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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2021 | Gene Therapy in Oncology | Research

Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors

Authors: Lili Ye, Yaoming Chen, Jizong Mao, Xiaotian Lei, Qian Yang, Chunhui Cui

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2021

Abstract

Background

The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.

Methods

We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer.

Results

The results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection.

Conclusion

Our findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor.

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Title: Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors
Authors: Lili Ye
Yaoming Chen
Jizong Mao
Xiaotian Lei
Qian Yang
Chunhui Cui
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Gene Therapy in Oncology
Laser
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2021
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/s13046-021-02105-3

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Abstract

Background

Methods

Results

Conclusion

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