Top

Reproductive Biology and Endocrinology

Published in:

Open Access 01-12-2011 | Research

Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells

Authors: Shuo Huang, Ping Zhao, Liying Yang, Yuan Chen, Jie Yan, Enkui Duan, Jie Qiao

Published in: Reproductive Biology and Endocrinology | Issue 1/2011

Abstract

Background

Recent evidence from rodent ovaries has demonstrated expression of fractalkine and the existence of fractalkine receptor, and showed that there is a significant increase in steroidogenesis in response to fractalkine, yet the role of fractalkine and CX3CR1 in the human ovary is still unknown. This study aimed to determine the expression levels of fractalkine and CX3CR1 in the human ovary and to investigate their roles in sexual hormone biosynthesis by human luteinising granulosa cells. This is the first detailed report of fractalkine and CX3CR1 expression and function in the human ovary.

Methods

Fractalkine and CX3CR1 expression levels were measured by immunohistochemistry using ovarian tissue from pathological specimens from five individuals. Granulosa cells were obtained from patients during IVF treatment. They were cultured and treated with increasing doses of hCG with or without fractalkine. Media were collected to detect estradiol and progesterone by chemiluminescence. StAR, 3-βHSD and CYP11A expression were determined in granulosa cells treated with or without fractalkine by real-time RT-PCR.

Results

Fractalkine and CX3CR1 were expressed in the human ovary and in luteinising granulosa cells. However, fractalkine expression was stronger in luteinising granulosa cells. Treatment with fractalkine augmented hCG stimulation of progesterone production in a dose-dependent manner with concomitant increases in transcript levels for key steroidogenic enzymes (StAR, 3-βHSD and CYP11A) but had no effect on estradiol biosynthesis(P < 0.05).

Conclusions

Fractalkine and CX3CR1 were found to express in human ovary and luteinising granulosa cells. Fractalkine can increase the biosynthesis of progesterone in a dose-dependent manner by enhancing transcript levels of key steroidogenic enzymes.

Available only for authorised users

Richards JS: Hormonal control of gene expression in the ovary. Endocr Rev. 1994, 15: 725-751.CrossRefPubMed

Hsueh AJ, Adashi EY, Jones PB, Welsh TH: Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocr Rev. 1984, 5: 76-127. 10.1210/edrv-5-1-76.CrossRefPubMed

Christenson LK, Devoto L: Cholesterol transport and steroidogenesis by the corpus luteum. Reproductive Biology and Endocrinology. 2003, 1: 90-10.1186/1477-7827-1-90.PubMedCentralCrossRefPubMed

Zhao P, De A, Hu Z, Li J, Mulders SM, Gelpke MDS, Duan EK, Hsueh AJ: Gonadotropin Stimulation of Ovarian Fractalkine Expression and Fractalkine Augmentation of Progesterone Biosynthesis by Luteinizing Granulosa Cells. Endocrinology. 2008, 149 (6): 2782-2789. 10.1210/en.2007-1662.PubMedCentralCrossRefPubMed

Zlotnik A, Yoshie O, Chemokines: a new classification system and their role in immunity. Immunity. 2000, 12: 121-127. 10.1016/S1074-7613(00)80165-X.CrossRefPubMed

Bazan JF, Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, Greaves DR, Zlotnik A, Schall TJ: A new class of membrane-bound chemokine with a CX3C motif. Nature. 1997, 385: 640-644. 10.1038/385640a0.CrossRefPubMed

Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki M, Takagi S, Nomiyama H, Schall TJ, Yoshie O: Identification and molecular charaeterization for fractalkine receptor CX3CRI , which mediates both leukocyte migration and adhesion. Cell. 1997, 91 (4): 521-530. 10.1016/S0092-8674(00)80438-9.CrossRefPubMed

Pan Y, Lloyd C, Zhou H, Dolich S, Deeds J, Gonzalo JA, Vath J, Gosselin M, Ma J, Dussault B, Woolf E, Alperin G, Culpepper J, Gutierrez-Ramos JC, Gearing D: Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature. 1997, 387 (6633): 611-617. 10.1038/42491.CrossRefPubMed

Jung S, Aliberti J, Graemmel P, Sunshine MJ, Kreutzberg GW, Sher A, Littman DR: Analysis of fractalkine receptor CX3CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol Cell Biol. 2000, 20: 4106-4114. 10.1128/MCB.20.11.4106-4114.2000.PubMedCentralCrossRefPubMed

10.

