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Nutrition & Metabolism
Published in: Nutrition & Metabolism 1/2010

Open Access 01-12-2010 | Research

Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver

Authors: Maznah Ismail, Ghanya Al-Naqeeb, Wan Abd Azizbin Mamat, Zalinah Ahmad

Published in: Nutrition & Metabolism | Issue 1/2010

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Abstract

Background

Gamma-oryzanol (OR), a phytosteryl ferulate mixture extracted from rice bran oil, has a wide spectrum of biological activities in particular, it has antioxidant properties.

Methods

The regulatory effect of gamma-oryzanol rich fraction (ORF) extracted and fractionated from rice bran using supercritical fluid extraction (SFE) in comparison with commercially available OR on 14 antioxidant and oxidative stress related genes was determined in rat liver. Rats were subjected to a swimming exercise program for 10 weeks to induce stress and were further treated with either ORF at 125, 250 and 500 mg/kg or OR at 100 mg/kg in emulsion forms for the last 5 weeks of the swimming program being carried out. The GenomeLab Genetic Analysis System (GeXPS) was used to study the multiplex gene expression of the selected genes.

Results

Upon comparison of RNA expression levels between the stressed and untreated group (PC) and the unstressed and untreated group (NC), seven genes were found to be down-regulated, while seven genes were up-regulated in PC group compared to NC group. Further treatment of stressed rats with ORF at different doses and OR resulted in up-regulation of 10 genes and down regulation of four genes compared to the PC group.

