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International Journal of Colorectal Disease

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01-01-2010 | Original Article

Expression of putative stem cell genes Musashi-1 and β1-integrin in human colorectal adenomas and adenocarcinomas

Authors: Li-Fang Fan, Wei-Guo Dong, Cong-Qing Jiang, Dong Xia, Fei Liao, Qiong-Fang Yu

Published in: International Journal of Colorectal Disease | Issue 1/2010

Abstract

Background and aims

Recent studies revealed that Musashi-1and β1-integrin were putative stem cell genes. Overexpressions of Musashi-1 and β1-integrin have been reported in some tumor tissues and cell lines. This study was to detect expressions of the two genes in colorectal adenomas and carcinomas and to analyze the correlation between Musashi-1 and β1-integrin.

Methods

Musashi-1 and β1-integrin immunoreactivity was studied immunohistochemically in tissue microarray-based samples containing 69 colorectal adenocarcinomas, eight normal mucosa, and eight adenomas, and their messenger RNA (mRNA) expression level was detected by RT-PCR in resected specimens including the three types of tissue.

Results

A percentage of 66.7% (46/69) and 59.2% (41/69) of colorectal adenocarcinomas were immunoreactive with Musashi-1 and β1-integrin, respectively. The expressions of Musashi-1 and β1-integrin protein were significantly higher in tissue samples of stage III than those of stage I-II (P = 0.0252; P = 0.0018, respectively). β1-integrin expression was higher in group of adenocarcinomas than that of adenomas (P = 0.0276). Musashi-1 expression was closely correlated with β1-integrin (rs = 0.631, P = 0.0001). Significant differences of Musashi-1 and β1-integrin mRNA expression levels were found between the normal colorectal mucosa, adenoma, and adenocarcinoma tissues (P = 0.01; P = 0.03, respectively).

Conclusions

Musashi-1 and β1-integrin may be involved in human colorectal tumor carcinogenesis and progression. Our observations also indicate the need for further investigations to test in vivo whether cells with these markers have stem cell properties.

Mimeault M, Batra SK (2007) Functions of tumorigenic and migrating cancer progenitor cells in cancer progression and metastasis and their therapeutic implications. Cancer Metastasis Rev 26:203–214CrossRefPubMed

Pathak S (2002) Organ- and tissue-specific stem cells and carcinogenesis. Anticancer Res 22:1353–1356PubMed

Li L, Neaves WB (2006) Normal stem cells and cancer stem cells: the niche matters. Cancer Res 66:4553–4557CrossRefPubMed

Kurihara Y, Nagata T, Imai T et al (1997) Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1. Gene 186:21–27CrossRefPubMed

Okano H, Kawahara H, Toriya M et al (2005) Function of RNA-binding protein Musashi-1 in stem cells. Exp Cell Res 306:349–356CrossRefPubMed

Potten CS, Booth C, Tudor GL et al (2003) Identification of a putative intestinal stem cell and early lineage marker: musashi-1. Differentiation 7:128–141

Sureban SM, May R, George RJ et al (2008) Knockdown of RNA binding protein musashi-1 leads to tumor regression in vivo. Gastroenterology 134:1448–1458CrossRefPubMed

Yokota N, Mainprize TG, Taylor MD et al (2004) Identification of differentially expressed and developmentally regulated genes in medulloblastoma using suppression subtraction hybridization. Oncogenes 23:3444–3453CrossRef

Nakano A, Kanemura Y, Mori K et al (2007) Expression of the neural RNA-binding protein Musashi1 in pediatric brain tumors. Pediatr Neurosurg 43:279–284CrossRefPubMed

10.

Toda M, Iizuka Y, Yu W et al (2001) Stem cells, cancer, and cancer stem cells. Expression of the neural RNA-binding protein Musashi1 in human gliomas. Glia 34:1–7CrossRefPubMed

11.

Kanemura Y, Mori K, Sakakibara S et al (2001) Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity. Differentiation 68:141–152CrossRefPubMed

12.

Ma YH, Mentlein R, Knerlich F, Kruse ML et al (2008) Expression of stem cell markers in human astrocytomas of different WHO grades. J Neurooncol 86:31–45CrossRefPubMed

13.

Seigel GM, Hackam AS, Ganguly A et al (2007) Human embryonic and neuronal stem cell markers in retinoblastoma. Mol Vis 13:823–832PubMed

14.

Götte M, Wolf M, Staebler A, Buchweitz O, Kelsch R, Schüring A, Kiesel L (2008) Increased expression of the adult stem cell marker Musashi-1 in endometriosis and endometrial carcinoma. J Pathol 215:317–329CrossRefPubMed

15.

Shu HJ, Saito T, Watanabe H, Ito JI et al (2002) Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines. Biochem Biophys Res Commun 293:150–154CrossRefPubMed

16.

Schulenburg A, Cech P, Herbacek I et al (2007) Ulrich-Pur, CD44-positive colorectal adenoma cells express the potential stem cell markers musashi antigen (msi1) and ephrin B2 receptor (EphB2). J Pathol 213:152–160CrossRefPubMed

17.

Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110:673–687CrossRefPubMed

18.

Fujimoto K, Beauchamp RD, Whitehead RH (2002) Identification and isolation of candidate human colonic clonogenic cells based on cell surface integrin expression. Gastroenterology 123:1941–1948CrossRefPubMed

19.

Reya T, Morrison SJ, Clarke MF et al (2001) Stem cells, cancer, and cancer stem cells. Nature 414:105–111CrossRefPubMed

20.

Fan LF, Dong WG, Jiang CQ et al (2008) Role of Hypoxia-inducible factor-1alpha and Survivin in colorectal carcinoma progression. Int J Colorectal Dis 23:1057–1064CrossRefPubMed

21.

Bouchet BY, Colón M, Polotsky A et al (2000) Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture. J Biomed Mater Res 52:716–724CrossRefPubMed

22.

Nishimura S, Wakabayashi N, Toyoda K et al (2003) Expression of Musashi-1 in human normal colon crypt cells: a possible stem cell marker of human colon epithelium. Dig Dis Sci 48:1523–1529CrossRefPubMed

23.

Giancotti FG, Ruoslahti E (1999) Integrin signaling. Science 285:1028–1032CrossRefPubMed

24.

Jones RG, Li X, Gray PD et al (2006) Conditional deletion of beta1 integrins in the intestinal epithelium causes a loss of Hedgehog expression, intestinal hyperplasia, and early postnatal lethality. J Cell Biol 175:505–514CrossRefPubMed

25.

Dimitriadis E, Trangas T, Milatos S et al (2007) Expression of oncofetal RNA-binding protein CRD-BP/IMP1 predicts clinical outcome in colon cancer. Int J Cancer 121:486–494CrossRefPubMed

Title: Expression of putative stem cell genes Musashi-1 and β1-integrin in human colorectal adenomas and adenocarcinomas
Authors: Li-Fang Fan
Wei-Guo Dong
Cong-Qing Jiang
Dong Xia
Fei Liao
Qiong-Fang Yu
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 1/2010
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-009-0791-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Expression of putative stem cell genes Musashi-1 and β1-integrin in human colorectal adenomas and adenocarcinomas

Abstract

Background and aims

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background and aims

Methods

Results

Conclusions

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