTT1 null genotypes may predispose the cells of patients with N-SCLC to increased oxidative damage.

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Thun MJ, Hannan LM, Adams-Campbell LL, Boffetta P, Buring JE, Feskanich D, Flanders WD, Jee SH, Katanoda K, Kolonel LN, Lee IM, Marugame T, Palmer JR, Riboli E, Sobue T, Avila-Tang E, Wilkens LR, Samet JM: Lung cancer occurrence in never-smokers: an analysis of 13 cohorts and 22 cancer registry studies. PLoS Med 2008, 5: e185. 10.1371/journal.pmed.0050185PubMedPubMedCentralCrossRef

Jemal A, Siegel R, Xu J, Ward E: Cancer statistics, 2010. CA Cancer J Clin 2010, 60: 277–300. 10.3322/caac.20073PubMedCrossRef

Wu W, Platoshyn O, Firth AL, Yuan JX: Hypoxia divergently regulates production of reactive oxygen species in human pulmonary and coronary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 2007, 293: L952–959. 10.1152/ajplung.00203.2007PubMedCrossRef

Kinnula VL, Crapo JD: Superoxide dismutases in the lung and human lung diseases. Am J Respir Crit Care Med 2003, 167: 1600–1619. 10.1164/rccm.200212-1479SOPubMedCrossRef

Farinati F, Piciocchi M, Lavezzo E, Bortolami M, Cardin R: Oxidative stress and inducible nitric oxide synthase induction in carcinogenesis. Dig Dis 2010, 28: 579–584. 10.1159/000320052PubMedCrossRef

Cadet J, Douki T, Ravanat JL: Oxidatively generated base damage to cellular DNA. Free Radic Biol Med 2010, 49: 9–21. 10.1016/j.freeradbiomed.2010.03.025PubMedCrossRef

Hair JM, Terzoudi GI, Hatzi VI, Lehockey KA, Srivastava D, Wang W, Pantelias GE, Georgakilas AG: BRCA1 role in the mitigation of radiotoxicity and chromosomal instability through repair of clustered DNA lesions. Chem Biol Interact 2010, 188: 350–358. 10.1016/j.cbi.2010.03.046PubMedCrossRef

Ito K, Yano T, Morodomi Y, Yoshida T, Kohno M, Haro A, Shikada Y, Okamoto T, Maruyama R, Maehara Y: Serum Antioxidant Capacity and Oxidative Injury to Pulmonary DNA in Never-smokers with Primary Lung Cancer. Anticancer Res 2012, 32: 1063–1067.PubMed

Peddireddy V, Badabagni SP, Gundimeda SD, Penagaluru PR, Mundluru HP: Assessment of 8-oxo-7, 8-dihydro-2′-deoxyguanosine and malondialdehyde levels as oxidative stress markers and antioxidant status in non-small cell lung cancer. Biomarkers 2012, 17: 261–268. 10.3109/1354750X.2012.664169PubMedCrossRef

10.

Strange RC, Spiteri MA, Ramachandran S, Fryer AA: Glutathione-S-transferase family of enzymes. Mutat Res 2001, 482: 21–26. 10.1016/S0027-5107(01)00206-8PubMedCrossRef

11.

Nebert DW, Vasiliou V: Analysis of the glutathione S-transferase (GST) gene family. Hum Genomics 2004, 1: 460–464. 10.1186/1479-7364-1-6-460PubMedPubMedCentralCrossRef

12.

Seidegard J, Ekstrom G: The role of human glutathione transferases and epoxide hydrolases in the metabolism of xenobiotics. Environ Health Perspect 1997, 105(Suppl 4):791–799. 10.1289/ehp.97105s4791PubMedPubMedCentralCrossRef

13.

Eaton DL, Bammler TK: Concise review of the glutathione S-transferases and their significance to toxicology. Toxicol Sci 1999, 49: 156–164. 10.1093/toxsci/49.2.156PubMedCrossRef

14.

Pemble S, Schroeder KR, Spencer SR, Meyer DJ, Hallier E, Bolt HM, Ketterer B, Taylor JB: Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem J 1994, 300(Pt 1):271–276.PubMedPubMedCentralCrossRef

15.

Pinarbasi H, Silig Y, Cetinkaya O, Seyfikli Z, Pinarbasi E: Strong association between the GSTM1-null genotype and lung cancer in a Turkish population. Cancer Genet Cytogenet 2003, 146: 125–129. 10.1016/S0165-4608(03)00059-1PubMedCrossRef

16.

Matakova T, Sivonova M, Halasova E, Mistuna D, Dzian A, Berzinec P, Letkova L, Dobrota D: Gene polymorphisms of biotransforming enzymes (GSTs) and their association with lung cancer in the Slovakian population. Eur J Med Res 2009, 14(Suppl 4):275–279. 10.1186/2047-783X-14-S4-275PubMedPubMedCentralCrossRef

17.

Tamaki Y, Arai T, Sugimura H, Sasaki T, Honda M, Muroi Y, Matsubara Y, Kanno S, Ishikawa M, Hirasawa N, Hiratsuka M: Association between cancer risk and drug-metabolizing enzyme gene (CYP2A6, CYP2A13, CYP4B1, SULT1A1, GSTM1, and GSTT1) polymorphisms in cases of lung cancer in Japan. Drug Metab Pharmacokinet 2011, 26: 516–522. 10.2133/dmpk.DMPK-11-RG-046PubMedCrossRef

18.

To-Figueras J, Gene M, Gomez-Catalan J, Galan MC, Fuentes M, Ramon JM, Rodamilans M, Huguet E, Corbella J: Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) polymorphisms and lung cancer risk among Northwestern Mediterraneans. Carcinogenesis 1997, 18: 1529–1533. 10.1093/carcin/18.8.1529PubMedCrossRef

19.

Tang K, Xue W, Xing Y, Xu S, Wu Q, Liu R, Wang X, Xing J: Genetic polymorphisms of glutathione s-transferase M1, T1, and P1, and the assessment of oxidative damage in infertile men with varicoceles from northwestern china. J Androl 2012, 33: 257–263. 10.2164/jandrol.110.012468PubMedCrossRef

20.

Durackova Z: Some current insights into oxidative stress. Physiol Res 2010, 59: 459–469.PubMed

21.

Visconti R, Grieco D: New insights on oxidative stress in cancer. Curr Opin Drug Discov Devel 2009, 12: 240–245.PubMed

Title: Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancer
Authors: Hongyan Zhang
Xuwei Wu
Yi Xiao
Mei Chen
Zhidong Li
Xing Wei
Kaifa Tang
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2014
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-014-0067-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancer

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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