Top

Published in:

01-11-2014 | Original Article

Expression of DNA repair genes in burned skin exposed to low-level red laser

Authors: Eduardo Tavares Lima Trajano, Andre Luiz Mencalha, Andréa Monte-Alto-Costa, Luís Cristóvão Pôrto, Adenilson de Souza da Fonseca

Published in: Lasers in Medical Science | Issue 6/2014

Abstract

Although red laser lights lie in the region of non-ionizing radiations in the electromagnetic spectrum, there are doubts whether absorption of these radiations causes lesions in the DNA molecule. Our aim was to investigate the expression of the genes involved with base excision and nucleotide excision repair pathways in skin tissue submitted to burn injury and exposed to low-level red laser. Wistar rats were divided as follows: control group—rats burned and not irradiated, laser group—rats burned and irradiated 1 day after injury for five consecutive days, and later laser group—rats injured and treated 4 days after injury for five consecutive days. Irradiation was performed according to a clinical protocol (20 J/cm², 100 mW, continuous wave emission mode). The animals were sacrificed on day 10, and scarred tissue samples were withdrawn for total RNA extraction, complementary DNA (cDNA) synthesis, and evaluation of gene expression by quantitative polymerase chain reaction. Low-level red laser exposure (1) reduces the expression of APE1 messenger (mRNA), (2) increases the expression of OGG1 mRNA, (3) reduces the expression of XPC mRNA, and (4) increases the expression of XPA mRNA both in laser and later laser groups. Red laser exposure at therapeutic fluences alters the expression of genes related to base excision and nucleotide excision pathways of DNA repair during wound healing of burned skin.

Peck MD (2011) Epidemiology of burns throughout the world. Part I: distribution and risk factors. Burns 37:1087–1100PubMedCrossRef

Karami Matin B, Karami Matin R, Ahmadi Joybari T, Ghahvehei N, Haghi M, Ahmadi M, Rezaei S (2012) Epidemiological data, outcome, and costs of burn patients in Kermanshah. Ann Burns Fire Disasters 25:171–177PubMedPubMedCentral

Castana O, Anagiotos G, Dagdelenis J, Tsagoulis N, Giannakidou M, Roidi D, Alexakis D (2008) Epidemiological survey of burn victims treated as emergency cases in our hospital in the last five years. Ann Burns Fire Disasters 21:71–74

Niemz MH (2007) Laser–tissue interactions: fundamentals and applications. Springer, New York

Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomed Laser Surg 28:291–325PubMedCrossRef

Nunez SC, Franca CM, Silva DF, Nogueira GE, Prates RA, Ribeiro MS (2013) The influence of red laser irradiation timeline on burn healing in rats. Lasers Med Sci 28:633–641PubMedCrossRef

Bayat M, Vasheghani MM, Razavie N, Jalili MR (2008) Effects of low-level laser therapy on mast cell number and degranulation in third-degree burns of rats. J Rehabil Res Dev 45:931–938PubMedCrossRef

da Silva JP, da Silva MA, Almeida AP, Lombardi Junior I, Matos AP (2010) Laser therapy in the tissue repair process: a literature review. Photomed Laser Surg 28:17–21PubMedCrossRef

Karu T, Pyatibrat L, Kalendo G (1994) Irradiation with He–Ne laser can influence the cytotoxic response of HeLa cells to ionizing radiation. Int J Radiat Biol 65:691–697PubMedCrossRef

10.

Kohli R, Gupta PK, Dube A (2000) Helium-neon laser preirradiation induces protection against UVC radiation in wild-type E. coli strain K12AB1157. Radiat Res 153:181–185PubMedCrossRef

11.

