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Cancer Cell International
Published in: Cancer Cell International 1/2020

01-12-2020 | Gastric Cancer | Primary research

CircPIP5K1A facilitates gastric cancer progression via miR-376c-3p/ZNF146 axis

Authors: Yan Ma, Xiliang Cong, Yiyun Zhang, Xin Yin, Ziyu Zhu, Yingwei Xue

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Recently, many emerging circular RNAs (circRNAs) have been studied in human malignancies, including gastric cancer (GC). Researches concerning cancers have revealed that aberrant expression of circRNAs play a big part in tumorigenesis and development of diverse malignant tumors. Although hsa_circ_0014130 (circPIP5K1A) has been confirmed to be closely related to non-small cell lung cancer (NSCLC) progression, the knowledge of its function on GC progression remains unclear. Therefore, it is of great interest to uncover the underlying role of circPIP5K1A in GC.

Methods

The expression and characteristic of circPIP5K1A were separately analyzed by RT-qPCR, nucleic acid electrophoresis, RNase R and Actinomycin D treatment. CCK-8, colony formation, EdU, transwell, TUNEL, flow cytometry, luciferase reporter, RIP and RNA pull-down assays were employed to testify the regulatory role of circPIP5K1A in GC.

Results

In current study, circPIP5K1A, featured with closed-loop structure, was proved to be highly expressed in tissues and cells of GC. Loss-of-function assays depicted that silencing circPIP5K1A suppressed GC development. Follow-up mechanism tests unveiled that circPIP5K1A bound with miR-376c-3p and inhibition of miR-376c-3p reversed circPIP5K1A downregulation-mediated effect on GC progression. Additionally, ZNF146 was verified to be the downstream molecule of circPIP5K1A/miR-376c-3p axis in modulating GC progression.

Conclusions

circPIP5K1A stimulates GC progression by sponging miR-376c-3p to upregulate ZNF146 expression.
Appendix
Available only for authorised users
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Metadata
Title
CircPIP5K1A facilitates gastric cancer progression via miR-376c-3p/ZNF146 axis
Authors
Yan Ma
Xiliang Cong
Yiyun Zhang
Xin Yin
Ziyu Zhu
Yingwei Xue
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-1122-5

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