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Published in: Molecular Cancer 1/2008

Open Access 01-12-2008 | Research

Gene expression profiles in primary pancreatic tumors and metastatic lesions of Ela-c-myc transgenic mice

Authors: Archana Thakur, Aliccia Bollig, Jiusheng Wu, Dezhong J Liao

Published in: Molecular Cancer | Issue 1/2008

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Abstract

Background

Pancreatic carcinoma usually is a fatal disease with no cure, mainly due to its invasion and metastasis prior to diagnosis. We analyzed the gene expression profiles of paired primary pancreatic tumors and metastatic lesions from Ela-c-myc transgenic mice in order to identify genes that may be involved in the pancreatic cancer progression. Differentially expressed selected genes were verified by semi-quantitative and quantitative RT-PCR. To further evaluate the relevance of some of the selected differentially expressed genes, we investigated their expression pattern in human pancreatic cancer cell lines with high and low metastatic potentials.

Results

Data indicate that genes involved in posttranscriptional regulation were a major functional category of upregulated genes in both primary pancreatic tumors (PT) and liver metastatic lesions (LM) compared to normal pancreas (NP). In particular, differential expression for splicing factors, RNA binding/pre-mRNA processing factors and spliceosome related genes were observed, indicating that RNA processing and editing related events may play critical roles in pancreatic tumor development and progression. High expression of insulin growth factor binding protein-1 (Igfbp1) and Serine proteinase inhibitor A1 (Serpina1), and low levels or absence of Wt1 gene expression were exclusive to liver metastatic lesion samples.

Conclusion

We identified Igfbp1, Serpina1 and Wt1 genes that are likely to be clinically useful biomarkers for prognostic or therapeutic purposes in metastatic pancreatic cancer, particularly in pancreatic cancer where c-Myc is overexpressed.
Appendix
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Literature
1.
go back to reference Yeo TP, Hruban RH, Leach SD, Wilentz RE, Sohn TA, Kern SE, Iacobuzio-Donahue CA, Maitra A, Goggins M, Canto MI: Pancreatic cancer. Curr Probl Cancer. 2002, 26 (4): 176-275.CrossRefPubMed Yeo TP, Hruban RH, Leach SD, Wilentz RE, Sohn TA, Kern SE, Iacobuzio-Donahue CA, Maitra A, Goggins M, Canto MI: Pancreatic cancer. Curr Probl Cancer. 2002, 26 (4): 176-275.CrossRefPubMed
2.
go back to reference Schleger C, Verbeke C, Hildenbrand R, Zentgraf H, Bleyl U: c-MYC activation in primary and metastatic ductal adenocarcinoma of the pancreas: incidence, mechanisms, and clinical significance. Mod Pathol. 2002, 15 (4): 462-469.CrossRefPubMed Schleger C, Verbeke C, Hildenbrand R, Zentgraf H, Bleyl U: c-MYC activation in primary and metastatic ductal adenocarcinoma of the pancreas: incidence, mechanisms, and clinical significance. Mod Pathol. 2002, 15 (4): 462-469.CrossRefPubMed
3.
go back to reference Mahlamaki EH, Barlund M, Tanner M, Gorunova L, Hoglund M, Karhu R, Kallioniemi A: Frequent amplification of 8q24, 11q, 17q, and 20q-specific genes in pancreatic cancer. Genes Chromosomes Cancer. 2002, 35 (4): 353-358.CrossRefPubMed Mahlamaki EH, Barlund M, Tanner M, Gorunova L, Hoglund M, Karhu R, Kallioniemi A: Frequent amplification of 8q24, 11q, 17q, and 20q-specific genes in pancreatic cancer. Genes Chromosomes Cancer. 2002, 35 (4): 353-358.CrossRefPubMed
4.
