Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Gut Pathogens
Published in: Gut Pathogens 1/2013

Open Access 01-12-2013 | Genome Announcement

Evidence of a new metabolic capacity in an emerging diarrheal pathogen: lessons from the draft genomes of Vibrio fluvialis strains PG41 and I21563

Authors: Indu Khatri, Sakshi Mahajan, Chetna Dureja, Srikrishna Subramanian, Saumya Raychaudhuri

Published in: Gut Pathogens | Issue 1/2013

Login to get access

Abstract

Background

Vibrio fluvialis is an emerging diarrheal pathogen for which no genome is currently available. In this work, draft genomes of two closely related clinical strains PG41 and I21563 have been explored.

Results

V. fluvialis strains PG41 and I21563 were sequenced on the Illumina HiSeq 1000 platform to obtain draft genomes of 5.3 Mbp and 4.4 Mbp respectively. Our genome data reveal the presence of genes involved in ethanolamine utilization, which is further experimentally confirmed by growth analysis.

Conclusions

Combined in silico and growth analysis establish a new metabolic capacity of V. fluvialis to harvest energy from ethanolamine.
Appendix
Available only for authorised users
Literature
1.
Haley BJ, Grim CJ, Hasan NA, Choi SY, Chun J, Brettin TS, Bruce DC, Challacombe JF, Detter JC, Han CS: Comparative genomic analysis reveals evidence of two novel Vibrio species closely related to V cholerae. BMC Microbiol. 2010, 10: 154-10.1186/1471-2180-10-154.PubMedCentralCrossRefPubMed
2.
Morris JG: Cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell. Clin Infect Dis. 2003, 37: 272-280. 10.1086/375600.CrossRefPubMed
3.
4.
5.
Nair GB, Ramamurthy T, Bhattacharya SK, Dutta B, Takeda Y, Sack DA: Global dissemination of Vibrio parahaemolyticus serotype O3:K6 and its serovariants. Clin Microbiol Rev. 2007, 20: 39-48.PubMedCentralCrossRefPubMed
6.
Chitov T, Kirikaew P, Yungyune P, Ruengprapan N, Sontikun K: An incidence of large foodborne outbreak associated with Vibrio mimicus. Eur J Clin Microbiol Infect Dis. 2009, 28: 421-424. 10.1007/s10096-008-0639-7.CrossRefPubMed
7.
Chowdhury G, Pazhani GP, Dutta D, Guin S, Dutta S, Ghosh S, Izumiya H, Asakura M, Yamasaki S, Takeda Y: Vibrio fluvialis in patients with diarrhea, Kolkata, India. Emerg Infect Dis. 2012, 18: 1868-1871. 10.3201/eid1811.120520.PubMedCentralCrossRefPubMed
8.
Lee JV, Shread P, Furniss AL, Bryant TN: Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F vibrios, group EF6). J Appl Bacteriol. 1981, 50: 73-94. 10.1111/j.1365-2672.1981.tb00873.x.CrossRefPubMed
9.
Igbinosa EO, Okoh AI: Vibrio fluvialis: an unusual enteric pathogen of increasing public health concern. Int J Environ Res Public Health. 2010, 7: 3628-3643. 10.3390/ijerph7103628.PubMedCentralCrossRefPubMed
10.
Huq MI, Alam AK, Brenner DJ, Morris GK: Isolation of Vibrio-like group, EF-6, from patients with diarrhea. J Clin Microbiol. 1980, 11: 621-624.PubMedCentralPubMed
11.
Bellet J, Klein B, Altieri M, Ochsenschlager D: Vibrio fluvialis, an unusual pediatric enteric pathogen. Pediatr Emerg Care. 1989, 5: 27-28. 10.1097/00006565-198903000-00008.CrossRefPubMed
12.
Lesmana M, Subekti DS, Tjaniadi P, Simanjuntak CH, Punjabi NH, Campbell JR, Oyofo BA: Spectrum of vibrio species associated with acute diarrhea in North Jakarta, Indonesia. Diagn Microbiol Infect Dis. 2002, 43: 91-97. 10.1016/S0732-8893(02)00373-5.CrossRefPubMed
13.
Bhattacharjee S, Bhattacharjee S, Bal B, Pal R, Niyogi SK, Sarkar K: Is Vibrio fluvialis emerging as a pathogen with epidemic potential in coastal region of eastern India following cyclone Aila?. J Health Popul Nutr. 2010, 28: 311-317.PubMedCentralPubMed
14.
Allton DR, Forgione MA, Gros SP: Cholera-like presentation in Vibrio fluvialis enteritis. South Med J. 2006, 99: 765-767. 10.1097/01.smj.0000223657.22296.e6.CrossRefPubMed
15.
Kothary MH, Lowman H, McCardell BA, Tall BD: Purification and characterization of enterotoxigenic El Tor-like hemolysin produced by Vibrio fluvialis. Infect Immun. 2003, 71: 3213-3220. 10.1128/IAI.71.6.3213-3220.2003.PubMedCentralCrossRefPubMed
