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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 3/2016

01-06-2016 | Research Article

Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

Authors: Kazumichi Kawakubo, Shunsuke Ohnishi, Yutaka Hatanaka, Kanako C. Hatanaka, Hidetaka Hosono, Yoshimasa Kubota, Mako Kamiya, Masaki Kuwatani, Hiroshi Kawakami, Yasuteru Urano, Naoya Sakamoto

Published in: Molecular Imaging and Biology | Issue 3/2016

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Abstract

Purpose

Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT).

Procedures

We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min.

Results

GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05).

Conclusions

Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
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Metadata
Title
Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study
Authors
Kazumichi Kawakubo
Shunsuke Ohnishi
Yutaka Hatanaka
Kanako C. Hatanaka
Hidetaka Hosono
Yoshimasa Kubota
Mako Kamiya
Masaki Kuwatani
Hiroshi Kawakami
Yasuteru Urano
Naoya Sakamoto
Publication date
01-06-2016
Publisher
Springer US
Published in
Molecular Imaging and Biology / Issue 3/2016
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-015-0898-5

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