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01-09-2017 | Original Article

Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis

Authors: Mohammadjavad Fredoni, Mahdi Ghatrehsamani, Mohammad-amin Abdollahifar, Sahar Bayat, Mohammad Bayat

Published in: Lasers in Medical Science | Issue 7/2017

Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) on cancellous bone in rat models of ovariectomized induced osteoporosis (OVX-D) and glucocorticoid-induced osteoporosis (GIOP). The experiment comprised of nine groups. A group of healthy rats was used for baseline evaluations. The OVX-D rats were further divided into groups as follows: control rats with osteoporosis, OVX-D rats that received alendronate (1 mg/kg 60 days), OVX-D rats treated with pulsed wave laser (890 nm, 80 Hz, 900 s, 0.0061 W/cm², 5.5 J/cm², three times a week, 60 days), and OVX-D rats treated with alendronate + pulsed laser. Dexamethasone was administered to the remaining rats that were split into four groups: control, alendronate-treated rats, laser-treated rats, and GIOP rats treated with alendronate + laser. T12, L1, L2, and L3 vertebrae were subjected to laser. Results of the current study demonstrated that OVX-D and GIOP significantly decreased some stereological parameters, and type 1 collagen gene expression compared to the healthy group. There was a significant increase in osteoclast number in both OVX-D and glucocorticoid administration compared to the healthy group. However, the detrimental effect of the OVX-D procedure on bone was more serious than glucocorticoid administration. Results showed that laser alone had a detrimental effect on trabecular bone volume, and cortical bone volume in groups GIOP and OVX-D compared to those in the healthy group. Alendronate significantly improved total vertebral bone volume, trabecular bone volume, and cortical bone volume, in GIOP and OVX-D groups compared to the laser-treated groups. Furthermore, the alendronate + laser in OVX-D rats and GIOP rats produced significantly increased osteoblast number and type 1 collagen gene expression and caused a significant decrease in osteoclast number compared to the controls.

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Title: Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis
Authors: Mohammadjavad Fredoni
Mahdi Ghatrehsamani
Mohammad-amin Abdollahifar
Sahar Bayat
Mohammad Bayat
Publication date: 01-09-2017
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 7/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2278-7

At a glance: The STEP trials

Springer Medicine

Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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