Top

European Archives of Oto-Rhino-Laryngology

Published in:

01-03-2013 | Rhinology

Expression of the cysteinyl leukotriene 1 receptor and glucocorticoid receptor-β in nasal polyps

Authors: Yong-Hwi An, Sung-Lyong Hong, Doo Hee Han, Chul Hee Lee, Yang-Gi Min, Chae-Seo Rhee

Published in: European Archives of Oto-Rhino-Laryngology | Issue 4/2013

Abstract

The objective of this study was to analyze the expression of cysteinyl leukotriene 1 (CysLT1) receptor and glucocorticoid receptors (GRs) in nasal polyps, and to evaluate the relationship between the expression of CysLT1 receptors and that of GRs. Nasal polyps were taken from 32 patients of chronic rhinosinusitis with nasal polyposis. Samples of middle turbinate from seven healthy subjects were used as controls. Specimens were immunohistochemically stained for CysLT1 receptor, GR-α and GR-β receptor, and were quantified in the unit area of the tissues. Numbers of CysLT1 receptor-positive cells were much increased in nasal polyps than in middle turbinate (281 ± 67 vs. 157 ± 85 cells/mm², P = .01). There was no significant difference in the numbers of GR-α positive cells between nasal polyps and normal turbinate mucosa. GR-β positive cells were increased in nasal polyps as compared to normal turbinate mucosa (36 ± 8 vs. 19 ± 7 cells/mm², P = .03). A significant relationship was found between the expression of CysLT1 receptor and GR-β in nasal polyps (R = .525, P = .04), whereas there was no significant relationship between the expression of CysLT1 receptor and GR-α in nasal polyps. Our study shows that CysLT1 receptor expression predominates on GR-β over-expressed polyps. This may suggest the additional effect of CysLT1 receptor antagonist for the treatment of nasal polyposis resistant to steroid alone.

Mygind N (1990) Nasal polyposis. J Allergy Clin Immunol 86:827–829PubMedCrossRef

Hamilos DL, Leung DY, Muro S et al (2001) GRbeta expression in nasal polyp inflammatory cells and its relationship to the anti-inflammatory effects of intranasal fluticasone. J Allergy Clin Immunol 108:59–68PubMedCrossRef

Wang M, Shi P, Chen B, Shi G, Li H, Wang H (2011) Superantigen-induced glucocorticoid insensitivity in the recurrence of chronic rhinosinusitis with nasal polyps. Otolaryngol Head Neck Surg 145:717–722PubMedCrossRef

Christodoulopoulos P, Cameron L, Durham S, Hamid Q (2000) Molecular pathology of allergic disease II: upper airway disease. J Allergy Clin Immunol 105:211–223PubMedCrossRef

Currie GP, Lee DK, Srivastava P (2005) Long-acting bronchodilator or leukotriene modifier as add-on therapy to inhaled corticosteroids in persistent asthma? Chest 128:2954–2962PubMedCrossRef

Ducharme FM (2011) Leukotriene receptor antagonists as first line or add-on treatment for asthma. BMJ 13:343

Nagata M, Saito K (2003) The roles of cysteinyl leukotrienes in eosinophilic inflammation of asthmatic airways. Int Arch Allergy Immunol 131:7–10PubMedCrossRef

Chao SS, Graham SM, Brown CL, Kline JN, Hussain I (2006) Cysteinyl leukotriene 1 receptor expression in nasal polyps. Ann Otol Rhinol Laryngol 115:394–397PubMed

Rasp G (2010) Is there a role for leukotriene antagonists in the prevention of recurrent nasal polyps? Curr Opin Allergy Clin Immunol 10:200–205PubMedCrossRef

10.

Hui Y, Funk CD (2002) Cysteinyl leukotriene receptors. Biochem Pharmacol 64:1549–1557PubMedCrossRef

11.

Lund VJ, Mackay IS (1993) Staging in rhinosinusitis. Rhinology 31:183–184PubMed

12.

Goleva E, Li LB, Eves PT, Strand MJ, Martin RJ, Leung DY (2006) Increased glucocorticoid receptor beta alters steroid response in glucocorticoid-insensitive asthma. Am J Respir Crit Care Med 173:607–616PubMedCrossRef

13.

Sousa AR, Parikh A, Scadding G, Corrigan CJ, Lee TH (2002) Leukotriene-receptor expression on nasal mucosal inflammatory cells in aspirin-sensitive rhinosinusitis. N Engl J Med 347:1524–1526CrossRef

14.

Shirasaki H, Kanaizumi E, Watanabe K et al (2002) Expression and localization of the cysteinyl leukotriene 1 receptor in human nasal mucosa. Clin Exp Allergy 32:1007–1012PubMedCrossRef

15.