go back to reference Yamada H, Sakamoto H, Taira M, Nishimura S, Shimosato Y, Terada M, Sugimura T: Amplifications of both c-Ki-ras with a point mutation and c-myc in a primary pancreatic cancer and its metastatic tumors in lymph nodes. Jpn J Cancer Res. 1986, 77 (4): 370-375.PubMed Yamada H, Sakamoto H, Taira M, Nishimura S, Shimosato Y, Terada M, Sugimura T: Amplifications of both c-Ki-ras with a point mutation and c-myc in a primary pancreatic cancer and its metastatic tumors in lymph nodes. Jpn J Cancer Res. 1986, 77 (4): 370-375.PubMed
5.
go back to reference Sandgren EP, Quaife CJ, Paulovich AG, Palmiter RD, Brinster RL: Pancreatic tumor pathogenesis reflects the causative genetic lesion. Proc Natl Acad Sci USA. 1991, 88 (1): 93-97.PubMedCentralCrossRefPubMed Sandgren EP, Quaife CJ, Paulovich AG, Palmiter RD, Brinster RL: Pancreatic tumor pathogenesis reflects the causative genetic lesion. Proc Natl Acad Sci USA. 1991, 88 (1): 93-97.PubMedCentralCrossRefPubMed
6.
go back to reference Liao DJ, Wang Y, Wu J, Adsay NV, Grignon D, Khanani F, Sarkar FH: Characterization of pancreatic lesions from MT-tgfalpha, Ela-myc and MT-tgfalpha/Ela-myc single and double transgenic mice. J Carcinog. 2006, 5: 19-PubMedCentralCrossRefPubMed Liao DJ, Wang Y, Wu J, Adsay NV, Grignon D, Khanani F, Sarkar FH: Characterization of pancreatic lesions from MT-tgfalpha, Ela-myc and MT-tgfalpha/Ela-myc single and double transgenic mice. J Carcinog. 2006, 5: 19-PubMedCentralCrossRefPubMed
7.
go back to reference Liao JD, Adsay NV, Khannani F, Grignon D, Thakur A, Sarkar FH: Histological complexities of pancreatic lesions from transgenic mouse models are consistent with biological and morphological heterogeneity of human pancreatic cancer. Histol Histopathol. 2007, 22 (6): 661-676.PubMedCentralPubMed Liao JD, Adsay NV, Khannani F, Grignon D, Thakur A, Sarkar FH: Histological complexities of pancreatic lesions from transgenic mouse models are consistent with biological and morphological heterogeneity of human pancreatic cancer. Histol Histopathol. 2007, 22 (6): 661-676.PubMedCentralPubMed
9.
go back to reference Kleeff J, Friess H, Berberat PO, Martignoni ME, Z'Graggen K, Buchler MW: Pancreatic cancer – new aspects of molecular biology research. Swiss Surg. 2000, 6 (5): 231-234.CrossRefPubMed Kleeff J, Friess H, Berberat PO, Martignoni ME, Z'Graggen K, Buchler MW: Pancreatic cancer – new aspects of molecular biology research. Swiss Surg. 2000, 6 (5): 231-234.CrossRefPubMed
10.
go back to reference Iacobuzio-Donahue CA, Maitra A, Olsen M, Lowe AW, van Heek NT, Rosty C, Walter K, Sato N, Parker A, Ashfaq R: Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol. 2003, 162 (4): 1151-1162.PubMedCentralCrossRefPubMed Iacobuzio-Donahue CA, Maitra A, Olsen M, Lowe AW, van Heek NT, Rosty C, Walter K, Sato N, Parker A, Ashfaq R: Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol. 2003, 162 (4): 1151-1162.PubMedCentralCrossRefPubMed
11.
go back to reference Iacobuzio-Donahue CA, Maitra A, Shen-Ong GL, van Heek T, Ashfaq R, Meyer R, Walter K, Berg K, Hollingsworth MA, Cameron JL: Discovery of novel tumor markers of pancreatic cancer using global gene expression technology. Am J Pathol. 2002, 160 (4): 1239-1249.PubMedCentralCrossRefPubMed Iacobuzio-Donahue CA, Maitra A, Shen-Ong GL, van Heek T, Ashfaq R, Meyer R, Walter K, Berg K, Hollingsworth MA, Cameron JL: Discovery of novel tumor markers of pancreatic cancer using global gene expression technology. Am J Pathol. 2002, 160 (4): 1239-1249.PubMedCentralCrossRefPubMed
12.
