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Pediatric Nephrology
Published in: Pediatric Nephrology 3/2011

01-03-2011 | Original Article

Expression and function of the Ets transcription factor pea3 during formation of zebrafish pronephros

Authors: Qiuxia Chen, Songming Huang, Qingshun Zhao, Ronghua Chen, Aihua Zhang

Published in: Pediatric Nephrology | Issue 3/2011

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Abstract

Polyomavirus enhancer activator 3 (Pea3), belonging to the PEA3 subfamily of Ets transcription factors, is essential for certain organogenesis in mammals. Previously, we found that pea3 correlated with wt1 expression and may contribute to nephrogenesis in rats. Here, we observed that pea3 was mainly expressed in the zebrafish pronephric glomerulus. We further performed functional analyses by in situ hybridization of pea3 in zebrafish embryos after pea3 messenger RNA (mRNA) overexpression and inhibition of double-target genes (pea3 and erm, another member of the PEA3 subfamily) by antisense morpholino-oligonucleotides (MO). Overexpression of pea3 induced abnormal pronephrogenesis. However, MO-pea3 coinjected with MO-erm, but not alone, inhibited zebrafish pronephros development, and these defects were rescued by overexpression of the zebrafish wt1a gene. Thus, pea3 and erm are required for zebrafish pronephrogenesis and can functionally complement each other, and the wt1a gene may be one of their downstream targets.
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Metadata
Title
Expression and function of the Ets transcription factor pea3 during formation of zebrafish pronephros
Authors
Qiuxia Chen
Songming Huang
Qingshun Zhao
Ronghua Chen
Aihua Zhang
Publication date
01-03-2011
Publisher
Springer-Verlag
Published in
Pediatric Nephrology / Issue 3/2011
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI
https://doi.org/10.1007/s00467-010-1713-9

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