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01-10-2015 | Original Article

Expression patterns and prognostic role of transketolase-like 1 in muscle-invasive bladder cancer

Authors: Mauro Semilia, Jörg Hennenlotter, Carlo Pavone, Teresa Bischoff, Ursula Kühs, Georgios Gakis, Jens Bedke, Arnulf Stenzl, Christian Schwentner, Tilman Todenhöfer

Published in: World Journal of Urology | Issue 10/2015

Abstract

Purpose

The pentose phosphate pathway (PPP) has been shown to play an important role in the metabolism of cancer cells. The transketolase-like 1 gene (TKTL1) encodes an enzyme representing an essential component of this pathway. Its expression has been demonstrated to correlate with stage and outcome in various tumors. The aim of the present study was to assess expression patterns and the prognostic role of TKTL1 in muscle-invasive bladder cancer (MIBC).

Patients and methods

The expression of TKTL1 was assessed in a tissue microarray consisting of histopathologically benign and malign tissue of 112 patients who underwent radical cystectomy due to MIBC. Cytoplasmatic and nuclear expression were assessed by immunohistochemistry and compared separately with clinicopathologic parameters and outcome.

Results

Cytoplasmatic expression of TKTL1 was exclusively present in tumor tissue. In contrast, the proportion of nuclei positive for TKTL1 was higher in histopathologically benign tissue compared with malign tissue. No correlation was observed between cytoplasmatic or nuclear TKTL1 expression and tumor stage, grade or the presence of metastases. Patients with lymph node involvement showed a decreased frequency of cytoplasmatic expression compared with node-negative patients (p = 0.01). However, no further correlation was observed between the expression of TKTL1 and clinical outcome of patients.

Conclusions

The present study shows that the cytoplasmatic expression of TKTL1 is specific for MIBC tissue compared with histopathologically benign urothelium. This specific expression is present in a subgroup of MIBC potentially identifying patients with activated PPP suitable for a targeted inhibition of sugar metabolism. In contrast to other malignancies, TKTL1 shows no prognostic significance in MIBC.

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Title: Expression patterns and prognostic role of transketolase-like 1 in muscle-invasive bladder cancer
Authors: Mauro Semilia
Jörg Hennenlotter
Carlo Pavone
Teresa Bischoff
Ursula Kühs
Georgios Gakis
Jens Bedke
Arnulf Stenzl
Christian Schwentner
Tilman Todenhöfer
Publication date: 01-10-2015
Publisher: Springer Berlin Heidelberg
Published in: World Journal of Urology / Issue 10/2015
Print ISSN: 0724-4983
Electronic ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-014-1473-4

At a glance: The STEP trials

Springer Medicine

Expression patterns and prognostic role of transketolase-like 1 in muscle-invasive bladder cancer

Abstract

Purpose

Patients and methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Patients and methods

Results

Conclusions

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