Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Diabetology & Metabolic Syndrome
Published in: Diabetology & Metabolic Syndrome 1/2020

01-12-2020 | Fertility | Research

The potential therapeutic effects of Lactobacillus plantarum and inulin on serum and testicular reproductive markers in diabetic male rats

Authors: Marziyeh Rahimiyan-Heravan, Leila Roshangar, Pouran Karimi, Safa Sefidgari-Abrasi, Mohammad Morshedi, Maryam Saghafi-Asl, Khadijeh Bavafa-Valenlia

Published in: Diabetology & Metabolic Syndrome | Issue 1/2020

Login to get access

Abstract

Background

It is well established that diminished reproductive health is one of the notable long-term outcomes of type 2 diabetes mellitus (T2DM), especially among males. Due to the global increasing rate of T2DM and infertility, we aimed to investigate the impact of Lactobacillus plantarum (L. plantarum), inulin, and their combinatory supplementation on fertility markers as well as testicular kisspeptin and androgen receptor (AR)’s expression in diabetic male rats.

Methods

Thirty-five Male Wistar rats with Streptozotocin-induced T2DM were supplemented with L. plantarum, inulin, or their combination for 8 weeks. At the end-point, the animals were sacrificed and serum, testicular, and seminal parameters were studied.

Results

Administration of L. plantarum and inulin in diabetic male rats improved sperm motility and viability (P < 0.001, both) as well as testicular tissue development via increasing leydig cell number, testicular spermatid count, and diameter of seminiferous tubules (P < 0.001, all). Testicular expression of Kisspeptin was elevated by inulin supplementation (P = 0.01). L. plantarum administration increased testicular AR expression (P = 0.01). The expression of Kisspeptin showed a remarkable correlation with fertility markers (P < 0.001).