Adamusiak AM, Stasikowska-Kanicka O, Lewandowska-Polak A et al (2011) Expression of arachidonate metabolism enzymes and receptors in nasal polyps of aspirin-hypersensitive asthmatics. Int Arch Allergy Immunol 157:354–362PubMedCrossRef

16.

Oakley RH, Jewell CM, Yudt MR, Bofetiado DM, Cidlowski JA (1999) The dominant negative activity of the human glucocorticoid receptor beta isoform. Specificity and mechanisms of action. J Biol Chem 274:27857–27866PubMedCrossRef

17.

Pujols L, Mullol J, Benítez P et al (2003) Expression of the glucocorticoid receptor alpha and beta isoforms in human nasal mucosa and polyp epithelial cells. Respir Med 97:90–96PubMedCrossRef

18.

Fakhri S, Tulic M, Christodoulopoulos P et al (2004) Microbial superantigens induce glucocorticoid receptor beta and steroid resistance in a nasal explant model. Laryngoscope 114:887–892PubMedCrossRef

19.

de Wang Y (2008) Significance of susceptible gene expression profiles in nasal polyposis. Clin Exp Otorhinolaryngol 1:177–183CrossRef

20.

Hamid QA, Wenzel SE, Hauk PJ et al (1999) Increased glucocorticoid receptor beta in airway cells of glucocorticoid-insensitive asthma. Am J Respir Crit Care Med 159:1600–1604PubMed

21.

Sousa AR, Lane SJ, Cidlowski JA, Staynov DZ, Lee TH (2000) Glucocorticoid resistance in asthma is associated with elevated in vivo expression of the glucocorticoid receptor b-isoform. J Allergy Clin Immunol 105:943–950PubMedCrossRef

22.

Honda M, Orii F, Ayabe T et al (2000) Expression of glucocorticoid receptor beta in lymphocytes of patients with glucocorticoid-resistant ulcerative colitis. Gastroenterology 118:859–866PubMedCrossRef

23.

Shahidi H, Vottero A, Stratakis CA et al (1999) Imbalanced expression of the glucocorticoid receptor isoforms in cultured lymphocytes from a patient with systemic glucocorticoid resistance and chronic lymphocytic leukemia. Biochem Biophys Res Commun 254:559–565PubMedCrossRef

24.

Gagliardo R, Chanez P, Vignola AM et al (2000) Glucocorticoid receptor alpha and beta in glucocorticoid dependent asthma. Am J Respir Crit Care Med 162:7–13PubMed

25.

Pujols L, Alobid I, Benítez P et al (2008) Regulation of glucocorticoid receptor in nasal polyps by systemic and intranasal glucocorticoids. Allergy 63:1377–1386PubMedCrossRef

26.

Watanabe S, Suzaki H (2008) Changes of glucocorticoid receptor expression in the nasal polyps of patients with chronic sinusitis following treatment with glucocorticoid. In Vivo 22:37–42PubMed

27.

Yu M, Watanabe S, Kudo M, Kanai K, Suzaki H (2009) Glucocorticoid receptor immunoreactivity of eosinophils in nasal polyps. Acta Otolaryngol Suppl 562:95–100PubMedCrossRef

28.

Parnes SM, Chuma AV (2000) Acute effects of antileukotrienes on sinonasal polyposis and sinusitis. Ear Nose Throat J 79(18–20):24–25

29.

Stewart RA, Ram B, Hamilton G, Weiner J, Kane KJ (2008) Montelukast as an adjunct to oral and inhaled steroid therapy in chronic nasal polyposis. Otolaryngol Head Neck Surg 139:682–687PubMedCrossRef

Title: Expression of the cysteinyl leukotriene 1 receptor and glucocorticoid receptor-β in nasal polyps
Authors: Yong-Hwi An
Sung-Lyong Hong
Doo Hee Han
Chul Hee Lee
Yang-Gi Min
Chae-Seo Rhee
Publication date: 01-03-2013
Publisher: Springer-Verlag
Published in: European Archives of Oto-Rhino-Laryngology / Issue 4/2013
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-012-2239-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Expression of the cysteinyl leukotriene 1 receptor and glucocorticoid receptor-β in nasal polyps

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 4/2013

The effect of intratympanic vitamin C administration on cisplatin-induced ototoxicity

Predictors of surgical approaches for the repair of anterior cranial base encephaloceles

Functional evaluation of paratubal muscles using electromyography in patients with chronic unilateral tubal dysfunction

Eric M. Genden: reconstruction of the head and neck. A defect-oriented approach, ISBN: 978-1-60406-576-3

Evaluating d-methionine dose to attenuate oxidative stress-mediated hearing loss following overexposure to noise

The temporo-parietal fascial flap in extended transnasal endoscopic procedures: cadaver dissection and personal clinical experience