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Langenbeck's Archives of Surgery

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01-01-2008 | Original Article

Gas-related impact of pneumoperitoneum on systemic wound healing

Authors: R. Rosch, K. Junge, M. Binnebösel, N. Mirgartz, U. Klinge, V. Schumpelick

Published in: Langenbeck's Archives of Surgery | Issue 1/2008

Abstract

Introduction

The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen.

Materials and methods

Dorsal skin incisions were performed in 54 male Sprague–Dawley rats. A CO₂ or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds.

Results

Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO₂ group.

Conclusion

Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO₂.

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Title: Gas-related impact of pneumoperitoneum on systemic wound healing
Authors: R. Rosch
K. Junge
M. Binnebösel
N. Mirgartz
U. Klinge
V. Schumpelick
Publication date: 01-01-2008
Publisher: Springer-Verlag
Published in: Langenbeck's Archives of Surgery / Issue 1/2008
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-006-0136-3

At a glance: The STEP trials

Springer Medicine

Gas-related impact of pneumoperitoneum on systemic wound healing

Abstract

Introduction

Materials and methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction

Materials and methods

Results

Conclusion

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