Conclusion

Supplementation with either L. plantarum, inulin, or their combination can prevent infertility caused by T2DM in male rats via improving testicular kisspeptin and AR expression, leydig cell count, and effectively increasing epididymal sperm motility and viability.
Literature
1.
Adedara IA, et al. Garcinia kola seed ameliorates renal, hepatic, and testicular oxidative damage in streptozotocin-induced diabetic rats. Pharm Biol. 2015;53(5):695–704.PubMed
2.
Afifi M, et al. Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin-induced diabetic rat testes. Biomed Res Int. 2015;2015:153573.PubMedPubMedCentral
3.
Al-Asmakh M, et al. The gut microbiota and developmental programming of the testis in mice. PLoS ONE. 2014;9(8):e103809.PubMedPubMedCentral
4.
Anjum S, et al. Localization of gonadotropin-releasing hormone (GnRH), gonadotropin-inhibitory hormone (GnIH), kisspeptin and GnRH receptor and their possible roles in testicular activities from birth to senescence in mice. J Exp Zool A. 2012;317(10):630–44.
5.
Ballester J, et al. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl. 2004;25(5):706–19.PubMed
6.
Bested AC, et al. Intestinal microbiota, probiotics and mental health: from Metchnikoff to modern advances: part I–autointoxication revisited. Gut Pathog. 2013;5(1):5.PubMedPubMedCentral
7.
8.
Chang C, et al. Infertility with defective spermatogenesis and hypotestosteronemia in male mice lacking the androgen receptor in Sertoli cells. Proc Natl Acad Sci. 2004;101(18):6876–81.PubMedPubMedCentral
9.
Clarke H, et al. Comprehensive Review on Kisspeptin and Its Role in Reproductive Disorders. Endocrinol Metab (Seoul). 2015;30(2):124–41.
10.
Dai Z, et al. Amino acid metabolism in intestinal bacteria and its potential implications for mammalian reproduction. Mol Hum Reprod. 2015;21(5):389–409.PubMed
11.
De Vries MC, et al. Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract. Int Dairy J. 2006;16(9):1018–28.
12.
Dhewa T, et al. Development of freeze dried synbiotic formulation using a probiotic strain of Lactobacillus plantarum. J Food Sci Technol. 2014;51(1):83–9.PubMed
13.
Du Plessis SS, et al. The effect of obesity on sperm disorders and male infertility. Nat Rev Urol. 2010;7(3):153–61.PubMed
14.
Duckett RJ, et al. The effects of gonadotropin-releasing hormone immunization and recombinant follicle-stimulating hormone on the Leydig cell and macrophage populations of the adult rat testis. J Androl. 1997;18(4):417–23.PubMed
15.
George JT, et al. Hypothesis: kisspeptin mediates male hypogonadism in obesity and type 2 diabetes. Neuroendocrinology. 2010;91(4):302–7.PubMed
16.
Gioacchini G, et al. Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic. Reprod Fertil Dev. 2013;25(8):1115–25.PubMed
17.
Havel PJ, et al. Marked and rapid decreases of circulating leptin in streptozotocin diabetic rats: reversal by insulin. Am J Physiol. 1998;274(5):R1482–91.PubMed
18.
Hill C, et al. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Am J Physiol Endocrinol Metab. 2014;11(8):506.
19.
Hill JW, et al. Hypothalamic pathways linking energy balance and reproduction. Am J Physiol Endocrinol Metab. 2008;294(5):E827–32.PubMed
20.
Hosseinifard E-S, et al. The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions. Eur J Nutr. 2019;58(8):3361–75.PubMed
21.
Hutkins RW, et al. Prebiotics: why definitions matter. Curr Opin Biotechnol. 2016;37:1–7.PubMed
22.
Ibrahim HA, et al. Selenium-enriched probiotics improves murine male fertility compromised by high fat diet. Biol Trace Elem Res. 2012;147(1–3):251–60.PubMed
23.
Jungwirth A, et al. European Association of Urology guidelines on Male Infertility: the 2012 update. Eur Urol. 2012;62(2):324–32.PubMed
24.
Kent Christensen A, Peacock KC. Increase in Leydig cell number in testes of adult rats treated chronically with an excess of human chorionic gonadotropin. Biol Reprod. 1980;22(2):383–91.
25.
Kerr JB, Sharpe RM. Stimulatory effect of follicle-stimulating hormone on rat Leydig cells. A morphometric and ultrastructural study. Cell Tissue Res. 1985;239(2):405–15.PubMed
26.
Kleniewska P, et al. The influence of probiotic Lactobacillus casei in combination with prebiotic inulin on the antioxidant capacity of human plasma. Oxid Med Cell Longevity. 2016;2016(2016):1340903.
27.
La Vignera S, et al. Diabetes mellitus and sperm parameters. J Androl. 2012;33(2):145–53.PubMed
28.
Mauvais-Jarvis F. Estrogen and androgen receptors: regulators of fuel homeostasis and emerging targets for diabetes and obesity. Trends Endocrinol Metab. 2011;22(1):24–33.PubMed
29.
Morshedi M, et al. Beneficial psychological effects of novel psychobiotics in diabetic rats: the interaction among the gut, blood, and amygdala. J Nutr Biochem. 2018;57:145–52.PubMed
30.
Morton GJ, et al. Leptin regulates insulin sensitivity via phosphatidylinositol-3-OH kinase signaling in mediobasal hypothalamic neurons. Cell Metab. 2005;2(6):411–20.PubMed