Conclusions

Gamma-oryzanol rich fraction showed potential antioxidant activity greater than OR in the regulation of antioxidants and oxidative stress gene markers.
Appendix
Available only for authorised users
Literature
1.
Saunders RM: Rice bran: composition and potential food sources. Food Rev Int. 1985, 13: 465-495. 10.1080/87559128509540780.CrossRef
2.
Xu Z, Godber JS: Purification and identification of components of γ-oryzanol in rice bran oil. J Agric Food Chem. 1999, 47: 2724-2728. 10.1021/jf981175j.CrossRef
3.
Lloyd BJ, Siebenmorgen TJ, Beers KW: Effects of commercial processing on antioxidants in rice bran. Cereal Chem. 2000, 77: 551-555. 10.1094/CCHEM.2000.77.5.551.CrossRef
4.
Cicero AFG, Gaddi A: Rice bran oil and γ-oryzanol in the treatment of hyperlipoproteinemias and other conditions. Phytother Res. 2001, 15: 277-289. 10.1002/ptr.907.CrossRef
5.
Kim JS, Han D, Moon KD, Rhee JS: Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci Biotechnol Biochem. 1995, 59: 822-826. 10.1271/bbb.59.822.CrossRef
6.
Hiramitsu T, Armstrong D: Preventive effect of antioxidants on lipid peroxidation in the retina. Ophtalmic Res. 1991, 23: 196-203. 10.1159/000267103.CrossRef
7.
Vaqueroe B, Simándi HG, Daood A, Sawinsky J: Recovery of pigments from Origanum majorana L. by extraction with supercritical carbon dioxide. J AGR FOOD CHEM. 2002, 50: 2297-2301. 10.1021/jf0112872.CrossRef
8.
Perretti G, Miniati E, Montanari L, Fantozzi P: Improving the value of rice by-products by SFE. J Supercrit Fluids. 2003, 26: 63-71. 10.1016/S0896-8446(02)00247-4.CrossRef
9.
Hui EKH, Rubenstein LZ: Promoting Physical Activity and Exercise in Older Adults. JAMDA. 2006, 7: 310-314.
10.
JI LL: Oxidative stress during exercise: Implication of antioxidant nutrients. Free Radic Biol Med. 1995, 18: 1079-1086. 10.1016/0891-5849(94)00212-3.CrossRef
11.
Mastaloudis A, Leonard SW, Traber MG: Oxidative stress in athletes during extreme endurance exercise. Free Radic Biol Med. 2001, 31: 911-922. 10.1016/S0891-5849(01)00667-0.CrossRef
12.
Bejma J, Ramires P, Ji LL: Free radical generation and oxidative stress with aging and exercise: differential effects in the myocardium and liver. Acta Physiol Scand. 2000, 169: 343-351. 10.1046/j.1365-201x.2000.00745.x.CrossRef
13.
Greenen D, Buttrick P, Scheuer J: Cardiovascular and hormonal responses to swimming and running in the rat. J Appl Physiol. 1988, 65: 116-123.
14.
Tan N, Morimoto K, Sugiura Morimoto TA, Murakami N: Effects of running training on the blood glucose and lactate in rats during rest and swimming. Physiol Behav. 1992, 51: 927-931. 10.1016/0031-9384(92)90072-A.CrossRef
15.
Halliwell B: Mechanisms involved in the generation of free radicals. Pathol Biol (Paris). 1996, 44: 6-13.
16.
Suri S S, Fenniri H, Sinhg B: Nanotechnology-based drug delivery systems. JOM-J OCCUP MED. 2007, 2: 16-21. 10.1186/1745-6673-2-16.CrossRef
17.
Konishi M, Tabata Y, Kariya M, Hosseinkhani H, Suzuki A, Fukuhara K, Takakura K, Fujii S: In vivo anti tumor effect of dual release of cisplatin and adriamycin from biodegradable gelatin hydrogel. J Controlled Release. 2005, 103: 7-19. 10.1016/j.jconrel.2004.11.014.CrossRef
18.
Subramani K, Hosseinkhani H, Khraisat A, Hosseinkhani M, Pathak Y: Targeting Nanoparticles as Drug Delivery Systems for Cancer Treatment. Current Nanoscience. 2009, 5: 135-140. 10.2174/157341309788185406.CrossRef
19.
Uno T, Kazui T, Suzuki Y, Hashimoto H, Suzuki K, Muhammad BA: Pharmacokinetic advantages of a newly developed tacrolimus oil-in-water type emulsion via enteral route. Lipids. 1999, 34: 249-254. 10.1007/s11745-999-0360-1.CrossRef
20.
Kramer Y, Dijkstra H, Bast H: Control of physical exercise of rats in a swimming basin. Physiol Behav. 1993, 53: 271-276. 10.1016/0031-9384(93)90204-S.CrossRef
21.
Venditti P, Meo DPL: Antioxidant, tissue damage and endurance in trained and untrained young male rats. Arch Biochem Biophys. 1996, 331: 63-68. 10.1006/abbi.1996.0283.CrossRef
22.
Tharp GD, Carson WH: Emotionally changes in rats following chronic exercise. Med Sci Sports. 1975, 7: 123-126.
23.
Shang F, Taylor A: Oxidative stress and recovery from oxidative stress are associated with altered ubiquitin conjugating and proteolytic activities in bovine lens epithelial cells. Biochem J. 1995, 307: 297-303.CrossRef
24.
Wang T, Chao A, Huang H, Lai C: Proteomics identification of stress-induced phosphoprotein (STIP1) as a potential biomarker for human ovarian cancer. J Clinical Oncology. 2008, 26: 16533-16541.
