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01-10-2019 | Fatty Liver | Original Article

Hepatoprotective and cytotoxicity properties of aqueous extract of Glycyrrhiza glabra in Wistar rats fed with high-fat diet

Authors: Samaneh Goorani, Hassan Morovvati, Niloofar Seydi, Maryam Almasi, Amir Amiri-Paryan, Farzaneh Nazari, Mohammad Mahdi Zangeneh, Akram Zangeneh

Published in: Comparative Clinical Pathology | Issue 5/2019

Abstract

The recent studies have shown the effect of ethnomedicinal plants on the treatment of fatty liver disease. The purpose of the experiment was to survey the remedial activity of Glycyrrhiza glabra aqueous extract on high-fat diet-induced hepatotoxicity in rats. The synthesized extract had great cell viability dose-dependently, and this method was found to be non-toxic for synthesizing G. glabra. In the study, a total of 10 rats were chosen as the negative control, and 40 rats were treated with a high-fat diet for 4 months. Then, the animals were randomly divided into five subgroups, including negative healthy control, untreated negative control, and three groups receiving the G. glabra aqueous extract at 20, 60, and 180 mg/kg concentrations. After 2 months, the rats were sacrificed, and blood and liver samples of them collected. Different groups of G. glabra could significantly (p ≤ 0.05) decrease the increased concentrations of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, and glucose, and enhance HDL, total protein, albumin, SOD, and CAT as compared to the untreated group. Also, G. glabra reduced the degree of hepatic steatosis as compared to the untreated group. It appears that the G. glabra aqueous extract can treat fatty liver disease in rats without any side effect.

Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684CrossRef

Angulo P, Lindor KD (2002) Non-alcoholic fatty liver disease. J Gastroenterol Hepatol 17(Suppl1):186–190

Anil K, Jyotsna D (2012) Review on Glycyrrhiza glabra. J Pharm Sci Innov 1(2):1–4

Arulmozhi V, Pandian K, Mirunalini S (2013) Ellagic acid encapsulated chitosan nanoparticles for drug delivery system in human oral cancer cell line (KB). Colloids Surf B: Biointerfaces 110:313–320PubMed

Askari F, Rashidkhani B, Hekmatdoost A (2014) Cinnamon may have therapeutic benefits on lipid profile, liver enzymes, insulin resistance, and high-sensitivity C-reactive protein in nonalcoholic fatty liver disease patients. Nutr Res 34(2):143–148PubMed

Asl MN, Hosseinzadeh H (2008) Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res 22(6):709–724PubMed

Chen HJ, Kang SP, Lee IJ, Lin YL (2014) Glycyrrhetinic acid suppressed NF-kappaB activation in TNF-alpha-induced hepatocytes. J Agric Food Chem 62(3):618–625PubMed

Comar KM, Sterling RK (2005) Review article: drug therapy for non-alcoholic fatty liver disease. Aliment Pharmacol Ther 23(2):207–215

Day CP (2006) From fat to inflammation. Gastroenterol 130:207–210

Day CP (2011) Non-alcoholic fatty liver disease: a massive problem. Clin Med 11(2):176–178

Dehghani-Tafti L, Shariatpanahi SM, Mahdavi-Damghani M, Javadi B (2017) Traditional Persian topical medications for gastrointestinal diseases. Iran J Basic Med Sci 20(3):222–241

Ebrahimi-Mameghani M, Aliashrafi S, Javadzadeh Y, AsghariJafarabadi M (2014) The effect of Chlorella vulgaris supplementation on liver enzymes, serum glucose and lipid profile in patients with non-alcoholic fatty liver disease. Health Promot Perspect 4(1):107–115PubMedPubMedCentral

Fan JG, Farrell GC (2009) Epidemiology of non-alcoholic fatty liver disease in China. J Hepatol 50(1):204–210PubMed

Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A (2018) Stereological assessment of nephroprotective effects of Trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. Int J Morphol 36(2):750–757

Flora K, Hahn M, Rosen H, Benner K (1998) Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol 93(2):139–143

Ganz M, Csak T, Szabo G (2014) High fat diet feeding results in gender specific steatohepatitis and inflammasome activation. World J Gastroenterol 20:8525–8534PubMedPubMedCentral

Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of Methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263

Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hosseini MS (2018) Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol https://doi.org/10.1007/s00580-018-2866-3

Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM (2019) The aqueous extract of Allium saralicum 1 R.M. Fritsch effectively treat induced anemia. Experimental study on Wistar rats. Orient Pharm Exp Med https://doi.org/10.1007/s13596-019-00361-5

Haga Y, Kanda T, Sasaki R, Nakamura M, Nakamoto S, Yokosuka O (2015) Nonalcoholic fatty liver disease and hepatic cirrhosis: comparison with viral hepatitis-associated steatosis. World J Gastroenterol 21:12989–12995PubMedPubMedCentral

Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463

Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organomet Chem 32(9):e4458

Hashemi SJ, Hajiani E, Sardabi EH (2009) A placebo-controlled trial of silymarin in patients with nonalcoholic fatty liver disease. Hepat Mon 9(4):265–270