go back to reference Maacke H, Jost K, Opitz S, Miska S, Yuan Y, Hasselbach L, Luttges J, Kalthoff H, Sturzbecher HW: DNA repair and recombination factor Rad51 is over-expressed in human pancreatic adenocarcinoma. Oncogene. 2000, 19 (23): 2791-2795.CrossRefPubMed Maacke H, Jost K, Opitz S, Miska S, Yuan Y, Hasselbach L, Luttges J, Kalthoff H, Sturzbecher HW: DNA repair and recombination factor Rad51 is over-expressed in human pancreatic adenocarcinoma. Oncogene. 2000, 19 (23): 2791-2795.CrossRefPubMed
13.
go back to reference Lynch HT, Deters CA, Snyder CL, Lynch JF, Villeneuve P, Silberstein J, Martin H, Narod SA, Brand RE: BRCA1 and pancreatic cancer: pedigree findings and their causal relationships. Cancer Genet Cytogenet. 2005, 158 (2): 119-125.CrossRefPubMed Lynch HT, Deters CA, Snyder CL, Lynch JF, Villeneuve P, Silberstein J, Martin H, Narod SA, Brand RE: BRCA1 and pancreatic cancer: pedigree findings and their causal relationships. Cancer Genet Cytogenet. 2005, 158 (2): 119-125.CrossRefPubMed
14.
go back to reference Mahlamaki EH, Kauraniemi P, Monni O, Wolf M, Hautaniemi S, Kallioniemi A: High-resolution genomic and expression profiling reveals 105 putative amplification target genes in pancreatic cancer. Neoplasia. 2004, 6 (5): 432-439.PubMedCentralCrossRefPubMed Mahlamaki EH, Kauraniemi P, Monni O, Wolf M, Hautaniemi S, Kallioniemi A: High-resolution genomic and expression profiling reveals 105 putative amplification target genes in pancreatic cancer. Neoplasia. 2004, 6 (5): 432-439.PubMedCentralCrossRefPubMed
15.
go back to reference Nakamura T, Fidler IJ, Coombes KR: Gene expression profile of metastatic human pancreatic cancer cells depends on the organ microenvironment. Cancer Res. 2007, 67 (1): 139-148.CrossRefPubMed Nakamura T, Fidler IJ, Coombes KR: Gene expression profile of metastatic human pancreatic cancer cells depends on the organ microenvironment. Cancer Res. 2007, 67 (1): 139-148.CrossRefPubMed
16.
go back to reference Nakamura T, Furukawa Y, Nakagawa H, Tsunoda T, Ohigashi H, Murata K, Ishikawa O, Ohgaki K, Kashimura N, Miyamoto M: Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissection. Oncogene. 2004, 23 (13): 2385-2400.CrossRefPubMed Nakamura T, Furukawa Y, Nakagawa H, Tsunoda T, Ohigashi H, Murata K, Ishikawa O, Ohgaki K, Kashimura N, Miyamoto M: Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissection. Oncogene. 2004, 23 (13): 2385-2400.CrossRefPubMed
17.
go back to reference Soling A, Sackewitz M, Volkmar M, Schaarschmidt D, Jacob R, Holzhausen HJ, Rainov NG: Minichromosome maintenance protein 3 elicits a cancer-restricted immune response in patients with brain malignancies and is a strong independent predictor of survival in patients with anaplastic astrocytoma. Clin Cancer Res. 2005, 11 (1): 249-258.PubMed Soling A, Sackewitz M, Volkmar M, Schaarschmidt D, Jacob R, Holzhausen HJ, Rainov NG: Minichromosome maintenance protein 3 elicits a cancer-restricted immune response in patients with brain malignancies and is a strong independent predictor of survival in patients with anaplastic astrocytoma. Clin Cancer Res. 2005, 11 (1): 249-258.PubMed
18.
