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01-08-2008 | Original Article

Fibrinolytic system of the armadillo Chaetophractus villosus (Xenarthra, Dasypodidae)

Authors: Juan Tentoni, Nélida Nora Polini, Emma Beatriz Casanave

Published in: Comparative Clinical Pathology | Issue 3/2008

Abstract

The fibrinolytic mechanism in the armadillo Chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae) quite unknown until now was studied. Results were compared with those corresponding to healthy adult human beings. Whole blood lysis time and diluted blood lysis time were not detectable in armadillos. Euglobulin clot lysis time and plasminogen activity (Plg) were lower than in healthy humans. We established the presence of the fibrinolytic system in Ch. villosus through the measurement of fibrin fibrinogen degradation products. The activity of the plasminogen activator inhibitor was two to four times greater than in healthy humans. The activity of the alpha 2 anti-plasmin (α2AP) was similar and displaced toward lower values. The Plg/α2AP relation was lower. The results obtained suggest that Ch. villosus has a hypercoagulable and hypofibrinolytic profile. Our findings are not only the first contribution to elucidate the physiology of the fibrinolytic system in Xenarthra but also contribute to develop an animal model for studies in haemostasis and thrombosis.

Amiral J, Grosley M, Mimilla F, Plassart V, Chambrette B (1990) Monoclonal antibodies to different neo-epitopes on fibrinogen and fibrin degradation products. Blood Coagul. Fibrinolysis 1(4–5):447–452PubMed

Bidla G, Lindgren M, Theopold U, Dushay MS (2005) Hemolymph coagulation and phenoloxidase in Drosophila larvae. Dev Comp Immunol 29(8):669–679PubMedCrossRef

Casanave EB, Bermúdez PM, Polini NN (2005) Haemostatic mechanisms of the armadillo Chaetophractus villosus (Xenarthra, Dasypodidae). Comp Clin Path 13(4):171–175CrossRef

Casanave EB, Bermúdez PM, Polini NN (2006) Principal coagulation factors and natural anticoagulants in the armadillo Chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae). Comp Clin Path 14(4):210–216CrossRef

Clauss A (1957) Rapid physiological coagulation method in determination of fibrinogen. Acta Haematol 17(4):237–246PubMed

Conard J (1976) Plasma plasminogen activator-clot lysis assay techniques. In: Davidson JF, Samama MM, Desnoyers PC (eds) Progress in chemical fibrinolysis and thrombolysis, vol 2. Raven, New York. USA, pp 15–16

Contant G, Nicham F, Martinoli J (1992) Determination of plasminogen activator inhibitor (PAI) by a new venom based assay. Fibrinolysis 3:85–86CrossRef

Declerck PJ, Alessi MC, Verstreken M, Kruithof EK, Juhan-Vague I, Collen D (1998) Measurement of plasminogen activator inhibitor 1 in biologic fluids with a murine monoclonal antibody-based enzyme-linked immunosorbent assay. Blood 71(1):220–225

Diaz GB and Ojeda RA (2000) Libro rojo de mamíferos amenazados de la Argentina. Sociedad Argentina para el Estudio de los Mamíferos, SAREM, pp. 42

Dobrovolsky AB, Titaeva EV (2002) The fibrinolysis system: regulation of activity and physiologic functions of its main components. Biochemistry 67(1):99–108PubMed

Duboscq C, Quintana I, Bassilotta E, Bergonzelli GE, Porterie P, Sassetti B, Haedo AS, Wainsztein N, Kruithof EKO, Kordich L (1997) Plasminogen: an important hemostatic parameter in septic patients. Thromb Haemost 77:1090–1095PubMed

El-Sayed MS, Ali ZE, Ahmadizad S (2004) Exercise and training effects on blood haemostasis in health and disease. Sports Med 34:181–200PubMedCrossRef

Emerson JD, Strenio J (1983) Boxplots and batch comparison. In: Hoaglin DC, Mosteller F, Tukey JW (eds) Understanding robust and exploratory data analysis. Wiley, New York, 3:58–96

Esmon CT (2004) Crosstalk between inflammation and thrombosis. Maturitas 47:305–314PubMedCrossRef

