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01-04-2013 | Original article

Functional human Th17 clones with WT1-specific helper activity

Authors: Sho Tachino, Fumihiro Fujiki, Yoshihiro Oka, Akihiro Tsuboi, Soyoko Morimoto, Yu-Hung Lin, Taichi Tamanaka, Kenta Kondo, Hiroko Nakajima, Sumiyuki Nishida, Naoki Hosen, Yusuke Oji, Atsushi Kumanogoh, Haruo Sugiyama

Published in: Cancer Immunology, Immunotherapy | Issue 4/2013

Abstract

Th17 plays important roles in the pathogenesis of various inflammatory and autoimmune diseases. Although the importance of Th17 in tumor immunity has also been suggested, precise roles of tumor-associated antigen-specific Th17 still remain poorly understood, especially in humans. We previously identified WT1₃₃₂, a 16-mer helper epitope derived from tumor-associated antigen Wilms’ tumor gene 1 (WT1) product, and WT1₃₃₂-specific Th1 clones were established. In the present study, WT1-specific Th17 clones were established by the stimulation of peripheral blood mononuclear cells with the WT1₃₃₂ helper peptide under human Th17-polarizing conditions. The WT1-specific Th17 clone exhibited the helper function for proliferation of conventional CD4⁺ T cells in the antigenic stimulation-specific manner. This is the first report of establishment of functional Th17 clones with both antigen (WT1₃₃₂) specificity and antigen-specific helper activity. Th17 clones established here and the method to establish antigen-specific Th17 clones should be a useful tool to further analyze the roles of human Th17 in tumor immunity.

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Title: Functional human Th17 clones with WT1-specific helper activity
Authors: Sho Tachino
Fumihiro Fujiki
Yoshihiro Oka
Akihiro Tsuboi
Soyoko Morimoto
Yu-Hung Lin
Taichi Tamanaka
Kenta Kondo
Hiroko Nakajima
Sumiyuki Nishida
Naoki Hosen
Yusuke Oji
Atsushi Kumanogoh
Haruo Sugiyama
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: Cancer Immunology, Immunotherapy / Issue 4/2013
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-012-1385-3

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