go back to reference Sanada Y, Oue N, Mitani Y, Yoshida K, Nakayama H, Yasui W: Down-regulation of the claudin-18 gene, identified through serial analysis of gene expression data analysis, in gastric cancer with an intestinal phenotype. J Pathol. 2006, 208 (5): 633-642.CrossRefPubMed Sanada Y, Oue N, Mitani Y, Yoshida K, Nakayama H, Yasui W: Down-regulation of the claudin-18 gene, identified through serial analysis of gene expression data analysis, in gastric cancer with an intestinal phenotype. J Pathol. 2006, 208 (5): 633-642.CrossRefPubMed
19.
go back to reference Yonezawa S, Byrd JC, Dahiya R, Ho JJ, Gum JR, Griffiths B, Swallow DM, Kim YS: Differential mucin gene expression in human pancreatic and colon cancer cells. Biochem J. 1991, 276 (Pt 3): 599-605.PubMedCentralCrossRefPubMed Yonezawa S, Byrd JC, Dahiya R, Ho JJ, Gum JR, Griffiths B, Swallow DM, Kim YS: Differential mucin gene expression in human pancreatic and colon cancer cells. Biochem J. 1991, 276 (Pt 3): 599-605.PubMedCentralCrossRefPubMed
20.
go back to reference Hollingsworth MA, Swanson BJ: Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer. 2004, 4 (1): 45-60.CrossRefPubMed Hollingsworth MA, Swanson BJ: Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer. 2004, 4 (1): 45-60.CrossRefPubMed
21.
go back to reference Resar LM, Dolde C, Barrett JF, Dang CV: B-myc inhibits neoplastic transformation and transcriptional activation by c-myc. Mol Cell Biol. 1993, 13 (2): 1130-1136.PubMedCentralPubMed Resar LM, Dolde C, Barrett JF, Dang CV: B-myc inhibits neoplastic transformation and transcriptional activation by c-myc. Mol Cell Biol. 1993, 13 (2): 1130-1136.PubMedCentralPubMed
22.
go back to reference Griffith OL, Melck A, Jones SJ, Wiseman SM: Meta-analysis and meta-review of thyroid cancer gene expression profiling studies identifies important diagnostic biomarkers. J Clin Oncol. 2006, 24 (31): 5043-5051.CrossRefPubMed Griffith OL, Melck A, Jones SJ, Wiseman SM: Meta-analysis and meta-review of thyroid cancer gene expression profiling studies identifies important diagnostic biomarkers. J Clin Oncol. 2006, 24 (31): 5043-5051.CrossRefPubMed
23.
go back to reference Moore LE, Fung ET, McGuire M, Rabkin CC, Molinaro A, Wang Z, Zhang F, Wang J, Yip C, Meng XY: Evaluation of apolipoprotein A1 and posttranslationally modified forms of transthyretin as biomarkers for ovarian cancer detection in an independent study population. Cancer Epidemiol Biomarkers Prev. 2006, 15 (9): 1641-1646.CrossRefPubMed Moore LE, Fung ET, McGuire M, Rabkin CC, Molinaro A, Wang Z, Zhang F, Wang J, Yip C, Meng XY: Evaluation of apolipoprotein A1 and posttranslationally modified forms of transthyretin as biomarkers for ovarian cancer detection in an independent study population. Cancer Epidemiol Biomarkers Prev. 2006, 15 (9): 1641-1646.CrossRefPubMed
24.
go back to reference Weinstein PS, Skinner M, Sipe JD, Lokich JJ, Zamcheck N, Cohen AS: Acute-phase proteins or tumour markers: the role of SAA, SAP, CRP and CEA as indicators of metastasis in a broad spectrum of neoplastic diseases. Scand J Immunol. 1984, 19 (3): 193-198.CrossRefPubMed Weinstein PS, Skinner M, Sipe JD, Lokich JJ, Zamcheck N, Cohen AS: Acute-phase proteins or tumour markers: the role of SAA, SAP, CRP and CEA as indicators of metastasis in a broad spectrum of neoplastic diseases. Scand J Immunol. 1984, 19 (3): 193-198.CrossRefPubMed
25.
