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01-06-2013 | Research Article

Fluorouracil selectively enriches stem-like cells in the lung adenocarcinoma cell line SPC

Authors: Mu-mu Shi, Yan-lei Xiong, Xin-shan Jia, Xin Li, Li Zhang, Xiao-lei Li, En-Hua Wang

Published in: Tumor Biology | Issue 3/2013

Abstract

Most adult stem cells are in the G0 or quiescent phase of the cell cycle and account for only a small percentage of the cells in the tissue. Thus, isolation of stem cells from tissues for further study represents a major challenge. This study sought to enrich cancer stem cells and explore cancer stem-like cell clones using 5-fluorouracil (5-FU) in the lung adenocarcinoma cell line, SPC. Proliferation inhibition was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, according to which half maximal inhibitory concentration values were calculated. Expression levels of stem cell markers after treatment with 5-FU were examined using immunofluorescence and Western blotting. Additionally, side population (SP) cells were sorted using FACS. Properties of SP cells were evaluated by using Transwell, colony-forming assays, and tumor formation experiments. 5-FU greatly inhibits proliferation, especially of cells in S phase. SP cells possess greater invasive potential, higher clone-forming potential, and greater tumor-forming ability than non-SP cells. Treatment with 5-FU enriches the SP cells with stem cell properties in human lung adenocarcinoma cell lines.

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Title: Fluorouracil selectively enriches stem-like cells in the lung adenocarcinoma cell line SPC
Authors: Mu-mu Shi
Yan-lei Xiong
Xin-shan Jia
Xin Li
Li Zhang
Xiao-lei Li
En-Hua Wang
Publication date: 01-06-2013
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 3/2013
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-013-0675-5

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