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01-08-2013 | Research Article

Evaluation of Inflammatory Response to Acute Ischemia Using Near-Infrared Fluorescent Reactive Oxygen Sensors

Authors: Selena Magalotti, Tiffany P. Gustafson, Qian Cao, Dana R. Abendschein, Richard A. Pierce, Mikhail Y. Berezin, Walter J. Akers

Published in: Molecular Imaging and Biology | Issue 4/2013

Abstract

Purpose

Ischemia-related processes associated with the generation of inflammatory molecules such as reactive oxygen species (ROS) are difficult to detect at the acute stage before the physiologic and anatomic evidence of tissue damage is present. Evaluation of the inflammatory and healing response early after an ischemic event in vivo will aid in treatment selection and patient outcomes. We introduce a novel near-infrared hydrocyanine molecular probe for the detection of ROS as a marker of tissue response to ischemia and a precursor to angiogenesis and remodeling. The synthesized molecular probe, initially a non-fluorescent hydrocyanine conjugated to polyethylene glycol, converts to a highly fluorescent cyanine reporter upon oxidation.

Procedures

The probe was applied in a preclinical mouse model for myocardial infarction, where ligation and removal of a portion of the femoral artery in the hindlimb resulted in temporary ischemia followed by angiogenesis and healing.

Results

The observed increase in fluorescence intensity was approximately sixfold over 24 h in the ischemic tissue relative to the uninjured control limb and was attributed to the higher concentration of ROS in the ischemic tissue.

Conclusions

These results demonstrate the potential for non-invasive sensing for interrogating the inflammatory and healing response in ischemic tissue.

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Title: Evaluation of Inflammatory Response to Acute Ischemia Using Near-Infrared Fluorescent Reactive Oxygen Sensors
Authors: Selena Magalotti
Tiffany P. Gustafson
Qian Cao
Dana R. Abendschein
Richard A. Pierce
Mikhail Y. Berezin
Walter J. Akers
Publication date: 01-08-2013
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 4/2013
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-013-0614-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluation of Inflammatory Response to Acute Ischemia Using Near-Infrared Fluorescent Reactive Oxygen Sensors

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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