go back to reference Sato N, Fukushima N, Maitra A, Iacobuzio-Donahue CA, van Heek NT, Cameron JL, Yeo CJ, Hruban RH, Goggins M: Gene expression profiling identifies genes associated with invasive intraductal papillary mucinous neoplasms of the pancreas. Am J Pathol. 2004, 164 (3): 903-914.PubMedCentralCrossRefPubMed Sato N, Fukushima N, Maitra A, Iacobuzio-Donahue CA, van Heek NT, Cameron JL, Yeo CJ, Hruban RH, Goggins M: Gene expression profiling identifies genes associated with invasive intraductal papillary mucinous neoplasms of the pancreas. Am J Pathol. 2004, 164 (3): 903-914.PubMedCentralCrossRefPubMed
26.
go back to reference Lian Z, De Luca P, Di Cristofano A: Gene expression analysis reveals a signature of estrogen receptor activation upon loss of Pten in a mouse model of endometrial cancer. J Cell Physiol. 2006, 208 (2): 255-266.CrossRefPubMed Lian Z, De Luca P, Di Cristofano A: Gene expression analysis reveals a signature of estrogen receptor activation upon loss of Pten in a mouse model of endometrial cancer. J Cell Physiol. 2006, 208 (2): 255-266.CrossRefPubMed
27.
go back to reference Liu AY, Zhang H, Sorensen CM, Diamond DL: Analysis of prostate cancer by proteomics using tissue specimens. J Urol. 2005, 173 (1): 73-78.CrossRefPubMed Liu AY, Zhang H, Sorensen CM, Diamond DL: Analysis of prostate cancer by proteomics using tissue specimens. J Urol. 2005, 173 (1): 73-78.CrossRefPubMed
28.
go back to reference Mauri P, Scarpa A, Nascimbeni AC, Benazzi L, Parmagnani E, Mafficini A, Della Peruta M, Bassi C, Miyazaki K, Sorio C: Identification of proteins released by pancreatic cancer cells by multidimensional protein identification technology: a strategy for identification of novel cancer markers. Faseb J. 2005, 19 (9): 1125-1127.PubMed Mauri P, Scarpa A, Nascimbeni AC, Benazzi L, Parmagnani E, Mafficini A, Della Peruta M, Bassi C, Miyazaki K, Sorio C: Identification of proteins released by pancreatic cancer cells by multidimensional protein identification technology: a strategy for identification of novel cancer markers. Faseb J. 2005, 19 (9): 1125-1127.PubMed
29.
go back to reference Hansel DE, Rahman A, House M, Ashfaq R, Berg K, Yeo CJ, Maitra A: Met proto-oncogene and insulin-like growth factor binding protein 3 overexpression correlates with metastatic ability in well-differentiated pancreatic endocrine neoplasms. Clin Cancer Res. 2004, 10 (18 Pt 1): 6152-6158.CrossRefPubMed Hansel DE, Rahman A, House M, Ashfaq R, Berg K, Yeo CJ, Maitra A: Met proto-oncogene and insulin-like growth factor binding protein 3 overexpression correlates with metastatic ability in well-differentiated pancreatic endocrine neoplasms. Clin Cancer Res. 2004, 10 (18 Pt 1): 6152-6158.CrossRefPubMed
30.
go back to reference Karna E, Surazynski A, Orlowski K, Laszkiewicz J, Puchalski Z, Nawrat P, Palka J: Serum and tissue level of insulin-like growth factor-I (IGF-I) and IGF-I binding proteins as an index of pancreatitis and pancreatic cancer. Int J Exp Pathol. 2002, 83 (5): 239-245.PubMedCentralCrossRefPubMed Karna E, Surazynski A, Orlowski K, Laszkiewicz J, Puchalski Z, Nawrat P, Palka J: Serum and tissue level of insulin-like growth factor-I (IGF-I) and IGF-I binding proteins as an index of pancreatitis and pancreatic cancer. Int J Exp Pathol. 2002, 83 (5): 239-245.PubMedCentralCrossRefPubMed
32.
