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01-08-2019 | Gene Therapy in Oncology | Original Article

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

Authors: Hori Ghaneialvar, Abbas Sahebghadam Lotfi, Sareh Arjmand, Leila Soltani, Azra Kenarkoohi, Mohammad Mahdi Zangeneh

Published in: Comparative Clinical Pathology | Issue 4/2019

Abstract

Due to their unique biological characteristics, mesenchymal stem cells (MSCs) are useful in developing cell-based therapies, tissue regeneration, and many other applications of cellular and molecular genetics. Use of lentiviral system allows the integration of specific nucleic acid sequence into a target cell genome near an active transcription site in such a way that it is stable and heritable. The aim of the present study was to evaluate the gene transduction efficiency on the cells of different species using lentivirus along with cytomegalovirus in a lentiviral shuttle vector. In this study, the third-passage isolated MSCs from four different species, including ovine and goat cardiac fibroblasts, HEK293T, and L929 cell line were transduced by concentrated virus particles. Then, transduction percentage was detected by flow cytometry. The findings showed different transduction efficiencies for various cells of one species or other species, indicating that differentiated and undifferentiated cells had different transduction percentages. Different degrees of transduction observed in differentiated and undifferentiated cells might be the result of various lentiviral receptors on the plasma membrane, site of integration, and activity of CMV promoter. Further, other regulatory factors involved in cell transduction and target gene expression can cause different percentages of transduction.

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Title: Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter
Authors: Hori Ghaneialvar
Abbas Sahebghadam Lotfi
Sareh Arjmand
Leila Soltani
Azra Kenarkoohi
Mohammad Mahdi Zangeneh
Publication date: 01-08-2019
Publisher: Springer London
Keyword: Gene Therapy in Oncology
Published in: Comparative Clinical Pathology / Issue 4/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02929-8

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Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

Abstract

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