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01-03-2015 | Original Paper

Filamentous structures in skeletal muscle: anchors for the subsarcolemmal space

Authors: Astrid Feinisa Khairani, Yuki Tajika, Maiko Takahashi, Hitoshi Ueno, Tohru Murakami, Arifin Soenggono, Hiroshi Yorifuji

Published in: Medical Molecular Morphology | Issue 1/2015

Abstract

In skeletal muscle fibers, intermediate filaments and actin filaments provide structural support to the myofibrils and the sarcolemma. For many years, it was poorly understood from ultrastructural observations that how these filamentous structures were kept anchored. The present study was conducted to determine the architecture of filamentous anchoring structures in the subsarcolemmal space and the intermyofibrils. The diaphragms (Dp) of adult wild type and mdx mice (mdx is a model for Duchenne muscular dystrophy) were subjected to tension applied perpendicular to the long axis of the muscle fibers, with or without treatment with 1 % Triton X-100 or 0.03 % saponin. These experiments were conducted to confirm the presence and integrity of the filamentous anchoring structures. Transmission electron microscopy revealed that these structures provide firm transverse connections between the sarcolemma and peripheral myofibrils. Most of the filamentous structures appeared to be inserted into subsarcolemmal densities, forming anchoring connections between the sarcolemma and peripheral myofibrils. In some cases, actin filaments were found to run longitudinally in the subsarcolemmal space to connect to the sarcolemma or in some cases to connect to the intermyofibrils as elongated thin filaments. These filamentous anchoring structures were less common in the mdx Dp. Our data suggest that the transverse and longitudinal filamentous structures form an anchoring system in the subsarcolemmal space and the intermyofibrils.

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Title: Filamentous structures in skeletal muscle: anchors for the subsarcolemmal space
Authors: Astrid Feinisa Khairani
Yuki Tajika
Maiko Takahashi
Hitoshi Ueno
Tohru Murakami
Arifin Soenggono
Hiroshi Yorifuji
Publication date: 01-03-2015
Publisher: Springer Japan
Published in: Medical Molecular Morphology / Issue 1/2015
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI: https://doi.org/10.1007/s00795-014-0070-3

At a glance: The STEP trials

Springer Medicine

Filamentous structures in skeletal muscle: anchors for the subsarcolemmal space

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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