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BMC Health Services Research
Published in: BMC Health Services Research 1/2013

Open Access 01-12-2013 | Research article

Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

Authors: Donato Koyalta, Mohammad-Ali Jenabian, Barou Djouater, Noël Djemadji-Oudjeil, Francois-Xavier Mbopi-Keou, Angélique Ndjoyi-Mbiguino, Laurent Bélec

Published in: BMC Health Services Research | Issue 1/2013

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Abstract

Background

Field and community evaluation of the routine usage of CD4 T counting platforms is essential in resource-poor countries for efficient and cost-effective monitoring of HIV-infected adults and children attending health care centers.

Methods

We herein addressed the principal issues raised by the implementation of the single-platform, volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) in 8 community HIV monitoring laboratories of different levels throughout Chad. This is a country with particularly difficult conditions, both in terms of climate and vast geographical territory, making the decentralization of the therapeutic management of HIV-infected patients challenging.

Results

The routine usage of the Auto40 flow cytometers for a period of 5 years (2008–2013) confirms the reliability and robustness of the analyzer for community-based CD4 T cell enumeration in terms of both absolute numbers and percentages to enable accurate monitoring of HIV-infected adults and children. However, our observations suggest that the Auto40 mini flow cytometer is not suitable for all laboratories as it is oversized and ultimately very expensive.

Conclusion

The Chad experience with the Auto40 flow cytometer suggests that its usage in resource-limited settings should be mainly reserved to reference (level 1) or district (level 2) laboratories, rather than to laboratories of health care centres (level 3).
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Metadata
Title
Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry
Authors
Donato Koyalta
Mohammad-Ali Jenabian
Barou Djouater
Noël Djemadji-Oudjeil
Francois-Xavier Mbopi-Keou
Angélique Ndjoyi-Mbiguino
Laurent Bélec
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Health Services Research / Issue 1/2013
Electronic ISSN: 1472-6963
DOI
https://doi.org/10.1186/1472-6963-13-373

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