Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Medical Molecular Morphology
Published in: Medical Molecular Morphology 2/2019

Open Access 01-06-2019 | Original Paper

FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice

Authors: Natsuko Kakudo, Naoki Morimoto, Takeshi Ogawa, Shigeru Taketani, Kenji Kusumoto

Published in: Medical Molecular Morphology | Issue 2/2019

Login to get access

Abstract

Fibroblast growth factor (FGF)-2 induces mitogenesis, angiogenesis and adipogenesis. In this study, the adipogenesis-inducing effects of FGF-2 combined with bilayer artificial dermis in mice were evaluated. FGF-2-impregnated bilayer artificial dermis composed of collagen matrix, PELNAC (Gunze Corp., Osaka, Japan) was implanted subcutaneously into the thoracic region of mice. At 1, 2, 3, and 4 weeks, samples were collected for H&E staining, von Willebrand factor immunostaining, and perilipin immunostaining to examine adipose tissue localization and angiogenesis. The collagen matrix-implanted group without the addition of FGF-2 was prepared as a control. At 2 weeks after the implantation of FGF-2 combined with dermal substitutes, adipocytes appeared in the collagen fibers. At 3–4 weeks, a fat pad was generated with neovascularization. The thickness of the fat pad had significantly increased at 2, 3, and 4 weeks. The remaining collagen was decreased by absorption over time. In the control group, no fat pad was newly formed. This study has identified a promising method to enhance adipogenic effects in the murine subcutis, representing a potential technique for soft tissue reconstruction.
Literature
1.
Kakudo N, Morimoto N, Ogawa T, Kusumoto K (2014) Potential of adipose-derived stem cells for regeneration medicine: clinical application and usefulness of fat grafting. J Stem Cells Res Ther 4:1000204
2.
Langer R (2007) Tissue engineering: perspectives, challenges, and future directions. Tissue Eng 13:1–2CrossRefPubMed
3.
Gospodarowicz D, Ferrara N, Schweigerer L, Neufeld G (1987) Structural characterization and biological functions of fibroblast growth factor. Endocr Rev 8:95–114CrossRefPubMed
4.
Powers CJ, McLeskey SW, Wellstein A (2000) Fibroblast growth factors, their receptors and signaling. Endocr Relat Cancer 7:165–197CrossRefPubMed
5.
Kakudo N, Shimotsuma A, Kusumoto K (2007) Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells. Biochem Biophys Res Commun 359:239–244CrossRefPubMed
6.
Suzuki S, Matsuda K, Maruguchi T, Nishimura Y, Ikada Y (1995) Further applications of “bilayer artificial skin”. Br J Plast Surg 48:222–229CrossRefPubMed
7.
Eto H, Kato H, Suga H, Aoi N, Doi K, Kuno S, Yoshimura K (2012) The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstruct Surg 129:1081–1092CrossRef
8.
Kakudo N, Morimoto N, Ogawa T, Hihara M, Notodihardjo PV, Matsui M, Tabata Y, Kusumoto K (2017) Angiogenic effect of platelet-rich plasma combined with gelatin hydrogel granules injected into murine subcutis. J Tissue Eng Regener Med 11:1941–1948CrossRef
9.
Notodihardjo PV, Morimoto N, Kakudo N, Matsui M, Sakamoto M, Liem PH, Suzuki K, Tabata Y, Kusumoto K (2015) Gelatin hydrogel impregnated with platelet-rich plasma releasate promotes angiogenesis and wound healing in murine model. J Artif Org 18:64–71CrossRef
10.
11.
Zaragosi LE, Ailhaud G, Dani C (2006) Autocrine fibroblast growth factor 2 signaling is critical for self-renewal of human multipotent adipose-derived stem cells. Stem Cells 24:2412–2419CrossRefPubMed
12.
Harish V, Raymond AP, Maitz PK (2014) Reconstruction of soft tissue necrosis secondary to cryoglobulinaemia. J Plast Reconstruct Aesthet Surg 67:1151–1154CrossRef
13.
Suzuki S, Morimoto N, Yamawaki S, Fujitaka J, Kawai K (2013) A case of giant naevus followed up for 22 years after treatment with artificial dermis. J Plast Reconstruct Aesthet Surg 66:e229–e233CrossRef
14.
Eo S, Kim Y, Cho S (2011) Vacuum-assisted closure improves the incorporation of artificial dermis in soft tissue defects: terudermis((R)) and pelnac((R)). Int Wound J 8:261–267CrossRefPubMed
15.
Suzuki S, Kawai K, Ashoori F, Morimoto N, Nishimura Y, Ikada Y (2000) Long-term follow-up study of artificial dermis composed of outer silicone layer and inner collagen sponge. Br J Plast Surg 53:659–666CrossRefPubMed
16.
Mizuno H, Takeda A, Uchinuma E (1999) Creation of an acellular dermal matrix from frozen skin. Aesthet Plast Surg 23:316–322CrossRef
17.
Kawaguchi N, Toriyama K, Nicodemou-Lena E, Inou K, Torii S, Kitagawa Y (1998) De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor. Proc Natl Acad Sci USA 95:1062–1066CrossRefPubMed
18.
Hiraoka Y, Yamashiro H, Yasuda K, Kimura Y, Inamoto T, Tabata Y (2006) In situ regeneration of adipose tissue in rat fat pad by combining a collagen scaffold with gelatin microspheres containing basic fibroblast growth factor. Tissue Eng 12:1475–1487CrossRefPubMed
19.
Kimura Y, Inamoto T, Tabata Y (2010) Adipose tissue formation in collagen scaffolds with different biodegradabilities. J Biomater Sci Polym Ed 21:463–476CrossRefPubMed
20.
Kanazawa S, Fujiwara T, Matsuzaki S, Shingaki K, Taniguchi M, Miyata S, Tohyama M, Sakai Y, Yano K, Hosokawa K, Kubo T (2010) bFGF regulates PI3-kinase-Rac1-JNK pathway and promotes fibroblast migration in wound healing. PLoS One 5:e12228CrossRefPubMedPubMedCentral
Metadata
Title
FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice
Authors
Natsuko Kakudo
Naoki Morimoto
Takeshi Ogawa
Shigeru Taketani
Kenji Kusumoto
Publication date
01-06-2019
Publisher
Springer Japan
Published in
Medical Molecular Morphology / Issue 2/2019
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI
https://doi.org/10.1007/s00795-018-0203-1

Other articles of this Issue 2/2019

Medical Molecular Morphology 2/2019 Go to the issue

Original Paper

Phosphorylated HER3 and FITC-labeled trastuzumab immunohistochemistry in patients with HER2-positive breast cancer treated with adjuvant trastuzumab

Review

Protective role of heme oxygenase-1 in fatty liver ischemia–reperfusion injury

Original Paper

Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer

Original Paper

Knockdown of long non-coding RNA PCAT1 in glioma stem cells promotes radiation sensitivity

Original Paper

Evaluation of immunohistochemical expression of survivin and its correlation with −31G/C gene polymorphism in colorectal cancer

Original Paper

Enhanced expression of nidogen 1 around the nest of basal cell carcinoma compared with that around squamous cell carcinoma