go back to reference Yamada S, Ohira M, Horie H, Ando K, Takayasu H, Suzuki Y, Sugano S, Hirata T, Goto T, Matsunaga T: Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas. Oncogene. 2004, 23 (35): 5901-5911.CrossRefPubMed Yamada S, Ohira M, Horie H, Ando K, Takayasu H, Suzuki Y, Sugano S, Hirata T, Goto T, Matsunaga T: Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas. Oncogene. 2004, 23 (35): 5901-5911.CrossRefPubMed
33.
go back to reference Johnson JM, Castle J, Garrett-Engele P, Kan Z, Loerch PM, Armour CD, Santos R, Schadt EE, Stoughton R, Shoemaker DD: Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science. 2003, 302 (5653): 2141-2144.CrossRefPubMed Johnson JM, Castle J, Garrett-Engele P, Kan Z, Loerch PM, Armour CD, Santos R, Schadt EE, Stoughton R, Shoemaker DD: Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science. 2003, 302 (5653): 2141-2144.CrossRefPubMed
34.
go back to reference Pajares MJ, Ezponda T, Catena R, Calvo A, Pio R, Montuenga LM: Alternative splicing: an emerging topic in molecular and clinical oncology. Lancet Oncol. 2007, 8 (4): 349-357.CrossRefPubMed Pajares MJ, Ezponda T, Catena R, Calvo A, Pio R, Montuenga LM: Alternative splicing: an emerging topic in molecular and clinical oncology. Lancet Oncol. 2007, 8 (4): 349-357.CrossRefPubMed
35.
go back to reference Srebrow A, Kornblihtt AR: The connection between splicing and cancer. J Cell Sci. 2006, 119 (Pt 13): 2635-2641.CrossRefPubMed Srebrow A, Kornblihtt AR: The connection between splicing and cancer. J Cell Sci. 2006, 119 (Pt 13): 2635-2641.CrossRefPubMed
36.
37.
38.
go back to reference Hayes GM, Carrigan PE, Beck AM, Miller LJ: Targeting the RNA splicing machinery as a novel treatment strategy for pancreatic carcinoma. Cancer Res. 2006, 66 (7): 3819-3827.CrossRefPubMed Hayes GM, Carrigan PE, Beck AM, Miller LJ: Targeting the RNA splicing machinery as a novel treatment strategy for pancreatic carcinoma. Cancer Res. 2006, 66 (7): 3819-3827.CrossRefPubMed
39.
go back to reference Zahler AM, Neugebauer KM, Lane WS, Roth MB: Distinct functions of SR proteins in alternative pre-mRNA splicing. Science. 1993, 260 (5105): 219-222.CrossRefPubMed Zahler AM, Neugebauer KM, Lane WS, Roth MB: Distinct functions of SR proteins in alternative pre-mRNA splicing. Science. 1993, 260 (5105): 219-222.CrossRefPubMed
40.
go back to reference Stickeler E, Kittrell F, Medina D, Berget SM: Stage-specific changes in SR splicing factors and alternative splicing in mammary tumorigenesis. Oncogene. 1999, 18 (24): 3574-3582.CrossRefPubMed Stickeler E, Kittrell F, Medina D, Berget SM: Stage-specific changes in SR splicing factors and alternative splicing in mammary tumorigenesis. Oncogene. 1999, 18 (24): 3574-3582.CrossRefPubMed
41.
go back to reference Bailis JM, Forsburg SL: MCM proteins: DNA damage, mutagenesis and repair. Curr Opin Genet Dev. 2004, 14 (1): 17-21.CrossRefPubMed Bailis JM, Forsburg SL: MCM proteins: DNA damage, mutagenesis and repair. Curr Opin Genet Dev. 2004, 14 (1): 17-21.CrossRefPubMed
42.
go back to reference Hirano T: At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol. 2006, 7 (5): 311-322.CrossRefPubMed Hirano T: At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol. 2006, 7 (5): 311-322.CrossRefPubMed
43.