25.
Meyer M, Schreck R, Baeuerle PA: H2O2 and antioxidants have opposite effects on activation of NF-kappa B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. EMBO J. 1993, 12: 2005-2015.
26.
Wang X, Martindale JL, Liu Y: The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival. Biochem J. 1998, 333: 291-300.CrossRef
27.
Recalcati S, Tacchini L, Alberghini A, Conte D, Cairo G: Oxidative stress-mediated down-regulation of rat hydroxyacid oxidase 1, a liver-specific peroxisomal enzyme. Hepatology. 2003, 38: 1159-1166. 10.1053/jhep.2003.50417.CrossRef
28.
Espiritu DJ, Mazzone T: Oxidative Stress Regulates Adipocyte Apolipoprotein E and Suppresses Its Expression in Obesity. Diabetes. 2008, 57: 2992-2998. 10.2337/db08-0592.CrossRef
29.
Andrews GK: Regulation of metallothionein gene expression by oxidative stress and metal ions. Biochem Pharmacol. 2000, 59: 95-104. 10.1016/S0006-2952(99)00301-9.CrossRef
30.
Dalton TP, Bittel D, Andrews GK: Reversible activation of mouse metal response element-binding transcription factor 1 DNA binding involves zinc interaction with the zinc finger domain. Mol Cell Biol. 1997, 17: 2781-2789.
31.
Nath R, Kumar D, Li T, Singal PK: Metallothioneins, oxidative stress and the cardiovascular system. Toxicology. 2000, 155: 17-26. 10.1016/S0300-483X(00)00273-0.CrossRef
32.
Sagara Y, Schubert D: The Activation of Metabotropic Glutamate Receptors Protects Nerve Cells from Oxidative Stress. J Neuroscience. 1998, 18: 6662-6671.
33.
Trotti D, Danbolt NC, Volterra A: Glutamate transporters are oxidant-vulnerable: a molecular link between oxidative and excitotoxic neurodegeneration?. Trends Pharmacol Sci. 1998, 19: 328-334. 10.1016/S0165-6147(98)01230-9.CrossRef
34.
Abdel-Aal ESM, Hucl P: A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal Chemistry. 1999, 76: 350-354. 10.1094/CCHEM.1999.76.3.350.CrossRef
35.
Fridovich I: Biological effects of the superoxide radical. Arch Biochem Biophys. 1986, 24: 1-11. 10.1016/0003-9861(86)90526-6.CrossRef
36.
Awika JM, Rooney LW, Wu X, Prior RL, Zevallos LC: Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J Agric Food Chem. 2003, 51: 6657-6662. 10.1021/jf034790i.CrossRef
37.
Rukmini C, Raghuram TC: Nutritional and biochemical aspects of the hypolipidemic action of rice bran oil: a review. J American College of Nutrition. 1991, 10: 593-601.CrossRef
38.
Iqbal S, Bhanger MI, Anwar F: Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chemistry. 2005, 93: 265-272. 10.1016/j.foodchem.2004.09.024.CrossRef
39.
Yeong HL, Storkson J, Michael P: Inhibition of Benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research. 1990, 50: 1097-1101.
40.
Hosseinkhani H, Hosseinkhani M, Khademhosseini A: Emerging applications of hydrogels and microscale technologies in drug discovery. Drug Discovery. 2006, 1: 32-34.
41.
Hosseinkhani H, Hosseinkhani M: Biodegradable Polymer-Metal Complexes for Gene and Drug Delivery. Current Drug Safety. 2009, 4: 79-83. 10.2174/157488609787354477.CrossRef
42.
Hosseinkhania H, Aoyamaa T, Ogawab O, Tabataa Y: Tumor targeting of gene expression through metal-coordinated conjugation with dextran. Journal of Controlled Release. 2003, 88: 297-312. 10.1016/S0168-3659(02)00425-X.CrossRef
43.
Konishia M, Tabatab Y, Kariyaa M, Hosseinkhani H, Suzukia A, Fukuharaa K, Mandaia M, Takakuraa K, Fujii S: In vivo anti-tumor effect of dual release of cisplatin and adriamycin from biodegradable gelatin hydrogel. Journal of Controlled Release. 2005, 103: 7-19. 10.1016/j.jconrel.2004.11.014.CrossRef
44.
Yoo HS, Lee LE, Chung H, Kwon IC, Seo Young Jeong SY: Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery. Journal of Controlled Release. 2005, 103: 235-243. 10.1016/j.jconrel.2004.11.033.CrossRef
45.
Hosseinkhani H, Kushibiki T, Matsumoto K, Nakamura T, Taba Y: Enhanced suppression of tumor growth using a combination of NK4 plasmid DNA-PEG engrafted cationized dextran complex and ultrasound irradiation. Cancer Gene Therapy. 2006, 13: 479-489. 10.1038/sj.cgt.7700918.CrossRef
Metadata
Title
Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver
Authors
Maznah Ismail
Ghanya Al-Naqeeb
Wan Abd Azizbin Mamat
Zalinah Ahmad
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Nutrition & Metabolism / Issue 1/2010
Electronic ISSN: 1743-7075
DOI
https://doi.org/10.1186/1743-7075-7-23

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