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World Journal of Surgery

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Open Access 01-05-2017 | Original Scientific Report

Genetic and Epigenetic Intra-tumour Heterogeneity in Colorectal Cancer

Authors: Huw Geraint Jones, Gareth Jenkins, Namor Williams, Paul Griffiths, Phil Chambers, John Beynon, Dean Harris

Published in: World Journal of Surgery | Issue 5/2017

Abstract

Introduction

Colorectal cancer (CRC) is a highly heterogeneous disease, with pathologically similar cancers having completely different responses to treatment and patient survival. Intra-tumour heterogeneity (defined as distinct morphological and phenotypic differences) has recently been demonstrated to be an important factor in the development and behaviour of cancer cells and can be used to determine response to anticancer therapy.

Method

Patients with resected CRC had DNA extracted from eight defined tumour areas which were analysed for two genetic mutations (BRAF and KRAS) and one epigenetic trait (CpG island methylator phenotype/CIMP). Normal adjacent tissue was studied as control.

Results

Twelve patients with CRC were included. Intra-tumoural heterogeneity for KRAS mutation was seen in 2 patients (17%). There was no statistical evidence of CIMP status heterogeneity (p = 0.85), but 6 of the 12 patients (50%) demonstrated at least one heterogeneous area within the tumour.

Discussion

Intra-tumoural heterogeneity for both genetic and epigenetic factors in CRC is more prevalent than previously thought in Stage II and Stage III CRC. This study provides new insight into epigenetic heterogeneity of CRC and supports the development of a more targeted biopsy strategy to support expansion of personalised treatment.

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Title: Genetic and Epigenetic Intra-tumour Heterogeneity in Colorectal Cancer
Authors: Huw Geraint Jones
Gareth Jenkins
Namor Williams
Paul Griffiths
Phil Chambers
John Beynon
Dean Harris
Publication date: 01-05-2017
Publisher: Springer International Publishing
Published in: World Journal of Surgery / Issue 5/2017
Print ISSN: 0364-2313
Electronic ISSN: 1432-2323
DOI: https://doi.org/10.1007/s00268-016-3860-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Genetic and Epigenetic Intra-tumour Heterogeneity in Colorectal Cancer

Abstract

Introduction

Method

Results

Discussion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Method

Results

Discussion

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