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Journal of Assisted Reproduction and Genetics

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01-02-2013 | Gamete Biology

Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation

Authors: Yi-Ran Li, Chun-E Ren, Quan Zhang, Ji-Chun Li, Ri-Cheng Chian

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2013

Abstract

Purpose

To determine expression of G-protein estrogen receptor (GPER) in mouse oocyte membrane during maturation.

Methods

The expression of GPER from different maturation stages of oocytes, in vivo and in vitro matured oocytes as well as aging oocytes was examined by immune-fluorescence GPR30 antibody and the images were analyzed by laser scanning confocal microscope. Further confirmation was performed by Western blots for cell fractionation.

Results

Significant fluorescent signal was observed on the surface of mouse oocytes. The image expression was lower in germinal vesicle (GV) stage than mature metaphase-II (M-II) stage oocytes. There was high expression in in-vivo matured oocytes compared to in vitro matured oocytes. The highest expression was observed in aging oocytes compared with other oocytes.

Conclusions

The changes of expression of GPER on mouse oocytes plasma membrane confirm oocyte membrane maturation, suggesting that those changes of GPER may be related to the functional role of oocyte maturation.

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Title: Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation
Authors: Yi-Ran Li
Chun-E Ren
Quan Zhang
Ji-Chun Li
Ri-Cheng Chian
Publication date: 01-02-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-9942-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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