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01-12-2009 | Original Article

Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

Authors: Christian Morsczeck, Gottfried Schmalz, Torsten Eugen Reichert, Florian Völlner, Michael Saugspier, Sandra Viale-Bouroncle, Oliver Driemel

Published in: Clinical Oral Investigations | Issue 4/2009

Abstract

Recently, osteogenic precursor cells were isolated from human dental follicles, which differentiate into cementoblast- or osteoblast-like cells under in vitro conditions after the induction with dexamethasone or insulin. However, mechanisms for osteogenic differentiation are not understood in detail. In a previous study, real-time RT-PCR results demonstrated molecular mechanisms in dental follicle cells (DFCs) during osteogenic differentiation that are different from those in bone-marrow-derived mesenchymal stem cells. We analysed gene expression profiles in DFCs before and after osteogenic differentiation with the Affymetrix GeneChip® Human Gene 1.0 ST Array. Transcripts of 98 genes were up-regulated after differentiation. These genes could be clustered into subcategories such as cell differentiation, cell morphogenesis, and skeletal development. Osteoblast-specific transcription factors like osterix and runx2 were constitutively expressed in differentiated DFCs. In contrast, the transcription factor ZBTB16, which promotes the osteoblastic differentiation of mesenchymal stem cells as an up-stream regulator of runx2, was differentially expressed after differentiation. Transcription factors NR4A3, KLF9 and TSC22D3, involved in the regulation of cellular development, were up-regulated as well. In conclusion, we present the first transcriptome of human DFCs before and after osteogenic differentiation. This study sheds new light on the complex mechanism of osteogenic differentiation in DFCs.

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Title: Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro
Authors: Christian Morsczeck
Gottfried Schmalz
Torsten Eugen Reichert
Florian Völlner
Michael Saugspier
Sandra Viale-Bouroncle
Oliver Driemel
Publication date: 01-12-2009
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 4/2009
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-009-0260-x

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Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

Abstract

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