go back to reference Amor DJ, Kalitsis P, Sumer H, Choo KH: Building the centromere: from foundation proteins to 3D organization. Trends Cell Biol. 2004, 14 (7): 359-368.CrossRefPubMed Amor DJ, Kalitsis P, Sumer H, Choo KH: Building the centromere: from foundation proteins to 3D organization. Trends Cell Biol. 2004, 14 (7): 359-368.CrossRefPubMed
44.
go back to reference Khanna KK, Jackson SP: DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet. 2001, 27 (3): 247-254.CrossRefPubMed Khanna KK, Jackson SP: DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet. 2001, 27 (3): 247-254.CrossRefPubMed
45.
go back to reference Kataoka H, Itoh H, Koono M: Emerging multifunctional aspects of cellular serine proteinase inhibitors in tumor progression and tissue regeneration. Pathol Int. 2002, 52 (2): 89-102.CrossRefPubMed Kataoka H, Itoh H, Koono M: Emerging multifunctional aspects of cellular serine proteinase inhibitors in tumor progression and tissue regeneration. Pathol Int. 2002, 52 (2): 89-102.CrossRefPubMed
46.
go back to reference Firth SM, Baxter RC: Cellular actions of the insulin-like growth factor binding proteins. Endocr Rev. 2002, 23 (6): 824-854.CrossRefPubMed Firth SM, Baxter RC: Cellular actions of the insulin-like growth factor binding proteins. Endocr Rev. 2002, 23 (6): 824-854.CrossRefPubMed
47.
go back to reference Perks CM, Newcomb PV, Norman MR, Holly JM: Effect of insulin-like growth factor binding protein-1 on integrin signalling and the induction of apoptosis in human breast cancer cells. J Mol Endocrinol. 1999, 22 (2): 141-150.CrossRefPubMed Perks CM, Newcomb PV, Norman MR, Holly JM: Effect of insulin-like growth factor binding protein-1 on integrin signalling and the induction of apoptosis in human breast cancer cells. J Mol Endocrinol. 1999, 22 (2): 141-150.CrossRefPubMed
48.
go back to reference Jones JI, Doerr ME, Clemmons DR: Cell migration: interactions among integrins, IGFs and IGFBPs. Prog Growth Factor Res. 1995, 6 (2–4): 319-327.CrossRefPubMed Jones JI, Doerr ME, Clemmons DR: Cell migration: interactions among integrins, IGFs and IGFBPs. Prog Growth Factor Res. 1995, 6 (2–4): 319-327.CrossRefPubMed
49.
go back to reference Petrache I, Fijalkowska I, Zhen L, Medler TR, Brown E, Cruz P, Choe KH, Taraseviciene-Stewart L, Scerbavicius R, Shapiro L: A novel antiapoptotic role for alpha1-antitrypsin in the prevention of pulmonary emphysema. Am J Respir Crit Care Med. 2006, 173 (11): 1222-1228.PubMedCentralCrossRefPubMed Petrache I, Fijalkowska I, Zhen L, Medler TR, Brown E, Cruz P, Choe KH, Taraseviciene-Stewart L, Scerbavicius R, Shapiro L: A novel antiapoptotic role for alpha1-antitrypsin in the prevention of pulmonary emphysema. Am J Respir Crit Care Med. 2006, 173 (11): 1222-1228.PubMedCentralCrossRefPubMed
50.
go back to reference Zelvyte I, Wallmark A, Piitulainen E, Westin U, Janciauskiene S: Increased plasma levels of serine proteinase inhibitors in lung cancer patients. Anticancer Res. 2004, 24 (1): 241-247.PubMed Zelvyte I, Wallmark A, Piitulainen E, Westin U, Janciauskiene S: Increased plasma levels of serine proteinase inhibitors in lung cancer patients. Anticancer Res. 2004, 24 (1): 241-247.PubMed
51.
go back to reference Trichopoulos D, Tzonou A, Kalapothaki V, Sparos L, Kremastinou T, Skoutari M: Alpha 1-antitrypsin and survival in pancreatic cancer. Int J Cancer. 1990, 45 (4): 685-686.CrossRefPubMed Trichopoulos D, Tzonou A, Kalapothaki V, Sparos L, Kremastinou T, Skoutari M: Alpha 1-antitrypsin and survival in pancreatic cancer. Int J Cancer. 1990, 45 (4): 685-686.CrossRefPubMed
52.