16.
Chakraborty R, Chakraborty S, De K, Sinha S, Mukhopadhyay AK, Khanam J, Ramamurthy T, Takeda Y, Bhattacharya SK, Nair GB: Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea. J Med Microbiol. 2005, 54: 707-716. 10.1099/jmm.0.45820-0.CrossRefPubMed
17.
Ahmed AM, Shinoda S, Shimamoto T: A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis. FEMS Microbiol Lett. 2005, 242: 241-247. 10.1016/j.femsle.2004.11.012.CrossRefPubMed
18.
Chowdhury G, Pazhani GP, Nair GB, Ghosh A, Ramamurthy T: Transferable plasmid-mediated quinolone resistance in association with extended-spectrum beta-lactamases and fluoroquinolone-acetylating aminoglycoside-6’-N-acetyltransferase in clinical isolates of Vibrio fluvialis. Int J Antimicrob Agents. 2011, 38: 169-173.PubMed
19.
20.
Kofoid E, Rappleye C, Stojiljkovic I, Roth J: The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins. J Bacteriol. 1999, 181: 5317-5329.PubMedCentralPubMed
21.
22.
23.
Sawabe T, Kita-Tsukamoto K, Thompson FL: Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol. 2007, 189: 7932-7936. 10.1128/JB.00693-07.PubMedCentralCrossRefPubMed
24.
Boetzer M, Henkel CV, Jansen HJ, Butler D, Pirovano W: Scaffolding pre-assembled contigs using SSPACE. Bioinformatics. 2011, 27: 578-579. 10.1093/bioinformatics/btq683.CrossRefPubMed
25.
Nadalin F, Vezzi F, Policriti A: GapFiller: a de novo assembly approach to fill the gap within paired reads. BMC Bioinforma. 2012, 13 Suppl 14: S8-CrossRef
26.
Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M: The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008, 9: 75-10.1186/1471-2164-9-75.PubMedCentralCrossRefPubMed
27.
28.
Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW: RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 2007, 35: 3100-3108. 10.1093/nar/gkm160.PubMedCentralCrossRefPubMed
29.
Pei J, Sadreyev R, Grishin NV: PCMA: fast and accurate multiple sequence alignment based on profile consistency. Bioinformatics. 2003, 19: 427-428. 10.1093/bioinformatics/btg008.CrossRefPubMed
30.
Milne I, Lindner D, Bayer M, Husmeier D, McGuire G, Marshall DF, Wright F: TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics. 2009, 25: 126-127. 10.1093/bioinformatics/btn575.PubMedCentralCrossRefPubMed
31.
Bertin Y, Girardeau JP, Chaucheyras-Durand F, Lyan B, Pujos-Guillot E, Harel J, Martin C: Enterohaemorrhagic Escherichia coli gains a competitive advantage by using ethanolamine as a nitrogen source in the bovine intestinal content. Environ Microbiol. 2011, 13: 365-377. 10.1111/j.1462-2920.2010.02334.x.CrossRefPubMed
32.
Kendall MM, Gruber CC, Parker CT, Sperandio V: Ethanolamine controls expression of genes encoding components involved in interkingdom signaling and virulence in enterohemorrhagic Escherichia coli O157:H7. MBio. 2012, 3
Metadata
Title
Evidence of a new metabolic capacity in an emerging diarrheal pathogen: lessons from the draft genomes of Vibrio fluvialis strains PG41 and I21563
Authors
Indu Khatri
Sakshi Mahajan
Chetna Dureja
Srikrishna Subramanian
Saumya Raychaudhuri
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Gut Pathogens / Issue 1/2013
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/1757-4749-5-20

Other articles of this Issue 1/2013

Gut Pathogens 1/2013 Go to the issue

Review

Commensal Clostridia: leading players in the maintenance of gut homeostasis

Research

Genome anatomy of the gastrointestinal pathogen, Vibrio parahaemolyticus of crustacean origin

Research

Metagenomic profile of gut microbiota in children during cholera and recovery

Short report

Diversified pattern of the human colorectal cancer microbiome

Research

Gleaning evolutionary insights from the genome sequence of a probiotic yeast Saccharomyces boulardii

Research

Uropathogenic specific protein gene, highly distributed in extraintestinal uropathogenic Escherichia coli, encodes a new member of H-N-H nuclease superfamily
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Image Credits