Fonseca AS, Moreira TO, Paixao DL, Farias FM, Guimaraes OR, de Paoli S, Geller M, de Paoli F (2010) Effect of laser therapy on DNA damage. Lasers Surg Med 42:481–488PubMedCrossRef

12.

da Silva Marciano R, da Silva Sergio LP, Polignano GA, Presta GA, Guimaraes OR, Geller M, de Paoli S, de Paoli F, da Fonseca Ade S (2012) Laser for treatment of aphthous ulcers on bacteria cultures and DNA. Photochem Photobiol Sci 11:1476–1483PubMedCrossRef

13.

Zhang Y, Song S, Fong CC, Tsang CH, Yang Z, Yang M (2003) cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light. J Invest Dermatol 120:849–857PubMedCrossRef

14.

de Souza da Fonseca A, Mencalha AL, Araujo de Campos VM, Ferreira Machado SC, de Freitas Peregrino AA, Geller M, de Paoli F (2013) DNA repair gene expression in biological tissues exposed to low-intensity infrared laser. Lasers Med Sci 28:1077–1084PubMedCrossRef

15.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408PubMedCrossRef

16.

Parihar A, Parihar MS, Milner S, Bhat S (2008) Oxidative stress and anti-oxidative mobilization in burn injury. Burns 34:6–17PubMedCrossRef

17.

Klungland A, Bjelland S (2007) Oxidative damage to purines in DNA: role of mammalian Ogg1. DNA Repair (Amst) 6:481–488CrossRef

18.

Russo MT, De Luca G, Degan P, Bignami M (2007) Different DNA repair strategies to combat the threat from 8-oxoguanine. Mutat Res 614:1–2CrossRef

19.

Hazra TK, Das A, Das S, Choudhury S, Kow YW, Roy R (2007) Oxidative DNA damage repair in mammalian cells: a new perspective. DNA Repair (Amst) 6:470–480CrossRef

20.

Karu TI (2010) Multiple roles of cytochrome c oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life 62:607–610PubMedCrossRef

21.

Goosen N (2010) Scanning the DNA for damage by the nucleotide excision repair machinery. DNA Repair (Amst) 9:593–596CrossRef

22.

Berquist BR, Wilson DM (2012) Pathways for repairing and tolerating the spectrum of oxidative DNA lesions. Cancer Lett 327:61–72PubMedCrossRefPubMedCentral

23.

Sugasawa K (2011) Multiple DNA damage recognition factors involved in mammalian nucleotide excision repair. Biochemistry (Mosc) 76:16–23CrossRef

24.

Brosh RM Jr (2013) DNA helicases involved in DNA repair and their roles in cancer. Nat Rev Cancer 13:542–558PubMedCrossRef

25.

Melis JP, van Steeg H, Luijten M (2013) Oxidative DNA damage and nucleotide excision repair. Antioxid Redox Signal 18:2409–2419PubMedCrossRefPubMedCentral

26.

Singh V, Devgan L, Bhat S, Milner SM (2007) The pathogenesis of burn wound conversion. Ann Plast Surg 59:109–15PubMedCrossRef

Title: Expression of DNA repair genes in burned skin exposed to low-level red laser
Authors: Eduardo Tavares Lima Trajano
Andre Luiz Mencalha
Andréa Monte-Alto-Costa
Luís Cristóvão Pôrto
Adenilson de Souza da Fonseca
Publication date: 01-11-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 6/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1612-6

At a glance: The STEP trials

Springer Medicine

Expression of DNA repair genes in burned skin exposed to low-level red laser

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2014

Adjunctive use of combination of super-pulsed laser and light-emitting diodes phototherapy on nonspecific knee pain: double-blinded randomized placebo-controlled trial

Effect of low-level laser therapy on repair of the bone compromised by radiotherapy

Effect of acetic acid on optical coherence tomography (OCT) images of cervical epithelium

Acute effects of low-level laser therapy on physiologic and electromyographic responses to the cardiopulmonary exercise testing in healthy untrained adults

Optical feedback-induced light modulation for fiber-based laser ablation

Raman spectroscopic study of the repair of surgical bone defects grafted or not with biphasic synthetic micro-granular HA + β-calcium triphosphate irradiated or not with λ850 nm LED light