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01-08-2014 | Laboratory Investigation

FRK suppresses the proliferation of human glioma cells by inhibiting cyclin D1 nuclear accumulation

Authors: Lei Hua, Ming Zhu, Xu Song, Jun Wang, Zhen Fang, Chunting Zhang, Qiong Shi, Wenjian Zhan, Lei Wang, Qingming Meng, Xiuping Zhou, Rutong Yu

Published in: Journal of Neuro-Oncology | Issue 1/2014

Abstract

The Fyn related kinase (FRK) is a noteworthy member of the Src non-receptor tyrosine kinase family for its distinctive tumor suppressive function. Recently, we have shown that FRK plays a protective role against the progression of glioma by suppressing cell migration and invasion. However, it is unclear whether the cell growth of glioma is also regulated by FRK and by which mechanism FRK alters its specific biological functions. In the current study, we found that FRK over-expression significantly suppressed the proliferation of glioma cells. In contrast, FRK knockdown by siRNA promoted glioma cell growth. In addition, FRK over-expression caused G₁ phase arrest as well as apoptosis of glioma cells. Further investigation disclosed that FRK-induced G1 arrest was accompanied by down-regulation of hyperphosphorylated retinoblastoma protein (pRb), which led to the consequent suppression of E2F1. More importantly, we found that over-expression of FRK inhibited proper cyclin D1 accumulation in the nucleus of proliferating cells. Taken together, our results demonstrate a combined mechanism for the anti-proliferative effects of FRK by inhibiting cyclin D1 nucleus accumulation and pRb phosphorylation in glioma cells.

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Title: FRK suppresses the proliferation of human glioma cells by inhibiting cyclin D1 nuclear accumulation
Authors: Lei Hua
Ming Zhu
Xu Song
Jun Wang
Zhen Fang
Chunting Zhang
Qiong Shi
Wenjian Zhan
Lei Wang
Qingming Meng
Xiuping Zhou
Rutong Yu
Publication date: 01-08-2014
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 1/2014
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-014-1461-y

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FRK suppresses the proliferation of human glioma cells by inhibiting cyclin D1 nuclear accumulation

Abstract

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Springer Medicine

Abstract

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