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Open Access 01-12-2015 | Research

Further studies on the biological activity of hazelnut allergens

Authors: F. Blanc, H. Bernard, S. Ah-Leung, L. Przybylski-Nicaise, P. Stahl Skov, A. Purohit, F. de Blay, B. Ballmer-Weber, P. Fritsche, M. Fernandez Rivas, I. Reig, A. Sinaniotis, E. Vassilopoulou, K. Hoffmann-Sommergruber, S. Vieths, N. Rigby, C. Mills, K. Adel-Patient

Published in: Clinical and Translational Allergy | Issue 1/2015

Abstract

Background

Sensitization to hazelnut allergens vary depending on the geographic origin and age of the patients. The objective of this study was to further investigate the allergenic activity of hazelnut allergens using sera from patients recruited in various European regions and presenting different sensitization patterns to hazelnut proteins.

Methods

Natural Cor a 11 and Cor a 9 were purified from hazelnut whereas Cor a 1 and Cor a 8 were produced as recombinant proteins (rCor a 1.04 and rCor a 8). Sera from hazelnut allergic patients were collected in France (n = 5), Switzerland (n = 2), Greece (n = 11) and Spain (n = 3), within the Europrevall project. Total and allergen-specific IgE were quantified by enzyme allergosorbent test and IgE immunoblot were performed using pooled sera from birch-pollen endemic region or from Greece. Histamine Release (HR) assays were performed with stripped basophils passively sensitized with individual sera and challenged by a hazelnut extract or the different hazelnut allergens.

Results

As previously described, hazelnut allergic patients from Mediterranean countries are mainly sensitized to the nsLTP Cor a 8 whereas patients from France and Switzerland are sensitized to pollen-related allergens. Interestingly, an intermediate profile was evidenced in patients from Madrid. Hazelnut 7S globulin (Cor a 11) and 11S globulin (Cor a 9) were found to be minor allergens, recognized only by patients from Mediterranean countries. The biologic activity of the 4 tested allergens, analysed by HR assay, further confirmed the sensitization patterns, but also demonstrated the very high elicitation potency of Cor a 8.

Conclusions

This work, extending previously published researches, represents a step towards the better understanding of the complexity of hazelnut allergy and provides new data on the biological activity of hazelnut allergens and extracts.

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Title: Further studies on the biological activity of hazelnut allergens
Authors: F. Blanc
H. Bernard
S. Ah-Leung
L. Przybylski-Nicaise
P. Stahl Skov
A. Purohit
F. de Blay
B. Ballmer-Weber
P. Fritsche
M. Fernandez Rivas
I. Reig
A. Sinaniotis
E. Vassilopoulou
K. Hoffmann-Sommergruber
S. Vieths
N. Rigby
C. Mills
K. Adel-Patient
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Clinical and Translational Allergy / Issue 1/2015
Electronic ISSN: 2045-7022
DOI: https://doi.org/10.1186/s13601-015-0066-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Further studies on the biological activity of hazelnut allergens

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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