Huo HZ, Wang B, Liang YK, Bao YY, Gu Y (2011) Hepatoprotective and antioxidant effects of licorice extract against CCl₄-induced oxidative damage in rats. Int J Mol Sci 12(10):6529–6543PubMedPubMedCentral

Jacobs BP, Dennehy C, Ramirez G, Sapp J, Lawrence VA (2002) Milk thistle for the treatment of liver disease a systematic review and meta-analysis. Am J Med 113(6):506–515PubMed

Jung JC, Lee YH, Kim SH, Kim KJ, Kim KM, Oh S, Jung YS (2016) Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease. BMC Complement Altern Med 16:19. https://doi.org/10.1186/s12906-016-0997-0 CrossRefPubMedPubMedCentral

Kashkooli RI, Najafi SS, Sharif F, Hamedi A, Asl MKH, Kalyani MN, Birjandi M (2015) The effect of Berberis Vulgaris extract on transaminase activities in non-alcoholic fatty liver disease. Hepat Mon 15(2):e25067

Laylani LAS (2016) Hepatoprotective effect of Glycyrrhiza Glabra L. extracts against carbon tetrachloride-induced acute liver damage in rats. Int J Vet Sci 1(1):1–8

Martin JP, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336PubMed

Mohammadifar M, Behnam M, Talaei SA, Khamechian T, Mehran M, Taghizadeh M (2018) Evaluation effect of Silybum Marianum, Cynara Scolymus L. and Ziziphus jujube Mill. combination extract on nonalcoholic fatty liver in rats. Iranian J Endocrinol Metab 19(6):410–418

Montoro P, Maldini M, Russo M, Postorino S, Piacente S, Pizza C (2011) Metabolic profiling of roots of liquorice (Glycyrrhiza glabra) from different geographical areas by ESI/MS/MS and determination of major metabolites by LC-ESI/MS and LC-ESI/MS/MS. J Pharm Biomed Anal 54(3):535–544PubMed

Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol https://doi.org/10.1007/s00580-018-2834-y

Nikkhajoei M, Choopani R, Tansaz M, Heydarirad G, Hashem-Dabaghian F, Sahranavard S, Saberifiroozi M, Pasalar M (2016) Herbal medicines used in treatment of nonalcoholic fatty liver disease: a mini-review. Galen Med J 5(3):107–113

Panahi Y, Ghamarchehreh ME, Beiraghdar F, Zare R, Jalalian HR, Sahebkar A (2012) Investigation of the effects of Chlorella vulgaris supplementation in patients with non-alcoholic fatty liver disease: a randomized clinical trial. Hepatogastroenterology 59:2099–2103PubMed

Sakata R, Nakamura T, Torimura T, Ueno T, Sata M (2013) Green tea with high-density catechins improves liver function and fat infiltration in non-alcoholic fatty liver disease (NAFLD) patients: a double-blind placebo-controlled study. Int J Mol Med 32(5):989–994PubMed

Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Plants 21(1):164–174

Shaker E, Mahmoud H, Mnaa S (2010) Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol 48(3):803–806PubMed

Sharafzadeh S, Alizadeh O (2012) Some medicinal plants cultivated in Iran. J Appl Pharm Sci 2(1):134–137

Shavakhi A, Torki M, Khodadoostan M, Shavakhi S (2015) Effects of cumin on nonalcoholic steatohepatitis: a double blind, randomised, controlled trial. Adv Biomed Res 4:212. https://doi.org/10.4103/2277-9175.166149 CrossRefPubMedPubMedCentral

Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl₄-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543

Tamayo C, Diamond S (2007) Review of clinical trials evaluating safety and efficacy of milk thistle (Silybum marianum [L.] Gaertn.). Integr Cancer Ther 6(2):146–157PubMed

Wong VW, Wong GL, Chan AW, Chu WC, Choi PC, Chim AM, Yiu KK, Yu J, Chan FK, Chan HL (2013) Treatment of non-alcoholic steatohepatitis with Phyllanthus urinaria: a randomized trial. J Gastroenterol Hepatol 28(1):57–62PubMed

Yin GJ, Cao LP, Xu P, Jeney G, Nakao M, Lu CP (2011) Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extract against carbon tetrachloride (CCl4)-induced hepatocyte damage in common carp (Cyprinus carpio). Fish Physiol Biochem 37(1):209–216PubMed

Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27(4):861–867

Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018b) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Bio Sci 70(4):655–664

Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018c) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl₄-induced hepatic injury in mice. Comp Clin Pathol 27:1359–1365. https://doi.org/10.1007/s00580-018-2747-9 CrossRef

Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plants 21(2):493–501

Title: Hepatoprotective and cytotoxicity properties of aqueous extract of Glycyrrhiza glabra in Wistar rats fed with high-fat diet
Authors: Samaneh Goorani
Hassan Morovvati
Niloofar Seydi
Maryam Almasi
Amir Amiri-Paryan
Farzaneh Nazari
Mohammad Mahdi Zangeneh
Akram Zangeneh
Publication date: 01-10-2019
Publisher: Springer London
Keyword: Fatty Liver
Published in: Comparative Clinical Pathology / Issue 5/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02939-6

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Hepatoprotective and cytotoxicity properties of aqueous extract of Glycyrrhiza glabra in Wistar rats fed with high-fat diet

Abstract

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Abstract

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