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01-06-2018 | Original Paper

Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues

Authors: Nanako Hamada, Makoto Mizuno, Hiroyuki Tomita, Ikuko Iwamoto, Akira Hara, Koh-ichi Nagata

Published in: Medical Molecular Morphology | Issue 2/2018

Abstract

Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton. From the obtained results, anti-Dusp22 was found to be a useful tool for biochemical and cell biological analyses of Dusp22.

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Title: Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues
Authors: Nanako Hamada
Makoto Mizuno
Hiroyuki Tomita
Ikuko Iwamoto
Akira Hara
Koh-ichi Nagata
Publication date: 01-06-2018
Publisher: Springer Japan
Published in: Medical Molecular Morphology / Issue 2/2018
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI: https://doi.org/10.1007/s00795-017-0178-3

At a glance: The STEP trials

Springer Medicine

Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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