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Journal of Clinical Monitoring and Computing

Published in:

01-10-2013 | Review Paper

Factors affecting hemoglobin measurement

Author: Lauren Berkow

Published in: Journal of Clinical Monitoring and Computing | Issue 5/2013

Abstract

A review of the literature shows that current “standard” laboratory measurements for hemoglobin are subject to numerous factors that affect both accuracy and reliability. In addition, total hemoglobin concentration measurements are subject to numerous factors that affect the “true” hemoglobin value. This article discusses both the physiologic factors that influence hemoglobin levels and the technical aspects and variability among the different measurement methodologies currently available.

Beer-Lambert law is the linear relationship between absorbance and concentration of an absorbing species and is typically written as:

A = a(λ) × b × c

where A is the measured absorbance, a(λ) is a wavelength-dependent absorptivity coefficient, b is the path length, and c is the analyte concentration.

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Title: Factors affecting hemoglobin measurement
Author: Lauren Berkow
Publication date: 01-10-2013
Publisher: Springer Netherlands
Published in: Journal of Clinical Monitoring and Computing / Issue 5/2013
Print ISSN: 1387-1307
Electronic ISSN: 1573-2614
DOI: https://doi.org/10.1007/s10877-013-9456-3

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Factors affecting hemoglobin measurement

Abstract

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