31.
Nazzaro F, et al. Biochemical traits, survival and biological properties of the probiotic Lactobacillus plantarum grown in the presence of prebiotic inulin and pectin as energy source. Pharmaceuticals. 2012;5(5):481–92.PubMedPubMedCentral
32.
Nudmamud-Thanoi S, et al. Changes of sperm quality and hormone receptors in the rat testis after exposure to methamphetamine. Drug Chem Toxicol. 2016;39(4):432–8.PubMed
33.
Poutahidis T, et al. Probiotic microbes sustain youthful serum testosterone levels and testicular size in aging mice. PLoS ONE. 2014;9(1):e84877.PubMedPubMedCentral
34.
Prabhu A, et al. Expression of aromatase, androgen and estrogen receptors in peripheral target tissues in diabetes. Steroids. 2010;75(11):779–87.PubMedPubMedCentral
35.
Rey R, et al. Testicular dysgenesis does not affect expression of anti-müllerian hormone by Sertoli cells in premeiotic seminiferous tubules. Am J Pathol. 1996;148(5):1689.PubMedPubMedCentral
36.
Ricci G, et al. Diabetic rat testes: morphological and functional alterations. Andrologia. 2009;41(6):361–8.PubMed
37.
Saez-Lara MJ, et al. Effects of probiotics and synbiotics on obesity, insulin resistance syndrome, type 2 diabetes and non-alcoholic fatty liver disease: a review of human clinical trials. Int J Mol Sci. 2016;17(6):928.PubMedCentral
38.
Salehi S, et al. Developmental and endocrine regulation of kisspeptin expression in mouse Leydig cells. Endocrinology. 2015;156(4):1514–22.PubMedPubMedCentral
39.
Sefidgari-Abrasi S, et al. Lactobacillus plantarum and inulin: therapeutic agents to enhance cardiac ob receptor expression and suppress cardiac apoptosis in type 2 diabetic rats. J Diabetes Res. 2020;2020(2020):4745389.PubMedPubMedCentral
40.
41.
Soto C. Lactobacillus plantarum as source of conjugated linoleic acid: effect of pH, incubation Temperature and inulin incorporation. J Biochem Technol. 2013;5(1):649–53.
42.
Takemura N, et al. Inulin prolongs survival of intragastrically administered Lactobacillus plantarum No. 14 in the gut of mice fed a high-fat diet. J Nutr. 2010;140(11):1963–9.PubMed
43.
Tarr AJ, et al. The prebiotics 3′ Sialyllactose and 6′ Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: evidence for effects on the gut–brain axis. Brain Behav Immun. 2015;50:166–77.PubMedPubMedCentral
44.
Teerds K, et al. Functional relationship between obesity and male reproduction: from humans to animal models. Human Reprod Update. 2011;17(5):667–83.
45.
Tonucci LB, et al. Clinical application of probiotics in diabetes mellitus: therapeutics and new perspectives. Crit Rev Food Sci Nutr. 2015.
46.
Valenlia KB, et al. Beneficial impacts of Lactobacillus plantarum and inulin on hypothalamic levels of insulin, leptin, and oxidative markers in diabetic rats. J Funct Foods. 2018;46:529–37.
47.
Wang JY, et al. Kisspeptin expression in mouse Leydig cells correlates with age. J Chin Med Assoc. 2015;78(4):249–57.PubMed
48.
Wang Y. Prebiotics: present and future in food science and technology. Food Res Int. 2009;42(1):8–12.
49.
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes care. 2004;27(5):1047–53.
50.
Xu Y, et al. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model. Biochem Biophys Res Commun. 2014;450(1):87–92.PubMed
Metadata
Title
The potential therapeutic effects of Lactobacillus plantarum and inulin on serum and testicular reproductive markers in diabetic male rats
Authors
Marziyeh Rahimiyan-Heravan
Leila Roshangar
Pouran Karimi
Safa Sefidgari-Abrasi
Mohammad Morshedi
Maryam Saghafi-Asl
Khadijeh Bavafa-Valenlia
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Diabetology & Metabolic Syndrome / Issue 1/2020
Electronic ISSN: 1758-5996
DOI
https://doi.org/10.1186/s13098-020-00560-0

Other articles of this Issue 1/2020

Diabetology & Metabolic Syndrome 1/2020 Go to the issue

Research

Association between metabolic syndrome and prognosis of breast cancer: a meta-analysis of follow-up studies

Research

The effects of taurine supplementation on oxidative stress indices and inflammation biomarkers in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled trial

Research

Therapeutic effects of different doses of prebiotic (isolated from Saccharomyces cerevisiae) in comparison to n-3 supplement on glycemic control, lipid profiles and immunological response in diabetic rats

Research

The relationship between QT interval indices with cardiac autonomic neuropathy in diabetic patients: a case control study

Research

Effect of supplementation with vitamins D3 and K2 on undercarboxylated osteocalcin and insulin serum levels in patients with type 2 diabetes mellitus: a randomized, double-blind, clinical trial

Research

High anxiety and depressive symptoms in partners of type 1 diabetes persons in a sample of the Brazilian population
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Image Credits