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01-06-2019 | Short Communication

Fluoro-Jade B histofluorescence staining detects dentate granule cell death after repeated five-minute transient global cerebral ischemia

Authors: Ji Hyeon Ahn, Taek Geun Ohk, Dae Won Kim, Hyunjung Kim, Minah Song, Tae-Kyeong Lee, Jae-Chul Lee, Go Eun Yang, Myoung Cheol Shin, Jun Hwi Cho, Soo Young Choi, Moo-Ho Won, Joon Ha Park

Published in: Metabolic Brain Disease | Issue 3/2019

Abstract

Transient global cerebral ischemia (tGCI)-induced neuronal damage is variable according to its duration and degree. There are many studies on the damage or death of pyramidal cells of the hippocampus proper (CA1–3) in rodent models of tGCI. However, studies on the death of granule cells in the hippocampal dentate gyrus (DG) following tGCI have not yet been addressed. In this study, we examined the damage/death of granule cells in the gerbil DG at 5 days after various durations (5, 10, and 15 min) of single tGCI and repeated tGCI (two 5-min tGCI with 1-h interval) using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B (F-J B) histofluorescence staining. Neuronal death was observed only in the polymorphic layer in all single tGCI-operated groups. However, in the repeated tGCI-operated group, massive neuronal death was observed in the granule cell layer as well as in the polymorphic layer by using F-J B histofluorescence staining. In addition, microgliosis in the DG was significantly increased in the repeated tGCI-operated group compared to the 15-min tGCI-operated group. Taken together, these findings indicate that repeated brief tGCI causes granule cell death in the DG which could not occur by a longer duration of single tGCI.

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Title: Fluoro-Jade B histofluorescence staining detects dentate granule cell death after repeated five-minute transient global cerebral ischemia
Authors: Ji Hyeon Ahn
Taek Geun Ohk
Dae Won Kim
Hyunjung Kim
Minah Song
Tae-Kyeong Lee
Jae-Chul Lee
Go Eun Yang
Myoung Cheol Shin
Jun Hwi Cho
Soo Young Choi
Moo-Ho Won
Joon Ha Park
Publication date: 01-06-2019
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 3/2019
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-019-00404-5

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Fluoro-Jade B histofluorescence staining detects dentate granule cell death after repeated five-minute transient global cerebral ischemia

Abstract

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Abstract

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