Harrison JK, Jiang Y, Chen S, Xia Y, Maciejewski D, McNamara RK, Streit WJ, Salafranca MN, Adhikari S, Thompson DA: Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1 expressing microglia. Proc Natl Acad Sci USA. 1998, 95 (18): 10896-10901. 10.1073/pnas.95.18.10896.PubMedCentralCrossRefPubMed

11.

Vitale S, Schmid-Alliana A, Breuil V, Pomeranz M, Millet MA, Rossi B, Schmid-Antomarchi H: Soluble fractalkine prevents monocyte chemoattractant protein-1-induced monocyte migration via inhibition of stress-activated protein kinase 2/p38 and matrix metalloproteinase activities. J Immunol. 2004, 172: 585-592.CrossRefPubMed

12.

Fong AM, Robinson LA, Steeber DA, Tedder TF, Yoshie O, Imai T, Patel DD: Fractalkine and CX3CR1 mediate a novel mechanism of leukocyte capture, firm adhesion, and activation under physiologic flow. J Exp Med. 1998, 188: 1413-1419. 10.1084/jem.188.8.1413.PubMedCentralCrossRefPubMed

13.

Wong KH, Negishi H, Adashi EY: Expression, hormonal regulation, and cyclic variation of chemokines in rat ovary: key determinants of the intraovarian residence of representatives of the white blood cell series. Endocrinology. 2002, 143: 784-791. 10.1210/en.143.3.784.CrossRefPubMed

14.

Ben-Shlomo I, Rauch R, Avsian-Kretchmer O, Hsueh AJW: Matching receptome genes with their ligands for surveying paracrine/autocrine signaling systems. Mol Endocrinol. 2007, 21: 2009-2014. 10.1210/me.2007-0087.CrossRefPubMed

15.

Miceli F, Tropea A, Minici F, Navarra P, Lanzone A, Apa R: Interleukin-1_ stimulates progesterone production by in vitro human luteal cells: evidence of a mediatory role of prostaglandins. J Clin Endocrinol Metab. 2003, 88: 2690-2694. 10.1210/jc.2002-020819.CrossRefPubMed

16.

Goffin V, Binart N, Touraine P, Kelly PA: Prolactin: the new biology of an old hormone. Annu Rev Physiol. 2002, 64: 47-67. 10.1146/annurev.physiol.64.081501.131049.CrossRefPubMed

17.

Gras S, Hannibal J, Fahrenkrug J: Pituitary adenylate cyclase-activating polypeptide is an auto/paracrine stimulator of acute progesterone accumulation and subsequent luteinization in cultured periovulatory granulosa/lutein cells. Endocrinology. 1999, 140 (5): 2199-2205. 10.1210/en.140.5.2199.PubMed

18.

Ronen-Fuhrmann T, Timberg R, King SR, Hales KH, Hales DB, Stocco DM, Orly J: Spatio-temporal expression patterns of steroidogenic acute regulatory protein (StAR) during follicular development in the rat ovary. Endocrinology. 1998, 139 (1): 303-315. 10.1210/en.139.1.303.PubMed

19.

Gasic S, Bodenburg Y, Nagamani M, Green A, Urban RJ: Troglitazone inhibits progesterone production in porcine granulose cells. Endocrinology. 1998, 139 (12): 4962-4966. 10.1210/en.139.12.4962.PubMed

20.

Storbeck KH, Swart P, Swart AC: Cytochrome P450 side-chain cleavage: Insights gained from homology modeling. Mol Cell Endocrinol. 2007, 265-266. 65-70

21.

Baker ME: Hydroxysteroid dehydrogenases: ancient and modern regulators of adrenal and sex steroid action. Mol Cell Endocrinol. 2001, 175 (1-2): 1-4. 10.1016/S0303-7207(01)00399-9.CrossRefPubMed

Title: Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells
Authors: Shuo Huang
Ping Zhao
Liying Yang
Yuan Chen
Jie Yan
Enkui Duan
Jie Qiao
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2011
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/1477-7827-9-95

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2011

The effects of pre-pregnancy body mass index and gestational weight gain on perinatal outcomes in Korean women: a retrospective cohort study

Should a single blastocyst transfer policy be a clinical decision or should it depend on the embryological evaluation on day 3?

Structural and histological characterization of oviductal magnum and lectin-binding patterns in Gallus domesticus

Sympathetic nerve activity in normal and cystic follicles from isolated bovine ovary: local effect of beta-adrenergic stimulation on steroid secretion

Diet-induced obesity in rats leads to a decrease in sperm motility

Bivariate analysis of basal serum anti-Müllerian hormone measurements and human blastocyst development after IVF