go back to reference Tzonou A, Sparos L, Kalapothaki V, Zavitsanos X, Rebelakos A, Trichopoulos D: Alpha 1-antitrypsin and survival in hepatocellular carcinoma. Br J Cancer. 1990, 61 (1): 72-73.PubMedCentralCrossRefPubMed Tzonou A, Sparos L, Kalapothaki V, Zavitsanos X, Rebelakos A, Trichopoulos D: Alpha 1-antitrypsin and survival in hepatocellular carcinoma. Br J Cancer. 1990, 61 (1): 72-73.PubMedCentralCrossRefPubMed
53.
go back to reference Higashiyama M, Doi O, Kodama K, Yokouchi H, Tateishi R: An evaluation of the prognostic significance of alpha-1-antitrypsin expression in adenocarcinomas of the lung: an immunohistochemical analysis. Br J Cancer. 1992, 65 (2): 300-302.PubMedCentralCrossRefPubMed Higashiyama M, Doi O, Kodama K, Yokouchi H, Tateishi R: An evaluation of the prognostic significance of alpha-1-antitrypsin expression in adenocarcinomas of the lung: an immunohistochemical analysis. Br J Cancer. 1992, 65 (2): 300-302.PubMedCentralCrossRefPubMed
54.
go back to reference Sun Z, Yang P: Role of imbalance between neutrophil elastase and alpha 1-antitrypsin in cancer development and progression. Lancet Oncol. 2004, 5 (3): 182-190.CrossRefPubMed Sun Z, Yang P: Role of imbalance between neutrophil elastase and alpha 1-antitrypsin in cancer development and progression. Lancet Oncol. 2004, 5 (3): 182-190.CrossRefPubMed
55.
go back to reference Higashiyama M, Doi O, Kodama K, Yokouchi H, Tateishi R, Matsuura N, Murata A, Tomita N, Monden T, Ogawa M: Immunohistochemical analysis of pancreatic secretory trypsin inhibitor expression in pulmonary adenocarcinoma: its possible participation in scar formation of the tumor tissues. Tumour Biol. 1992, 13 (5–6): 299-307.CrossRefPubMed Higashiyama M, Doi O, Kodama K, Yokouchi H, Tateishi R, Matsuura N, Murata A, Tomita N, Monden T, Ogawa M: Immunohistochemical analysis of pancreatic secretory trypsin inhibitor expression in pulmonary adenocarcinoma: its possible participation in scar formation of the tumor tissues. Tumour Biol. 1992, 13 (5–6): 299-307.CrossRefPubMed
56.
go back to reference Thakur A, Xu H, Wang Y, Bollig A, Biliran H, Liao JD: The role of X-linked genes in breast cancer. Breast Cancer Res Treat. 2005, 93 (2): 135-143.CrossRefPubMed Thakur A, Xu H, Wang Y, Bollig A, Biliran H, Liao JD: The role of X-linked genes in breast cancer. Breast Cancer Res Treat. 2005, 93 (2): 135-143.CrossRefPubMed
57.
go back to reference Oji Y, Nakamori S, Fujikawa M, Nakatsuka S, Yokota A, Tatsumi N, Abeno S, Ikeba A, Takashima S, Tsujie M: Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma. Cancer Sci. 2004, 95 (7): 583-7.CrossRefPubMed Oji Y, Nakamori S, Fujikawa M, Nakatsuka S, Yokota A, Tatsumi N, Abeno S, Ikeba A, Takashima S, Tsujie M: Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma. Cancer Sci. 2004, 95 (7): 583-7.CrossRefPubMed
Metadata
Title
Gene expression profiles in primary pancreatic tumors and metastatic lesions of Ela-c-myc transgenic mice
Authors
Archana Thakur
Aliccia Bollig
Jiusheng Wu
Dezhong J Liao
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2008
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/1476-4598-7-11

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Molecular Cancer 1/2008 Go to the issue
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Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
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