Published in:
Open Access
01-04-2010 | Oral presentation
Facilities of a novel tissue fixation method: the HOPE-technology
Authors:
Ekkehard Vollmer, Holger Schultz, Dagmar Silvia Lang, Daniel Kähler, Florian Stellmacher, Torsten Goldmann
Published in:
Diagnostic Pathology
|
Special Issue 1/2010
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Excerpt
We introduced an alternative and novel tissue fixative and compared it to conventional formalin fixation. Besides different stains used in routine diagnostics including histochemistry as well as immunohistochemistry we focused on molecular pathology. The latter gives many obstacles when using formalin fixed paraffin embedded tissue (FFPET), and many of the modern molecular techniques can even only be achieved on fresh or frozen specimens in the past and not on FFPET. In our repertoire many common molecularbiological techniques on the nucleic acid level (RNA and/or DNA) of HOPE fixed and paraffin embedded tissues were compared to formalin fixation as well as to fresh and/or frozen material. …
