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Archives of Virology
Published in: Archives of Virology 8/2014

01-08-2014 | Brief Report

Generic detection and identification of pospiviroids

Authors: Thibaut Olivier, Elisabeth Demonty, Frédéric Fauche, Stéphan Steyer

Published in: Archives of Virology | Issue 8/2014

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Abstract

A multiplex one-step RT-PCR aiming at detecting all pospiviroids known to be harmful to cultivated plants has been developed. Specificity, sensitivity, selectivity, repeatability and reproducibility of this test have been assessed in order to fulfill the recommendations of the EPPO standard PM7/98 and provide routine detection laboratories with a cost-effective, easy-to-use and robust pospiviroid detection test. To further understand the epidemiology and ease the management of pospiviroid outbreaks, this RT-PCR diagnostic test can be followed by direct sequencing of the amplicons to identify and characterize the detected pospiviroid isolates.
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Metadata
Title
Generic detection and identification of pospiviroids
Authors
Thibaut Olivier
Elisabeth Demonty
Frédéric Fauche
Stéphan Steyer
Publication date
01-08-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-1978-6

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