Fearnley GR, Balmforth G, Fearnley E (1957) Evidence of a diurnal fibrinolytic rhythm; with a simple method of measuring natural fibrinolysis. Clin Sci 16:645–650PubMed

Greenberg CS, Orthner CL (1999) Chapter 24: Blood coagulation and fibrinolysis. In: Wintrobe’s clinical hematology 10th edn. Williams and Wilkins, Maryland. USA, pp 684–764

Johnson SI, McMichael M, White G (2006) Heatstroke in small animal medicine: a clinical practice review. J Vet Emerg Crit Care 16:112–119CrossRef

Juhan-Vague I, Hans M (2003) From fibrinogen to fibrin and its dissolution. Bull Acad Natl Med 187(1):69–84PubMed

Kowalski E, Kopec M, Niewiarowski S (1959) An evaluation of the euglobulin method for the determination of fibrinolysis. J Clin Path 12:215–218PubMedCrossRef

Kubalek S, Mischke R, Fehr M (2002) Investigations on blood coagulation in the green iguana (Iguana iguana). J Vet Med A 49:210–216CrossRef

Lewis JH, Doyle AP (1964) Coagulation, protein and cellular studies on armadillo blood. Comp Biochem Physiol 12:61–66PubMedCrossRef

Menoud PA, Sappino N, Boudal-Khoshbeen M, Vassalli JD, Sappino AP (1996) The kidney is a major site of alpha(2)-antiplasmin production. J Clin Invest 97:2478–2484PubMedCrossRef

Munster AM, Olsen AK, Bladbjerg EM (2002) Usefulness of human coagulation and fibrinolysis assays in domestic pigs. Comp Med 52:39–43PubMed

Perkins SL (1999) Appendix A: Normal blood and bone marrow values in humans. In: Wintrobe’s clinical hematology, 10th edn. Williams and Wilkins, Maryland. USA, pp 2738–2748

Polini NN, Camina RE, Casanave EB (1999) Morphological and morphometric study of blood leucocytes from Chaetophractus villosus (Mammalia, Dasypodidae). Comp Haematol Int 9:162–167CrossRef

Ravanat C, Freund M, Dol F, Cadroy Y, Roussi J, Incardona F, Maffrand JP, Boneu B, Drouet L, Legrand C, Herbert J-M, Cazenave JP (1995) Cross-reactivity of human molecular markers for detection of prethrombotic states in various animal species. Blood Coagul Fibrinolysis 6:446–455PubMedCrossRef

Roberts M, Newman L, Peterson G (1982) The management and reproduction of the hairy armadillo Chaetophractus villosus, at the National Zoological Park. Int Zoo Yearb 22:1875–1894CrossRef

Snedecor GW, Cochran WG (1980) Statistical methods. 7th edn. The Iowa State University Press, Ames, USA

Stötzer KE, Amiral J, Spanuth E (1988) Neue Methoden zur spezifischen Bestimmung von Fibrinspaltprodukten (DDimere). Lab Med 12:51–59

Teger-Nilsson AC, Friberger P, Gyzander E (1977) Determination of a new rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate. Scand J Clin Lab Invest 37:403–409PubMedCrossRef

Theopold U, Li D, Fabbri M, Scherfer C, Schmidt O (2002) The coagulation of insect hemolymph. Cell Mol Life Sci 59(2):363–372PubMedCrossRef

Vaughan DE, Declerck PJ (2003) Chapter 6: Regulation of fibrinolysis. In: Thrombosis and hemorrhage. 3rd edn. Williams and Wilkins, Baltimore, USA, pp 105–119

Williams WJ, Lichtman MA, Beutler E, Kipps TJ (2005) In: Marbán SL (ed) Manual de hematología, 6th edn. Madrid, España

Winer BJ, Brown DR, Michels KM (1991) Statistical principles in experimental design, 3rd edn. McGraw-Hill, New York, USA

Title: Fibrinolytic system of the armadillo Chaetophractus villosus (Xenarthra, Dasypodidae)
Authors: Juan Tentoni
Nélida Nora Polini
Emma Beatriz Casanave
Publication date: 01-08-2008
Publisher: Springer-Verlag
Published in: Comparative Clinical Pathology / Issue 3/2008
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-008-0721-7

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Fibrinolytic system of the armadillo Chaetophractus villosus (Xenarthra, Dasypodidae)

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