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Archives of Orthopaedic and Trauma Surgery

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01-11-2012 | Orthopaedic Surgery

Extracorporeal shock-wave therapy reduces progression of knee osteoarthritis in rabbits by reducing nitric oxide level and chondrocyte apoptosis

Authors: Zhe Zhao, Huiru Ji, Rufang Jing, Chunmei Liu, Mingbo Wang, Lei Zhai, Xiaodong Bai, Gengyan Xing

Published in: Archives of Orthopaedic and Trauma Surgery | Issue 11/2012

Abstract

Introduction

The goal for treating osteoarthritis (OA) is finding ways to decrease joint pain and dysfunction and prevent and slow the cartilage degeneration. Extracorporeal shock-wave therapy (ESWT) has been found to improve motor dysfunction and ameliorate pain with OA in animals. However, few studies have found that it can prevent and slow joint degeneration in vivo. The aim of study was to investigate the effect of ESWT on OA in rabbit.

Materials and methods

A total of 30 male New Zealand white rabbits were divided into 3 groups: control, OA induced by anterior cruciate ligament transaction (ACLT), and ALCT plus ESWT. The animals were killed at 4 and 8 weeks. Nitric oxide (NO) level was measured in the synovial cavity of knee joints, and cartilage sections were graded macroscopically by a Mankin scoring system. Chondrocyte apoptosis was investigated by flow cytometry and the expression of active caspase 3 by indirect immunohistochemistry.

Results

ESWT significantly reduced the NO level in the synovial cavity of knee joints (P < 0.05) and chondrocyte apoptosis (P < 0.05) of rabbits with OA. ESWT treatment significantly decreased the severity of cartilage lesions at both times as compared to rabbits with OA alone (P < 0.05).

Conclusion

ESWT reduced the progression of OA in rabbits. This effect may be related to decreased level of NO and is likely mediated by reduced chondrocyte apoptosis. ESWT may be a useful treatment for knee OA.

Shirai T, Kobayashi M, Nishitani K, Satake T, Kuroki H, Nakagawa Y, Nakamura T (2011) Chondroprotective effect of alendronate in a rabbit model of osteoarthritis. J Orthop Res 29(10):1572–1577. doi:10.1002/jor.21394 PubMedCrossRef

Hawker GA, Mian S, Bednis K, Stanaitis I (2011) Osteoarthritis year 2010 in review: non-pharmacologic therapy. Osteoarthr Cartil 19(4):366–374. doi:10.1016/j.joca.2011.01.021 PubMedCrossRef

Kim HA, Blanco FJ (2007) Cell death and apoptosis in osteoarthritic cartilage. Curr Drug Targets 8(2):333–345PubMedCrossRef

van der Kraan PM, van den Berg WB (2012) Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration? Osteoarthr Cartil 20(3):223–232. doi:10.1016/j.joca.2011.12.003 PubMedCrossRef

Endres S, Weiskirch M, Hinz C, Hutter F, Wilke A (2008) Extracorporeal shock-wave therapy in the treatment of pseudoarthrosis: a case report. Cases J 1(1):276. doi:10.1186/1757-1626-1-276 PubMedCrossRef

Albert JD, Meadeb J, Guggenbuhl P, Marin F, Benkalfate T, Thomazeau H, Chales G (2007) High-energy extracorporeal shock-wave therapy for calcifying tendinitis of the rotator cuff: a randomised trial. J Bone Joint Surg Br 89(3):335–341. doi:10.1302/0301-620X.89B3.18249 PubMedCrossRef

Staples MP, Forbes A, Ptasznik R, Gordon J, Buchbinder R (2008) A randomized controlled trial of extracorporeal shock wave therapy for lateral epicondylitis (tennis elbow). J Rheumatol 35(10):2038–2046PubMed

Metzner G, Dohnalek C, Aigner E (2010) High-energy Extracorporeal Shock-Wave Therapy (ESWT) for the treatment of chronic plantar fasciitis. Foot Ankle Int 31(9):790–796. doi:10.3113/FAI.2010.0790 PubMedCrossRef

Frisbie DD, Kawcak CE, McIlwraith CW (2009) Evaluation of the effect of extracorporeal shock wave treatment on experimentally induced osteoarthritis in middle carpal joints of horses. Am J Vet Res 70(4):449–454. doi:10.2460/ajvr.70.4.449 PubMedCrossRef

10.

Revenaugh MS (2005) Extracorporeal shock wave therapy for treatment of osteoarthritis in the horse: clinical applications. Vet Clin North Am Equine Pract 21(3):609–625. doi:10.1016/j.cveq.2005.09.001

11.

Ochiai N, Ohtori S, Sasho T, Nakagawa K, Takahashi K, Takahashi N, Murata R, Moriya H, Wada Y, Saisu T (2007) Extracorporeal shock wave therapy improves motor dysfunction and pain originating from knee osteoarthritis in rats. Osteoarthr Cartil 15(9):1093–1096. doi:10.1016/j.joca.2007.03.011 PubMedCrossRef

12.

