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Journal of Ovarian Research

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Open Access 01-12-2008 | Research

Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep

Authors: Kate E Ryan, Claire Glister, Pat Lonergan, Finian Martin, Phil G Knight, Alexander CO Evans

Published in: Journal of Ovarian Research | Issue 1/2008

Abstract

Background

The intracellular signalling mechanisms that regulate ovarian follicle development are unclear; however, we have recently shown differences in the Akt and Erk signalling pathways in dominant compared to subordinate follicles. The aim of this study was to investigate the effects of inhibiting Akt and Erk phosphorylation on IGF- and gonadotropin- stimulated granulosa and theca cell function in vitro, and on follicle development in vivo.

Methods

Bovine granulosa and theca cells were cultured for six days and stimulated with FSH and/or IGF, or LH in combination with PD98059 (Erk inhibitor) and/or LY294002 (Akt inhibitor) and their effect on cell number and hormone secretion (estradiol, activin-A, inhibin-A, follistatin, progesterone and androstenedione) determined. In addition, ovarian follicles were treated in vivo with PD98059 and/or LY294002 in ewes on Day 3 of the cycle and follicles were recovered 48 hours later.

Results

We have shown that gonadotropin- and IGF-stimulated hormone production by granulosa and theca cells is reduced by treatment with PD98059 and LY294002 in vitro. Furthermore, treatment with PD98059 and LY294002 reduced follicle growth and oestradiol production in vivo.

Conclusion

These results demonstrate an important functional role for the Akt and Erk signalling pathways in follicle function, growth and development.

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Title: Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep
Authors: Kate E Ryan
Claire Glister
Pat Lonergan
Finian Martin
Phil G Knight
Alexander CO Evans
Publication date: 01-12-2008
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2008
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-1-2

At a glance: The STEP trials

Springer Medicine

Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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