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01-11-2016

Galanin receptor 2-neuropeptide Y Y1 receptor interactions in the dentate gyrus are related with antidepressant-like effects

Authors: Manuel Narváez, Dasiel O. Borroto-Escuela, Carmelo Millón, Belén Gago, Antonio Flores-Burgess, Luis Santín, Kjell Fuxe, José Angel Narváez, Zaida Díaz-Cabiale

Published in: Brain Structure and Function | Issue 8/2016

Abstract

Galanin (GAL) and the NPYY1 agonist play a role in mood regulation and both neuropeptides interact in several central functions. The present study examined the interaction between Galanin receptor 2 (GALR2) and Neuropeptide Y Y1 receptor (NPYY1R) in the dentate gyrus (DG) of the Hippocampus in relation to depression-like behavior. Using receptor autoradiography, in situ hybridization and in situ proximity ligation assay an interaction between GALR and NPYY1R was demonstrated in the DG probably involving the formation of GALR2-NPYY1R heteroreceptor complexes. These complexes were specifically observed in the polymorphic and subgranular subregions of the DG, where both receptors were found to colocalize. Moreover, this GALR2/NPYY1R interaction was linked to an enhancement of the antidepressive-like behavior mediated by NPYY1R in the forced swimming test. Specific cells populations within DG subregions may be involved in this behavioral effect since the coactivation of GALR2 and NPYY1R enhances the NPYY1R-mediated reduction in the number of c-Fos immunoreactive nuclei in the polymorphic region. These results indicate that GALR2/NPYY1R interactions can provide a novel integrative mechanism in DG in depression-related behavior and may give the basis for the development of drugs targeting GALR2/NPYY1R heteroreceptor complexes in the DG of the hippocampus for the treatment of depression.

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Title: Galanin receptor 2-neuropeptide Y Y1 receptor interactions in the dentate gyrus are related with antidepressant-like effects
Authors: Manuel Narváez
Dasiel O. Borroto-Escuela
Carmelo Millón
Belén Gago
Antonio Flores-Burgess
Luis Santín
Kjell Fuxe
José Angel Narváez
Zaida Díaz-Cabiale
Publication date: 01-11-2016
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 8/2016
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-015-1153-1

At a glance: The STEP trials

Springer Medicine

Galanin receptor 2-neuropeptide Y Y1 receptor interactions in the dentate gyrus are related with antidepressant-like effects

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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