Li X, Li J, Cheng K, Lin Q, Wang D, Zhang H, An H, Gao M, Chen A (2011) Effect of low-intensity pulsed ultrasound on MMP-13 and MAPKs signaling pathway in rabbit knee osteoarthritis. Cell Biochem Biophys 61(2):427–434. doi:10.1007/s12013-011-9206-4 PubMedCrossRef

13.

Wang FS, Yang KD, Kuo YR, Wang CJ, Sheen-Chen SM, Huang HC, Chen YJ (2003) Temporal and spatial expression of bone morphogenetic proteins in extracorporeal shock wave-promoted healing of segmental defect. Bone 32(4):387–396 pii: S8756328203000292PubMedCrossRef

14.

Iwase Y, Kato J, Ohtaguro K (1989) Clinical experiences of Medstone 1050 ST on extracorporeal shock wave lithotripsy. Nihon Rinsho 47(12):2777–2780PubMed

15.

Yoshioka M, Coutts RD, Amiel D, Hacker SA (1996) Characterization of a model of osteoarthritis in the rabbit knee. Osteoarthr Cartil 4(2):87–98 pii: S1063-4584(05)80318-8PubMedCrossRef

16.

Vaterlein N, Lussenhop S, Hahn M, Delling G, Meiss AL (2000) The effect of extracorporeal shock waves on joint cartilage—an in vivo study in rabbits. Arch Orthop Trauma Surg 120(7–8):403–406PubMedCrossRef

17.

Renz H, Rupp S (2009) Effects of shock waves on chondrocytes and their relevance in clinical practice. Arch Orthop Trauma Surg 129(5):641–647. doi:10.1007/s00402-008-0668-9 PubMedCrossRef

18.

Benson BM, Byron CR, Pondenis H, Stewart AA (2007) The effects of radial shock waves on the metabolism of equine cartilage explants in vitro. N Z Vet J 55(1):40–44. doi:10.1080/00480169.2007.36733 PubMedCrossRef

19.

Mayer-Wagner S, Ernst J, Maier M, Chiquet M, Joos H, Muller PE, Jansson V, Sievers B, Hausdorf J (2010) The effect of high-energy extracorporeal shock waves on hyaline cartilage of adult rats in vivo. J Orthop Res 28(8):1050–1056. doi:10.1002/jor.21074 PubMed

20.

Wang CJ, Weng LH, Ko JY, Wang JW, Chen JM, Sun YC, Yang YJ (2011) Extracorporeal shockwave shows regression of osteoarthritis of the knee in rats. J Surg Res 171(2):601–608. doi:10.1016/j.jss.2010.06.042 PubMedCrossRef

21.

Ciampa AR, de Prati AC, Amelio E, Cavalieri E, Persichini T, Colasanti M, Musci G, Marlinghaus E, Suzuki H, Mariotto S (2005) Nitric oxide mediates anti-inflammatory action of extracorporeal shock waves. FEBS Lett 579(30):6839–6845. doi:10.1016/j.febslet.2005.11.023 PubMedCrossRef

22.

Mariotto S, Cavalieri E, Amelio E, Ciampa AR, de Prati AC, Marlinghaus E, Russo S, Suzuki H (2005) Extracorporeal shock waves: from lithotripsy to anti-inflammatory action by NO production. Nitric Oxide 12(2):89–96. doi:10.1016/j.niox.2004.12.005 PubMedCrossRef

23.

Moretti B, Iannone F, Notarnicola A, Lapadula G, Moretti L, Patella V, Garofalo R (2008) Extracorporeal shock waves down-regulate the expression of interleukin-10 and tumor necrosis factor-alpha in osteoarthritic chondrocytes. BMC Musculoskelet Disord 9:16. doi:10.1186/1471-2474-9-16 PubMedCrossRef

24.

Abramson SB (2008) Nitric oxide in inflammation and pain associated with osteoarthritis. Arthr Res Ther 10(Suppl 2):S2. doi:10.1186/ar2463 CrossRef

25.

Hancock CM, Riegger-Krugh C (2008) Modulation of pain in osteoarthritis: the role of nitric oxide. Clin J Pain 24(4):353–365. doi:10.1097/AJP.0b013e31815e5418 PubMedCrossRef

26.

Li D, Wu Z, Duan Y, Hao D, Zhang X, Luo H, Chen B, Qiu G (2011) TNFalpha-mediated apoptosis in human osteoarthritic chondrocytes sensitized by PI3K-NF-kappaB inhibitor, not mTOR inhibitor. Rheumatol Int. doi:10.1007/s00296-011-1929-4

Title: Extracorporeal shock-wave therapy reduces progression of knee osteoarthritis in rabbits by reducing nitric oxide level and chondrocyte apoptosis
Authors: Zhe Zhao
Huiru Ji
Rufang Jing
Chunmei Liu
Mingbo Wang
Lei Zhai
Xiaodong Bai
Gengyan Xing
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: Archives of Orthopaedic and Trauma Surgery / Issue 11/2012
Print ISSN: 0936-8051
Electronic ISSN: 1434-3916
DOI: https://doi.org/10.1007/s00402-012-1586-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Extracorporeal shock-wave therapy reduces progression of knee osteoarthritis in rabbits by reducing nitric oxide level and chondrocyte apoptosis

Abstract

Introduction

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Materials and methods

Results

Conclusion

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